LOCUS       sequence-107423-       12122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Nm dCas9-3XGFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12122)
  AUTHORS   Ma H, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T
  TITLE     Multicolor CRISPR labeling of chromosomal loci in human cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 
            10.1073/pnas.1420024112. Epub 2015 Feb 23.
  PUBMED    25713381
REFERENCE   2  (bases 1 to 12122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1420024112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-03-10- 10"; volume: "112"; issue: "10"; pages: "3002-7"
COMMENT     Sequence Label: pHAGE-TO-nmdCas9-3XGFP
FEATURES             Location/Qualifiers
     source          1..12122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2299..2602
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2603..2806
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2757..2777
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2808..2826
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2829..2847
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2857..2881
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2974..2983
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2986..3006
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(3019..3060,3061..3063,3064..4773,4774..4779,4780..4845,
                     4846..4848,4849..6261)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Neisseria meningitidis Type II 
                     CRISPR/Cas system"
                     /label=NmdCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D16A, D587A, 
                     H588A, and N611A"
                     /translation="AAFKPNPINYILGLAIGIASVGWAMVEIDEDENPICLIDLGVRVF
                     ERAEVPKTGDSLAMARRLARSVRRLTRRRAHRLLRARRLLKREGVLQAADFDENGLIKS
                     LPNTPWQLRAAALDRKLTPLEWSAVLLHLIKHRGYLSQRKNEGETADKELGALLKGVAD
                     NAHALQTGDFRTPAELALNKFEKESGHIRNQRGDYSHTFSRKDLQAELILLFEKQKEFG
                     NPHVSGGLKEGIETLLMTQRPALSGDAVQKMLGHCTFEPAEPKAAKNTYTAERFIWLTK
                     LNNLRILEQGSERPLTDTERATLMDEPYRKSKLTYAQARKLLGLEDTAFFKGLRYGKDN
                     AEASTLMEMKAYHAISRALEKEGLKDKKSPLNLSPELQDEIGTAFSLFKTDEDITGRLK
                     DRIQPEILEALLKHISFDKFVQISLKALRRIVPLMEQGKRYDEACAEIYGDHYGKKNTE
                     EKIYLPPIPADEIRNPVVLRALSQARKVINGVVRRYGSPARIHIETAREVGKSFKDRKE
                     IEKRQEENRKDREKAAAKFREYFPNFVGEPKSKDILKLRLYEQQHGKCLYSGKEINLGR
                     LNEKGYVEIAAALPFSRTWDDSFNNKVLVLGSEAQNKGNQTPYEYFNGKDNSREWQEFK
                     ARVETSRFPRSKKQRILLQKFDEDGFKERNLNDTRYVNRFLCQFVADRMRLTGKGKKRV
                     FASNGQITNLLRGFWGLRKVRAENDRHHALDAVVVACSTVAMQQKITRFVRYKEMNAFD
                     GKTIDKETGEVLHQKTHFPQPWEFFAQEVMIRVFGKPDGKPEFEEADTPEKLRTLLAEK
                     LSSRPEAVHEYVTPLFVSRAPNRKMSGQGHMETVKSAKRLDEGVSVLRVPLTQLKLKDL
                     EKMVNREREPKLYEALKARLEAHKDDPAKAFAEPFYKYDKAGNRTQQVKAVRVEQVQKT
                     GVWVRNHNGIADNATMVRVDVFEKGDKYYLVPIYSWQVAKGILPDRAVVQGKDEEDWQL
                     IDDSFNFKFSLHPNDLVEVITKKARMFGYFASCHRGTGNINIRIHDLDHKIGKNGILEG
                     IGVKTALSFQKYQIDELGKEIRPCRLKKRPPVR"
     regulatory      5848..5857
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6280..6300
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6313..7026
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             7045..7758
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             7777..8490
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     misc_feature    8512..9100
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8565..8585)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8983..8994)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9175..9408
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9527..9545)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9613..9717
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9718..9786,9787..10578)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9936..9955)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10749..11337
