LOCUS       sequence-107374-         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Cas9 in T. cruzi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Peng D, Kurup SP, Yao PY, Minning TA, Tarleton RL
  TITLE     CRISPR-Cas9-Mediated Single-Gene and Gene Family Disruption in 
            Trypanosoma cruzi.
  JOURNAL   MBio. 2014 Dec 30;6(1). pii: e02097-14. doi: 10.1128/mBio.02097-14.
  PUBMED    25550322
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2014-12-30"; volume: "6(1). pii: e02097-14. doi"; pages: " 
            10.1128/mBio.02097-14"
COMMENT     Sequence Label: pTrex-b-NLS-hSpCas9
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             273..320
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 agagagtgat cctggccgac gctaatctgg acaaagtgct gtccgcctac aacaagcacc
       61 gggataagcc catcagagag caggccgaga atatcatcca cctgtttacc ctgaccaatc
      121 tgggagcccc tgccgccttc aagtactttg acaccaccat cgaccggaag aggtacacca
      181 gcaccaaaga ggtgctggac gccaccctga tccaccagag catcaccggc ctgtacgaga
      241 cacggatcga cctgtctcag ctgggaggcg acaaaaggcc ggcggccacg aaaaaggccg
      301 gccaggcaaa aaagaaaaag taactcgagc catttacgac tccaaggcaa cgctgcagaa
      361 caacctgccg aaggagcgcc gcttcttcaa gattgtgtcg tggtacgaca acgagtgggg
      421 atactcccac cgcgtggtgg accttgtacg ccacatggcc tcgaaggatc gttcggcaag
      481 gttgtaggcg tggcgatgac ttcaggtctt tcttttgcga atagggatct tataatacac
      541 gatgcgtgtc ccgtgatgat cgttaccggt gctgccacga tccaattgac acagcgtcaa
      601 gagcaaaaca attttacttt tccctttaag gacaacaaca aaaaaatata taactttttt
      661 ttcttttttt ttttttttga aattatattt atggtcatct ttgggaaaca aaaagcagca
      721 atttaatgat gcggaaggat gagtgaaata atgtttaatc aatgtacgag gatttggggt
      781 attgcaagga a
//
