LOCUS       sequence-107373-         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses MCP-VP64 and dCas9 in Yeast cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Zalatan JG, Lee ME, Almeida R, Gilbert LA, Whitehead EH, La Russa M,
            Tsai JC, Weissman JS, Dueber JE, Qi LS, Lim WA
  TITLE     Engineering Complex Synthetic Transcriptional Programs with CRISPR 
            RNA Scaffolds.
  JOURNAL   Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052.
  PUBMED    25533786
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052."
COMMENT     Sequence Label: pJZC595
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(145..165)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(169..189)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(193..213)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tcattttata gagtatatac aactgtataa gatagtaata aaaatatcgc actcacgtaa
       61 acacttaatc ataatattaa ttcgtatata atttagctat ttgcttagag ctccaccgcg
      121 gtggcggccg ccggtagaat tctatacctt tctcttcttt tttggatcta cctttctctt
      181 cttttttgga tctacctttc tcttcttttt tggatcagct ccctcatccc ctccgagctg
      241 tgagaggtca atccgagttt cgtaaagtcc ggtgatgctc tggtggatga gggtggcgtc
      301 cagcacttct ttggtggagg tatagcgttt ccggtcgatg gtggtgtcga agtacttgaa
      361 tgcggcaggg gcaccaagat tggtgagggt gaacaggtgg atgatattct cagcctgctc
      421 ccgaattggt ttgtctctgt gcttattgta tgcgctaagg actttatcga ggttggcatc
      481 agcgagaatg acgcgtttag aaaactcgga aatctgctca atgatctcgt ccagataatg
      541 cttatgctgc tccacaaaca gttgcttttg ctcgttgtcc tcggggctcc ccttcagctt
      601 ctcgtaatgg cttgcaaggt agaggaaatt cacgtacttg gaggggagag ccagctcatt
      661 acccttctga agttcgccag cggaggcgag catccgcttc cgcccattct ccagttcgaa
      721 caggctatac tttgggagct tgatgat
//
