LOCUS       sequence-107358-         595 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA with 2x PP7 for yeast cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Zalatan JG, Lee ME, Almeida R, Gilbert LA, Whitehead EH, La Russa M,
            Tsai JC, Weissman JS, Dueber JE, Qi LS, Lim WA
  TITLE     Engineering Complex Synthetic Transcriptional Programs with CRISPR 
            RNA Scaffolds.
  JOURNAL   Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052.
  PUBMED    25533786
REFERENCE   2  (bases 1 to 595)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052."
COMMENT     Sequence Label: pJZC603
FEATURES             Location/Qualifiers
     source          1..595
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(21..40)
                     /label=SUP4 terminator
                     /note="transcription terminator for the S. cerevisiae SUP4 
                     tRNA gene"
     misc_RNA        complement(120..195)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(216..484)
                     /label=SNR52 promoter
                     /note="promoter for the S. cerevisiae small nucleolar RNA 
                     gene SNR52"
     primer_bind     complement(509..528)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(510..528)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(535..552)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(535..551)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(544..566)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gctggtaccg aactctgcag agacataaaa aacaaaaaaa gggaactgct gcgtaagggt
       61 ttccatataa actccttacg cagcaggcta agggtttcca tataaactcc ttagctcccg
      121 caccgactcg gtgccacttt ttcaagttga taacggacta gccttatttt aacttgctat
      181 ttctagctct aaaactatca gtgatagaga aaagtgatca tttatctttc actgcggaga
      241 agtttcgaac gccgaaacat gcgcaccaac tttcacttct acagcgtttg accaaaatct
      301 tttgaacaga acattgtagg gtgtgaaaaa atgcgcacct ttaccgctag cccaagaggg
      361 cactacaaaa tctagagttg tacttcaaac gtacatgtaa tcaccttgta tatactcgaa
      421 agaaaacatc aagtttctgt ataaatatga gtgaaagcat aatcatacat tatcttttca
      481 aagagaattc cgagtgagct ccaattcgcc ctatagtgag tcgtattaca attcactggc
      541 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgc
//
