LOCUS       sequence-107370-        1099 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast two-hybrid bait vector expressing LexA-PPTR-1 fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Wang JT, Smith J, Chen BC, Schmidt H, Rasoloson D, Paix A, Lambrus 
            BG, Calidas D, Betzig E, Seydoux G
  TITLE     Regulation of RNA granule dynamics by phosphorylation of 
            serine-rich, intrinsically disordered proteins in C. elegans.
  JOURNAL   Elife. 2014 Dec 23;4. doi: 10.7554/eLife.04591.
  PUBMED    25535836
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014-12-23"; volume: "4. doi"; pages: " 10.7554/eLife.04591"
COMMENT     Sequence Label: pJW3.42
FEATURES             Location/Qualifiers
     source          1..1099
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    95..116
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(131..136,137..154,155..161)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    169..185
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     174..196
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     193..209
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     193..209
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        223..324
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             325..999
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
ORIGIN
        1 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
       61 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
      121 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
      181 aacaatttca cacaggaaac agctatgaca tgattacgaa ttaattcggt cgaaaaaaga
      241 aaaggagagg gccaagaggg agggcattgg tgactattga gcacgtgagt atacgtgatt
      301 aagcacacaa aggcagcttg gagtatgtct gttattaatt tcacaggtag ttctggtcca
      361 ttggtgaaag tttgcggctt gcagagcaca gaggccgcag aatgtgctct agattccgat
      421 gctgacttgc tgggtattat atgtgtgccc aatagaaaga gaacaattga cccggttatt
      481 gcaaggaaaa tttcaagtct tgtaaaagca tataaaaata gttcaggcac tccgaaatac
      541 ttggttggcg tgtttcgtaa tcaacctaag gaggatgttt tggctctggt caatgattac
      601 ggcattgata tcgtccaact gcatggagat gagtcgtggc aagaatacca agagttcctc
      661 ggtttgccag ttattaaaag actcgtattt ccaaaagact gcaacatact actcagtgca
      721 gcttcacaga aacctcattc gtttattccc ttgtttgatt cagaagcagg tgggacaggt
      781 gaacttttgg attggaactc gatttctgac tgggttggaa ggcaagagag ccccgaaagc
      841 ttacatttta tgttagctgg tggactgacg ccagaaaatg ttggtgatgc gcttagatta
      901 aatggcgtta ttggtgttga tgtaagcgga ggtgtggaga caaatggtgt aaaagactct
      961 aacaaaatag caaatttcgt caaaaatgct aagaaatagg ttattactga gtagtattta
     1021 tttaagtatt gtttgtgcac ttgccggcat gccggtagag gtgtggtcaa taagagcgac
     1081 ctcatgctat acctgagaa
//
