LOCUS       sequence-107349-         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of HSPB6  (small HSP) tagged with V5 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Vos MJ, Zijlstra MP, Kanon B, van Waarde-Verhagen MA, Brunt ER, 
            Oosterveld-Hut HM, Carra S, Sibon OC, Kampinga HH
  TITLE     HSPB7 is the most potent polyQ aggregation suppressor within the 
            HSPB family of molecular chaperones.
  JOURNAL   Hum Mol Genet. 2010 Dec 1;19(23):4677-93. doi: 10.1093/hmg/ddq398. 
            Epub 2010 Sep 15.
  PUBMED    20843828
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/hmg/ddq398"; journalName: "Hum Mol Genet"; date: 
            "2010-12-1- 1"; volume: "19"; issue: "23"; pages: "4677-93"
COMMENT     Sequence Label: Frt-V5-hspB6
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    20..38
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     48..72
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             175..216
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(813..830)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 cctatcagtg atagagatct ccctatcagt gatagagatc gtcgacgagc tcgtttagtg
       61 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
      121 gaccgatcca gcctccggac tctagcgttt aaacttaagc ttgaattcac catgggtaag
      181 cctatcccta accctctcct cggtctcgat tctacgggat ccaaaacctc gagaaccatg
      241 gagatccctg tgcctgtgca gccgtcttgg ctgcgccgcg cctcggcccc gttgcccgga
      301 ctttcggcgc ccggacgcct ctttgaccag cgcttcggcg aggggctgct ggaggccgag
      361 ctggctgcgc tctgccccac cacgctcgcc ccctactacc tgcgcgcacc cagcgtggcg
      421 ctgcccgtcg cccaggtgcc gacggacccc ggccactttt cggtgctgct agacgtgaag
      481 cacttctcgc cggaggaaat tgctgtcaag gtggtgggcg aacacgtgga ggtgcacgcg
      541 cgccacgagg agcgcccgga tgagcacgga ttcgtcgcgc gcgagttcca ccgtcgctac
      601 cgcctgccgc ctggcgtgga tccggctgcc gtgacgtccg cgctgtcccc cgagggcgtc
      661 ctgtccatcc aggccgcacc agcgtcggcc caggccccac cgccagccgc agccaagtag
      721 gagggggctg ggccgcgccc gcaccccggg agcctcctca ggctcacgcg ccaaaatcga
      781 gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
      841 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
      901 cctttcctaa taaaatgagg a
//
