LOCUS       sequence-107361-        1140 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast two-hybrid bait vector expressing LexA-PPTR-1 fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Wang JT, Smith J, Chen BC, Schmidt H, Rasoloson D, Paix A, Lambrus 
            BG, Calidas D, Betzig E, Seydoux G
  TITLE     Regulation of RNA granule dynamics by phosphorylation of 
            serine-rich, intrinsically disordered proteins in C. elegans.
  JOURNAL   Elife. 2014 Dec 23;4. doi: 10.7554/eLife.04591.
  PUBMED    25535836
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014-12-23"; volume: "4. doi"; pages: " 10.7554/eLife.04591"
COMMENT     Sequence Label: pJW3.42
FEATURES             Location/Qualifiers
     source          1..1140
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(166..417,418..771)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     primer_bind     709..730
                     /label=LexA
                     /note="LexA DNA binding domain, forward primer"
ORIGIN
        1 aagtttgctg tcttgctatc aagtataaat agacctgcaa ttattaatct tttgtttcct
       61 cgtcattgtt ctcgttccct ttcttccttg tttctttttc tgcacaatat ttcaagctat
      121 accaagcata caatcaactc caagcttgaa ttaattccgg gcggaatgaa agcgttaacg
      181 gccaggcaac aagaggtgtt tgatctcatc cgtgatcaca tcagccagac aggtatgccg
      241 ccgacgcgtg cggaaatcgc gcagcgtttg gggttccgtt ccccaaacgc ggctgaagaa
      301 catctgaagg cgctggcacg caaaggcgtt attgaaattg tttccggcgc atcacgcggg
      361 attcgtctgt tgcaggaaga ggaagaaggg ttgccgctgg taggtcgtgt ggctgccggt
      421 gaaccacttc tggcgcaaca gcatattgaa ggtcattatc aggtcgatcc ttccttattc
      481 aagccgaatg ctgatttcct gctgcgcgtc agcgggatgt cgatgaaaga tatcggcatt
      541 atggatggtg acttgctggc agtgcataaa actcaggatg tacgtaacgg tcaggtcgtt
      601 gtcgcacgta ttgatgacga ggttaccgtt aagcgcctga aaaaacaggg caataaagtc
      661 gaactgttgc cagaaaatag cgagtttaaa ccaattgtcg tagatcttcg tcagcagagc
      721 ttcaccattg aagggctggc ggttggggtt attcgcaacg gcgactggct ggaattcatg
      781 cacggaagcg ggcacagtct aacgggcgct ccgcatcaaa ttccgccgcc acggacacag
      841 ggagccgcca ccggtggcca acaactctcg gcgacagcta atcaatttgt tgataaaatc
      901 gatccgttcc ataataaaag aggaacttct agacgattgc gcatcaacaa cagcagccga
      961 tataatgttg attcggctca agaactcgta caattggcgc tcatcaaaga tactgctgca
     1021 aatgagcaac cggctctcgt tatcgaaaag ctcgtccaat gtcagcacgt attcgatttc
     1081 tatgatccag tagctcaact gaaatgcaaa gaaatcaaaa gagcagctct aaatgagctc
//
