LOCUS       sequence-107400-         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of MBP-MEG-3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Wang JT, Smith J, Chen BC, Schmidt H, Rasoloson D, Paix A, Lambrus 
            BG, Calidas D, Betzig E, Seydoux G
  TITLE     Regulation of RNA granule dynamics by phosphorylation of 
            serine-rich, intrinsically disordered proteins in C. elegans.
  JOURNAL   Elife. 2014 Dec 23;4. doi: 10.7554/eLife.04591.
  PUBMED    25535836
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014-12-23"; volume: "4. doi"; pages: " 10.7554/eLife.04591"
COMMENT     Sequence Label: pJW4.23
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             38..49
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    53..77
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gaacaacaac aacaataaca ataacaacaa cctcgggatc gagggaagga tcacaagttt
       61 gtacaaaaaa gcaggcttaa tgagttcctc aaaaccttac ccaagcggtc tgccaaactc
      121 gagaagaaaa agaggaggtc gccgctcaag tagtcgtagc aatcaggaaa gtgcatccaa
      181 caacatggaa caccaaatca ctcttgacga attgttcaat ccgatcgcaa aacaagacag
      241 tgctcagtca acaagccgtg aatatggtgc aaaatcgggg atatctcatc acggatccgt
      301 atcattcaac gggaatacat tcatgaatgg ccaacagctg aatcactcga tgactcgaca
      361 tggtcgagta ttcaatcagt cgatgcatgc agctcaagga aacggcagca acgcctttaa
      421 cagtattcct cctacggccc ccgtcttctc tgcagacttc cgccgcaact tgcaaacacg
      481 caatagctcc agctggtatg aacgaagatt cccagtatcc acagatcagg acgacgttca
      541 gcaatcaaac acaagacgta gtagaagtcg tcagaatgga caacatggcc taagcttctc
      601 ggatggttcg aacaattatg gtcacgctgg gaataagtca ttcagtgtga gttccgttcc
      661 cgttgggttc cagaagcaag aaaataattc taagaaactg cgacaaacca atgttcacca
      721 gcaatgtctt ggaaataaaa gcttcaacgc tcaagctgga gttcatggac acgcttttaa
      781 aaaaggccat aaggacaata aaaatgcatc tggaaaggag gttatcaatt ctagtcttgt
      841 ccaaaagcac gatgctatta agtcgcggaa tttgaaccag agcttctctg gatttcctac
      901 acatgaaacc tc
//