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11238..11257
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11491..11508
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11748..11769
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11784..11789,11790..11807,11808..11814)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11822..11838
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11827..11849
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11846..11862
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11846..11862
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11912..11931
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttgacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagcgat
     2221 gctagctgat gcgggcccta gttattaata gtaatcaatt acggggtcat tagttcatag
     2281 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     2341 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     2401 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     2461 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     2521 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     2581 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
     2641 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     2701 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     2761 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctctcc ctatcagtga
     2821 tagagatctc cctatcagtg atagagatcg tcgacgagct cgtttagtga accgtcagat
     2881 cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag
     2941 cctaccggtt aaggtgaacg cgtgcaggct ggcgccacca tggcccctaa aaagaaacgc
     3001 aaggtcgaat tcggaagcgc cgccttcaag cccaacccca tcaactacat cctgggcctg
     3061 gccatcggca tcgccagcgt gggctgggcc atggtggaga tcgacgagga cgagaacccc
     3121 atctgcctga tcgacctggg tgtgcgcgtg ttcgagcgcg ctgaggtgcc caagactggt
     3181 gacagtctgg ctatggctcg ccggcttgct cgctctgttc ggcgccttac tcgccggcgc
     3241 gctcaccgcc ttctgcgcgc tcgccgcctg ctgaagcgcg agggtgtgct gcaggctgcc
     3301 gacttcgacg agaacggcct gatcaagagc ctgcccaaca ctccttggca gctgcgcgct
     3361 gccgctctgg accgcaagct gactcctctg gagtggagcg ccgtgctgct gcacctgatc
     3421 aagcaccgcg gctacctgag ccagcgcaag aacgagggcg agaccgccga caaggagctg
     3481 ggtgctctgc tgaagggcgt ggccgacaac gcccacgccc tgcagactgg tgacttccgc
     3541 actcctgctg agctggccct gaacaagttc gagaaggaga gcggccacat ccgcaaccag
     3601 cgcggcgact acagccacac cttcagccgc aaggacctgc aggccgagct gatcctgctg
     3661 ttcgagaagc agaaggagtt cggcaacccc cacgtgagcg gcggcctgaa ggagggcatc
     3721 gagaccctgc tgatgaccca gcgccccgcc ctgagcggcg acgccgtgca gaagatgctg
     3781 ggccactgca ccttcgagcc agccgagccc aaggccgcca agaacaccta caccgccgag
     3841 cgcttcatct ggctgaccaa gctgaacaac ctgcgcatcc tggagcaggg cagcgagcgc
     3901 cccctgaccg acaccgagcg cgccaccctg atggacgagc cctaccgcaa gagcaagctg
     3961 acctacgccc aggcccgcaa gctgctgggt ctggaggaca ccgccttctt caagggcctg
     4021 cgctacggca aggacaacgc cgaggccagc accctgatgg agatgaaggc ctaccacgcc
     4081 atcagccgcg ccctggagaa ggagggcctg aaggacaaga agagtcctct gaacctgagc
     4141 cccgagctgc aggacgagat cggcaccgcc ttcagcctgt tcaagaccga cgaggacatc
     4201 accggccgcc tgaaggaccg catccagccc gagatcctgg aggccctgct gaagcacatc
     4261 agcttcgaca agttcgtgca gatcagcctg aaggccctgc gccgcatcgt gcccctgatg
     4321 gagcagggca agcgctacga cgaggcctgc gccgagatct acggcgacca ctacggcaag
     4381 aagaacaccg aggagaagat ctacctgcct cctatccccg ccgacgagat ccgcaacccc
     4441 gtggtgctgc gcgccctgag ccaggcccgc aaggtgatca acggcgtggt gcgccgctac
     4501 ggcagccccg cccgcatcca catcgagacc gcccgcgagg tgggcaagag cttcaaggac
     4561 cgcaaggaga tcgagaagcg ccaggaggag aaccgcaagg accgcgagaa ggccgccgcc
     4621 aagttccgcg agtacttccc caacttcgtg ggcgagccca agagcaagga catcctgaag
     4681 ctgcgcctgt acgagcagca gcacggcaag tgcctgtaca gcggcaagga gatcaacctg
     4741 ggccgcctga acgagaaggg ctacgtggag atcgccgctg ccctgccctt cagccgcacc
     4801 tgggacgaca gcttcaacaa caaggtgctg gtgctgggca gcgaggctca gaacaagggc
     4861 aaccagaccc cctacgagta cttcaacggc aaggacaaca gccgcgagtg gcaggagttc
     4921 aaggcccgcg tggagaccag ccgcttcccc cgcagcaaga agcagcgcat cctgctgcag
     4981 aagttcgacg aggacggctt caaggagcgc aacctgaacg acacccgcta cgtgaaccgc
