LOCUS       sequence-107450-         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice 
            ubiquitin promoter for transient expression in protoplast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Xie K, Minkenberg B, Yang Y
  TITLE     Boosting CRISPR/Cas9 multiplex editing capability with the 
            endogenous tRNA-processing system.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Mar 17;112(11):3570-5. doi: 
            10.1073/pnas.1420294112. Epub 2015 Mar 2.
  PUBMED    25733849
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1420294112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-03-17- 17"; volume: "112"; issue: "11"; pages: "3570-5"
COMMENT     Sequence Label: pRGE32
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        386..461
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 aacatacgaa cagatcactt aaagttcttc tgaagcaact taaagttatc aggcatgcat
       61 ggatcttgga ggaatcagat gtgcagtcag ggaccatagc acaagacagg cgtcttctac
      121 tggtgctacc agcaaatgct ggaagccggg aacactgggt acgttggaaa ccacgtgatg
      181 tgaagaagta agataaactg taggagaaaa gcatttcgta gtgggccatg aagcctttca
      241 ggacatgtat tgcagtatgg gccggcccat tacgcaattg gacgacaaca aagactagta
      301 ttagtaccac ctcggctatc cacatagatc aaagctgatt taaaagagtt gtgcagatga
      361 tccgtggcag gagaccgagg tctcggtttt agagctagaa atagcaagtt aaaataaggc
      421 tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg cttttttgtt ttagagctag
      481 aaatagcaag ttaaaataag gctagtccgt ttttagcgcg tgcatgcctg caggtccaca
      541 aattcgggtc aaggcggaag ccagcgcgcc accccacgtc agcaaatacg gaggcgcggg
      601 gttgacggcg tcacccggtc ctaacggcga ccaacaaacc agccagaaga aattacagta
      661 aaaaaaaagt aaattgcact ttgatccacc ttttattacc taagtctcaa tttggatcac
      721 ccttaaacct atcttttcaa tttgggccgg gttgtggttt ggactaccat gaacaacttt
      781 tcgtcatgtc taacttccct ttcagcaaac atatgaacca tatatagagg agatcggccg
      841 tatactagag ctgatgtgtt t
//
