LOCUS       sequence-107353-         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Cas9 in T. cruzi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Peng D, Kurup SP, Yao PY, Minning TA, Tarleton RL
  TITLE     CRISPR-Cas9-Mediated Single-Gene and Gene Family Disruption in 
            Trypanosoma cruzi.
  JOURNAL   MBio. 2014 Dec 30;6(1). pii: e02097-14. doi: 10.1128/mBio.02097-14.
  PUBMED    25550322
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2014-12-30"; volume: "6(1). pii: e02097-14. doi"; pages: " 
            10.1128/mBio.02097-14"
COMMENT     Sequence Label: pTrex-b-NLS-hSpCas9
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(311..331)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(338..403)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 agctgtcgtc caccttggcc atctcgttgc tgaagatctc ttgcagatag cagatccggt
       61 tcttccgtct ggtgtatctt cttctggcgg ttctcttcag ccgggtggcc tcggctgttt
      121 cgccgctgtc gaacagcagg gctccgatca ggttcttctt gatgctgtgc cggtcggtgt
      181 tgcccagcac cttgaatttc ttgctgggca ccttgtactc gtcggtgatc acggcccagc
      241 ccacagagtt ggtgccgatg tccaggccga tgctgtactt cttgtcggct gctgggactc
      301 cgtggatacc gaccttccgc ttcttctttg gggccatctt atcgtcatcg tctttgtaat
      361 caatatcatg atccttgtag tctccgtcgt ggtccttata gtccattcta gacaagacaa
      421 cttatagagc aaaaaaaaaa aagaaaagac atcataaaag aagttggaaa agaaaaaagg
      481 taaaaacaat ttaacacaac aataataagt ggataaaaca acaaagaaaa agtattataa
      541 taacaatcat aataatttcg ctttcgtgcg tgaaaatgtt tcttagtgaa tccgctgcat
      601 attttgaaga aactcgttgg gggatccact agaactcttg cacagacttg gcatccacac
      661 ggcgctttga ctccccttct gaaaaaaaaa gaacacgttg attccccttt tgatttccca
      721 cgaatgcgaa gcacaattca cgcacagcca acggcaggga gagacgataa ggtaaaatac
      781 acacaagtgg tggaatcaca aaaggacacg cacagcacgc aaataaacat tgcacacggc
      841 atgacatttt gcgacccaca cacaatgtgt acactacaat tatatgaa
//