     5041 ttcctgtgcc agttcgtggc cgaccgcatg cgcctgaccg gcaagggcaa gaagcgcgtg
     5101 ttcgccagca acggccagat caccaacctg ctgcgcggct tctggggcct gcgcaaggtg
     5161 cgcgccgaga acgaccgcca ccacgccctg gacgccgtgg tggtggcctg cagcaccgtg
     5221 gccatgcagc agaagatcac ccgcttcgtg cgctacaagg agatgaacgc cttcgacggt
     5281 aaaaccatcg acaaggagac cggcgaggtg ctgcaccaga agacccactt cccccagccc
     5341 tgggagttct tcgcccagga ggtgatgatc cgcgtgttcg gcaagcccga cggcaagccc
     5401 gagttcgagg aggccgacac ccccgagaag ctgcgcaccc tgctggccga gaagctgagc
     5461 agccgccctg aggccgtgca cgagtacgtg actcctctgt tcgtgagccg cgcccccaac
     5521 cgcaagatga gcggtcaggg tcacatggag accgtgaaga gcgccaagcg cctggacgag
     5581 ggcgtgagcg tgctgcgcgt gcccctgacc cagctgaagc tgaaggacct ggagaagatg
     5641 gtgaaccgcg agcgcgagcc caagctgtac gaggccctga aggcccgcct ggaggcccac
     5701 aaggacgacc ccgccaaggc cttcgccgag cccttctaca agtacgacaa ggccggcaac
     5761 cgcacccagc aggtgaaggc cgtgcgcgtg gagcaggtgc agaagaccgg cgtgtgggtg
     5821 cgcaaccaca acggcatcgc cgacaacgcc accatggtgc gcgtggacgt gttcgagaag
     5881 ggcgacaagt actacctggt gcccatctac agctggcagg tggccaaggg catcctgccc
     5941 gaccgcgccg tggtgcaggg caaggacgag gaggactggc agctgatcga cgacagcttc
     6001 aacttcaagt tcagcctgca ccccaacgac ctggtggagg tgatcaccaa gaaggcccgc
     6061 atgttcggct acttcgccag ctgccaccgc ggcaccggca acatcaacat ccgcatccac
     6121 gacctggacc acaagatcgg caagaacggc atcctggagg gcatcggcgt gaagaccgcc
     6181 ctgagcttcc agaagtacca gatcgacgag ctgggcaagg agatccgccc ctgccgcctg
     6241 aagaagcgcc ctcctgtgcg cggctctact agtggctctc ccaagaagaa gaggaaggta
     6301 ggatccggaa gtatgcgtaa aggcgaagag ctgttcactg gtgtcgtccc tattctggtg
     6361 gaactggatg gtgatgtcaa cggtcataag ttttccgtgc gtggcgaggg tgaaggtgac
     6421 gcaactaatg gtaaactgac gctgaagttc atctgtacta ctggtaaact gccggtacct
     6481 tggccgactc tggtaacgac gctgacttat ggtgttcagt gctttgctcg ttatccggac
     6541 catatgaagc agcatgactt cttcaagtcc gccatgccgg aaggctatgt gcaggaacgc
     6601 acgatttcct ttaaggatga cggcacgtac aaaacgcgtg cggaagtgaa atttgaaggc
     6661 gataccctgg taaaccgcat tgagctgaaa ggcattgact ttaaagaaga cggcaatatc
     6721 ctgggccata agctggaata caattttaac agccacaatg tttacatcac cgccgataaa
     6781 caaaaaaatg gcattaaagc gaattttaaa attcgccaca acgtggagga tggcagcgtg
     6841 cagctggctg atcactacca gcaaaacact ccaatcggtg atggtcctgt tctgctgcca
     6901 gacaatcact atctgagcac gcaaagcgtt ctgtctaaag atccgaacga gaaacgcgat
     6961 catatggttc tgctggagtt cgtaaccgca gcgggcatca cgcatggtat ggatgaactg
     7021 tacaaatctg gcggccgctc tggcatgcgt aaaggcgaag agctgttcac tggtgtcgtc
     7081 cctattctgg tggaactgga tggtgatgtc aacggtcata agttttccgt gcgtggcgag
     7141 ggtgaaggtg acgcaactaa tggtaaactg acgctgaagt tcatctgtac tactggtaaa
     7201 ctgccggtac cttggccgac tctggtaacg acgctgactt atggtgttca gtgctttgct
     7261 cgttatccgg accatatgaa gcagcatgac ttcttcaagt ccgccatgcc ggaaggctat
     7321 gtgcaggaac gcacgatttc ctttaaggat gacggcacgt acaaaacgcg tgcggaagtg
     7381 aaatttgaag gcgataccct ggtaaaccgc attgagctga aaggcattga ctttaaagaa
     7441 gacggcaata tcctgggcca taagctggaa tacaatttta acagccacaa tgtttacatc
     7501 accgccgata aacaaaaaaa tggcattaaa gcgaatttta aaattcgcca caacgtggag
     7561 gatggcagcg tgcagctggc tgatcactac cagcaaaaca ctccaatcgg tgatggtcct
     7621 gttctgctgc cagacaatca ctatctgagc acgcaaagcg ttctgtctaa agatccgaac
     7681 gagaaacgcg atcatatggt tctgctggag ttcgtaaccg cagcgggcat cacgcatggt
     7741 atggatgaac tgtacaaagg tagcatcgat ggtagcatgc gtaaaggcga agagctgttc
     7801 actggtgtcg tccctattct ggtggaactg gatggtgatg tcaacggtca taagttttcc
     7861 gtgcgtggcg agggtgaagg tgacgcaact aatggtaaac tgacgctgaa gttcatctgt
     7921 actactggta aactgccggt accttggccg actctggtaa cgacgctgac ttatggtgtt
     7981 cagtgctttg ctcgttatcc ggaccatatg aagcagcatg acttcttcaa gtccgccatg
     8041 ccggaaggct atgtgcagga acgcacgatt tcctttaagg atgacggcac gtacaaaacg
     8101 cgtgcggaag tgaaatttga aggcgatacc ctggtaaacc gcattgagct gaaaggcatt
     8161 gactttaaag aagacggcaa tatcctgggc cataagctgg aatacaattt taacagccac
     8221 aatgtttaca tcaccgccga taaacaaaaa aatggcatta aagcgaattt taaaattcgc
     8281 cacaacgtgg aggatggcag cgtgcagctg gctgatcact accagcaaaa cactccaatc
