LOCUS       sequence-107381-         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of MBP-MEG-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Wang JT, Smith J, Chen BC, Schmidt H, Rasoloson D, Paix A, Lambrus 
            BG, Calidas D, Betzig E, Seydoux G
  TITLE     Regulation of RNA granule dynamics by phosphorylation of 
            serine-rich, intrinsically disordered proteins in C. elegans.
  JOURNAL   Elife. 2014 Dec 23;4. doi: 10.7554/eLife.04591.
  PUBMED    25535836
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014-12-23"; volume: "4. doi"; pages: " 10.7554/eLife.04591"
COMMENT     Sequence Label: pJW4.21
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..45
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    49..73
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aacaacaaca ataacaataa caacaacctc gggatcgagg gaaggatcac aagtttgtac
       61 aaaaaagcag gcttaatgga taatcgtggt catttttctt caaatgggaa tttcccaccg
      121 caaggatatc accgacgtga gcagtcccaa gaaggaatga gaatcgggaa tcatcacgga
      181 ccgttttctt cgggcaacat gcgtagtatt ggaggctcag ctcaaaacca gcagcaacgt
      241 cattggacaa acatgctcaa tggctcaaat aatgttgaga attcttgggt gtgcttctct
      301 ggaaacacgt ctcttgccga tgatccaaat gttctctcca atttctctgc gttggcacaa
      361 caaagagtta accattttga tacaattgtg caggaaaggg acaataggaa tgcgagtttc
      421 ctgaatggtt cagcagttgg aaataacctg aacacttcgt ttagcgtttt tggcaacttg
      481 cgaggaggag accaagatca tcgagttctt tccgatcaca ctggaattta cactggtttg
      541 ggatccactg gacaaaacgc tgttggaacc gggataagac tggccgctga tgtcgcgaac
      601 ggcaatttct tggagcagag agcacctact gccatgggac acaaccaaag ctattctgca
      661 ctgaaccagt cattagcacc aacactcctt gaccaataca atcaggcact cctcgaccaa
      721 tacaatcagt cttcacaaat ggctcaagga cgaggatatc cggctccaaa tatagcttac
      781 ggtcttcaaa atgccggttt tccagctcca caaatagctc atcggccgaa tactcaaaat
      841 gcagatcctc aagcgatgaa tatga
//
