LOCUS       sequence-107371-         694 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses MCP-VP64 and dCas9 in Yeast cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Zalatan JG, Lee ME, Almeida R, Gilbert LA, Whitehead EH, La Russa M,
            Tsai JC, Weissman JS, Dueber JE, Qi LS, Lim WA
  TITLE     Engineering Complex Synthetic Transcriptional Programs with CRISPR 
            RNA Scaffolds.
  JOURNAL   Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052.
  PUBMED    25533786
REFERENCE   2  (bases 1 to 694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052."
COMMENT     Sequence Label: pJZC595
FEATURES             Location/Qualifiers
     source          1..694
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             138..158
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             531..680
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
ORIGIN
        1 tagacctgca attattaatc ttttgtttcc tcgtcattgt tctcgttccc tttcttcctt
       61 gtttcttttt ctgcacaata tttcaagcta taccaagcat acaatcaact atctcatata
      121 caatgtctct cgagatgccc aaaaagaaaa gaaaagtggg tagtatggct tctaacttta
      181 ctcagttcgt tctcgtcgac aatggcggaa ctggcgacgt gactgtcgcc ccaagcaact
      241 tcgctaacgg gatcgctgaa tggatcagct ctaactcgcg ttcacaggct tacaaagtaa
      301 cctgtagcgt tcgtcagagc tctgcgcaga atcgcaaata caccatcaaa gtcgaggtgc
      361 ctaaaggcgc ctggcgttcg tacttaaata tggaactaac cattccaatt ttcgccacga
      421 attccgactg cgagcttatt gttaaggcaa tgcaaggtct cctaaaagat ggaaacccga
      481 ttccctcagc aatcgcagca aactccggca tctacggctc cggacgagcc gatgctctcg
      541 acgattttga cctcgatatg ctgggatccg acgctctcga tgacttcgac cttgacatgc
      601 tggggagtga cgctctggac gacttcgatc tcgacatgct gggctccgac gccctggatg
      661 acttcgatct ggacatgctc atcaactgaa gtgc
//
