LOCUS       sequence-107352-         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA with 2x MS2 (wt+f6) for yeast cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Zalatan JG, Lee ME, Almeida R, Gilbert LA, Whitehead EH, La Russa M,
            Tsai JC, Weissman JS, Dueber JE, Qi LS, Lim WA
  TITLE     Engineering Complex Synthetic Transcriptional Programs with CRISPR 
            RNA Scaffolds.
  JOURNAL   Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052.
  PUBMED    25533786
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052."
COMMENT     Sequence Label: pJZC588
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(128..203)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 tnnnnnnnnn anncnncacn nannngacan ngctggtacc gaactctgca gaggcataca
       61 aaacaaaaaa tggnnnnnnt cccnagggac tgtgggagtc gcacatggga gatcctcatg
      121 tgctcgcgca ccgactcggt gccacttttt caagttgata acggactagn nttattttaa
      181 cttgctattt ctagctctaa aactatcagt gatagagaaa agtgatcatc gacctttcag
      241 tncgctgact tttcggaccc nnanncctgg acccctanct gatcttgggt ngnnnnnggg
      301 agttgcnttt nancancttt tcngtagtcg ancaaanncg cggcgatacc gctcccgaac
      361 cagagnncgc ctgtgattac cnttgnnntt ctccaggcca gacccnnnan antgatggag
      421 gcgtccgtan nagnggcgtg tgcgttctga cngnncagan nnggacgatt gaacttacct
      481 tcccnntcgg agccgngagt tngcnncnan cagngnacat nnnnngcntg tatgactggc
      541 tgaaaccttc cttgccctat tanagntcct gnnntcntgg tgacgcnnnn anangtnttt
      601 gtgacanagt gattcntaca nancncactc tganacanat cagaagtgaa nngcnnagga
      661 cgccacgccg ccaanagnnn nncccgatgg atacgcacga nccncattgc angnntncnn
      721 ngttacaanc acccntnngc nngnnctnag gngttttncc nggnntttnn gacgntncgn
      781 gnttgannct acccgcctna cnnccaanna ccgtttanat tngnnnatnn nnngatccng
      841 nncnnntggn ttngccnntt gtttcggngc gcaacactan gnggnaanag gncnncanan
      901 nggtttttnn ncactac
//
