LOCUS       sequence-107357-         604 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA with 2x PP7 for yeast cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Zalatan JG, Lee ME, Almeida R, Gilbert LA, Whitehead EH, La Russa M,
            Tsai JC, Weissman JS, Dueber JE, Qi LS, Lim WA
  TITLE     Engineering Complex Synthetic Transcriptional Programs with CRISPR 
            RNA Scaffolds.
  JOURNAL   Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052.
  PUBMED    25533786
REFERENCE   2  (bases 1 to 604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052."
COMMENT     Sequence Label: pJZC603
FEATURES             Location/Qualifiers
     source          1..604
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        280..355
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     terminator      435..454
                     /label=SUP4 terminator
                     /note="transcription terminator for the S. cerevisiae SUP4 
                     tRNA gene"
     promoter        complement(483..501)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(522..538)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(522..538)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(535..557)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    546..562
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(570..576,577..594,595..600))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 gataatgtat gattatgctt tcactcatat ttatacagaa acttgatgtt ttctttcgag
       61 tatatacaag gtgattacat gtacgtttga agtacaactc tagattttgt agtgccctct
      121 tgggctagcg gtaaaggtgc gcattttttc acaccctaca atgttctgtt caaaagattt
      181 tggtcaaacg ctgtagaagt gaaagttggt gcgcatgttt cggcgttcga aacttctccg
      241 cagtgaaaga taaatgatca cttttctcta tcactgatag ttttagagct agaaatagca
      301 agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcgggag
      361 ctaaggagtt tatatggaaa cccttagcct gctgcgtaag gagtttatat ggaaaccctt
      421 acgcagcagt tccctttttt tgttttttat gtctctgcag agttcggtac cagcttttgt
      481 tccctttagt gagggttaat ttcgagcttg gcgtaatcat ggtcatagct gtttcctgtg
      541 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
      601 gcct
//
