LOCUS       sequence-107393-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes a PI(4,5)P2 lipid selective PH domain that can be used as a 
            fluorescent translocation biosensor to monitor changes or local 
            differences in the concentration of plasma membrane PI(4,5)P2 
            lipids.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Stauffer TP, Ahn S, Meyer T
  TITLE     Receptor-induced transient reduction in plasma membrane 
            PtdIns(4,5)P2 concentration monitored in living cells.
  JOURNAL   Curr Biol. 1998 Mar 12. 8(6):343-6.
  PUBMED    9512420
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            1998 Mar 12. 8(6):343-6."
COMMENT     Sequence Label: GFP-C1-PLCdelta-PH
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    645..710
                     /label=MCS
                     /note="multiple cloning site"
     primer_bind     complement(753..774)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
       61 aaagcaagta aaacctctac aaatgtggta tggctgatta tgatcagtta tctagattac
      121 tggatgttga gctccttcag gaagttctgc agctccttga agctcatctt gttgtccttg
      181 tttttgtcag cttttcgcaa gcaggagtga atccagtgct gtagcttctg acgctggtcc
      241 atggagcctg agtggtggat gatcttgtgc agccccagca cccagtgctg ggcatcagct
      301 ggcgatgggg cgatgaggtc tagtgtattg cgctggtcct tgaagacaat ggagaagcag
      361 cggtcctcgg gcacatcacg ggcgaacttc tccagaccct ccgtgcggtg ccccattcgc
      421 acctcctgaa tgtcctcgat ggagaacagc tgggactccg gggtccgcat gaccttgcgg
      481 gactcctgcc agatggtctt gcagtcctcc tgcaacttgt agaagcgctc tctcctccat
      541 gagctggact tcaccttcag gagctggctg cccttcagca gcgcctgtag atcctcatca
      601 tcctgtaggc cgtgcagggt caggaagtcc cggcccgagt ccatggatcc cgggcccgcg
      661 gtaccgtcga ctgcagaatt cgaagcttga gctcgagatc tgagtccgga cttgtacagc
      721 tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac catgtgatcg
      781 cgcttctcgt tggggtcttt gctcagggcg gactgggtgc tcaggtagtg gttgtcgggc
      841 agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc gagctgcacg
//