     8341 ggtgatggtc ctgttctgct gccagacaat cactatctga gcacgcaaag cgttctgtct
     8401 aaagatccga acgagaaacg cgatcatatg gttctgctgg agttcgtaac cgcagcgggc
     8461 atcacgcatg gtatggatga actgtacaaa ctcgagtagg gtaatctaga taatcaacct
     8521 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     8581 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     8641 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     8701 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     8761 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     8821 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     8881 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     8941 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     9001 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     9061 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctgagatcct ttaagaccaa
     9121 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     9181 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     9241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     9301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     9361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
     9421 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc
     9481 ccgggttaat taaggaaagg gctagatcat tcttgaagac gaaagggcct cgtgatacgc
     9541 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     9601 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     9661 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     9721 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     9781 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     9841 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     9901 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     9961 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
    10021 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
    10081 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
    10141 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
    10201 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
    10261 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
    10321 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
    10381 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
    10441 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
    10501 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
    10561 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
    10621 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
    10681 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
    10741 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
    10801 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
    10861 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
    10921 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
    10981 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
    11041 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
    11101 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
    11161 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
    11221 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
    11281 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
    11341 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
    11401 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
    11461 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
    11521 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagcaagctc
    11581 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
    11641 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctccccgtgg
    11701 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
    11761 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
    11821 attgtgagcg gataacaatt tcacacagga aacagctatg acatgattac gaatttcaca
    11881 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
    11941 tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc
    12001 ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc
    12061 tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta
    12121 gt
//
