LOCUS       sequence-107453-         560 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tetracycline biosensor. High copy plasmid that expresses GFP 
            proportional to tetracycline concentration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Rogers JK, Guzman CD, Taylor ND, Raman S, Anderson K, Church GM
  TITLE     Synthetic biosensors for precise gene control and real-time 
            monitoring of metabolites.
  JOURNAL   Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
  PUBMED    26152303
REFERENCE   2  (bases 1 to 560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Jul 7. pii: gkv616."
COMMENT     Sequence Label: pJKR-H-tetR
FEATURES             Location/Qualifiers
     source          1..560
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    185..203
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    210..228
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgcg aagcaacggc
       61 ccggagggtg gcgggcagga cgcccgccat aaactgccag gcatcaaatt aagcagaagg
      121 ccatccgtca ggatggcctt tttgcgtttc tacaaactct gctagcaagt aaggccgact
      181 cgagtcccta tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacat
      241 cagcaggacg catgaccgaa ttcattaaat ttaactttaa gaaggagata taatatgcgt
      301 aaaggtgaag aactgttcac cggtgttgtt ccgatcctgg ttgaactgga cggtgacgtt
      361 aacggtcaca aattctctgt tcgtggtgaa ggtgaaggtg acgctaccaa cggtaaactg
      421 accctgaaat tcatctgcac caccggtaaa ctgccggttc cgtggccgac cctggttacc
      481 accctgacct acggtgttca gtgcttcgct cgttacccgg accacatgaa acagcacgac
      541 ttcttcaaat ctgctatgcc
//
