LOCUS       sequence-107402-         650 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA + 2x PP7 with mCherry for mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Zalatan JG, Lee ME, Almeida R, Gilbert LA, Whitehead EH, La Russa M,
            Tsai JC, Weissman JS, Dueber JE, Qi LS, Lim WA
  TITLE     Engineering Complex Synthetic Transcriptional Programs with CRISPR 
            RNA Scaffolds.
  JOURNAL   Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052.
  PUBMED    25533786
REFERENCE   2  (bases 1 to 650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052."
COMMENT     Sequence Label: pJZC40
FEATURES             Location/Qualifiers
     source          1..650
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    69..102
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(400..419)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ccagaggttg attatcgata agcttgatat cgaattgtac ctagtggaac cggaaccctt
       61 aaacatgtat aacttcgtat aatgtatgct atacgaagtt attaggtccc tcgacgaatt
      121 cttacttgta cagctcgtcc atgccgccgg tggagtggcg gccctcggcg cgttcgtact
      181 gttccacgat ggtgtagtcc tcgttgtggg aggtgatgtc caacttgatg ttgacgttgt
      241 aggcgccggg cagctgcacg ggcttcttgg ccttgtaggt ggtcttgacc tcagcgtcgt
      301 agtggccgcc gtccttcagc ttcagcctct gcttgatctc gcccttcagg gcgccgtcct
      361 cggggtacat ccgctcggag gaggcctccc agcccatggt cttcttctgc attacggggc
      421 cgtcggaggg gaagttggtg ccgcgcagct tcaccttgta gatgaactcg ccgtcctgca
      481 gggaggagtc ctgggtcacg gtcaccacgc cgccgtcctc gaagttcatc acgcgctccc
      541 acttgaagcc ctcggggaag gacagcttca agtagtcggg gatgtcggcg gggtgcttca
      601 cgtaggcctt ggagccgtac atgaactgag gggacaggat gtcccaggcg
//
