LOCUS       sequence-107395-         535 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA + 1x PP7 with mCherry for mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Zalatan JG, Lee ME, Almeida R, Gilbert LA, Whitehead EH, La Russa M,
            Tsai JC, Weissman JS, Dueber JE, Qi LS, Lim WA
  TITLE     Engineering Complex Synthetic Transcriptional Programs with CRISPR 
            RNA Scaffolds.
  JOURNAL   Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052.
  PUBMED    25533786
REFERENCE   2  (bases 1 to 535)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052."
COMMENT     Sequence Label: pJZC39
FEATURES             Location/Qualifiers
     source          1..535
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    67..100
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(398..417)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 agaggttgat tatcgataag cttgatatcg aattgtacct agtggaaccg gaacccttaa
       61 acatgtataa cttcgtataa tgtatgctat acgaagttat taggtccctc gacgaattct
      121 tacttgtaca gctcgtccat gccgccggtg gagtggcggc cctcggcgcg ttcgtactgt
      181 tccacgatgg tgtagtcctc gttgtgggag gtgatgtcca acttgatgtt gacgttgtag
      241 gcgccgggca gctgcacggg cttcttggcc ttgtaggtgg tcttgacctc agcgtcgtag
      301 tggccgccgt ccttcagctt cagcctctgc ttgatctcgc ccttcagggc gccgtcctcg
      361 gggtacatcc gctcggagga ggcctcccag cccatggtct tcttctgcat tacggggccg
      421 tcggagggga agttggtgcc gcgcagcttc accttgtaga tgaactcgcc gtcctgcagg
      481 gaggagtcct gggtcacggt caccacgccg ccgtcctcga agttcatcac gcgct
//
