LOCUS       sequence-107366-         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of HSPB6  (small HSP) tagged with V5 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Vos MJ, Zijlstra MP, Kanon B, van Waarde-Verhagen MA, Brunt ER, 
            Oosterveld-Hut HM, Carra S, Sibon OC, Kampinga HH
  TITLE     HSPB7 is the most potent polyQ aggregation suppressor within the 
            HSPB family of molecular chaperones.
  JOURNAL   Hum Mol Genet. 2010 Dec 1;19(23):4677-93. doi: 10.1093/hmg/ddq398. 
            Epub 2010 Sep 15.
  PUBMED    20843828
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/hmg/ddq398"; journalName: "Hum Mol Genet"; date: 
            "2010-12-1- 1"; volume: "19"; issue: "23"; pages: "4677-93"
COMMENT     Sequence Label: Frt-V5-hspB6
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(563..604)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(707..731)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    741..759
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    762..780
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 gattttggcg cgtgagcctg aggaggctcc cggggtgcgg gcgcggccca gccccctcct
       61 acttggctgc ggctggcggt ggggcctggg ccgacgctgg tgcggcctgg atggacagga
      121 cgccctcggg ggacagcgcg gacgtcacgg cagccggatc cacgccaggc ggcaggcggt
      181 agcgacggtg gaactcgcgc gcgacgaatc cgtgctcatc cgggcgctcc tcgtggcgcg
      241 cgtgcacctc cacgtgttcg cccaccacct tgacagcaat ttcctccggc gagaagtgct
      301 tcacgtctag cagcaccgaa aagtggccgg ggtccgtcgg cacctgggcg acgggcagcg
      361 ccacgctggg tgcgcgcagg tagtaggggg cgagcgtggt ggggcagagc gcagccagct
      421 cggcctccag cagcccctcg ccgaagcgct ggtcaaagag gcgtccgggc gccgaaagtc
      481 cgggcaacgg ggccgaggcg cggcgcagcc aagacggctg cacaggcaca gggatctcca
      541 tggttctcga ggttttggat cccgtagaat cgagaccgag gagagggtta gggataggct
      601 tacccatggt gaattcaagc ttaagtttaa acgctagagt ccggaggctg gatcggtccc
      661 ggtgtcttct atggaggtca aaacagcgtg gatggcgtct ccaggcgatc tgacggttca
      721 ctaaacgagc tcgtcgacga tctctatcac tgatagggag atctctatca ctgataggga
      781 gagctctgct tatata
//
