LOCUS       sequence-107464-       12794 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  harbours marker genes HIS3, LEU2, URA3 and LYS2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12794)
  AUTHORS   Mulleder M, Campbell K, Matsarskaia O, Eckerstorfer F, Ralser M
  TITLE     Saccharomyces cerevisiae single-copy plasmids for auxotrophy 
            compensation, multiple marker selection, and for designing 
            metabolically cooperating communities.
  JOURNAL   F1000Res. 2016 Sep 20;5:2351. eCollection 2016.
  PUBMED    27830062
REFERENCE   2  (bases 1 to 12794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "F1000Res.";
            date: "2016-09-20"; volume: "5"; pages: "2351. eCollection 2016"
COMMENT     Sequence Label: pHLUK
FEATURES             Location/Qualifiers
     source          1..12794
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        315..502
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             503..1165
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        1404..1643
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
     CDS             1644..5822
                     /codon_start=1
                     /gene="S. cerevisiae LYS2"
                     /product="alpha-aminoadipate reductase, required for lysine
                     biosynthesis"
                     /label=LYS2
                     /note="yeast auxotrophic marker"
                     /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD
                     VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN
                     ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN
                     LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS
                     LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI
                     VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA
                     KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD
                     HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT
                     MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL
                     LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS
                     KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG
                     LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC
                     CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD
                     DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP
                     NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD
                     SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV
                     VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD
                     LLGRSPKNYSFKVFAHVRAKDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQF
                     GLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDFV
                     SSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMNSASGLTGGYGQSKWAAEYIIRRAGE
                     RGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVARV
                     VVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVID
                     RNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEVG
                     IYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA"
     CDS             6009..6812
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        7190..7597
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             7598..8692
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     rep_origin      complement(9255..9710)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9392..9413)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9604..9623
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9836..9858
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9850..9867
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9851..9867
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9874..9893
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9874..9892
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    9901..10008
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(10020..10038)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10059..10075)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10059..10075)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10072..10094)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10083..10099
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10107..10113,10114..10131,10132..10137))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10152..10173
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10290..10307)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10461..11049)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10541..10560)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11220..12011,12012..12080))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11843..11862
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12081..12185)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     12767..12785
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcggacg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataattc cgttttaaga gcttggtgag cgctaggagt cactgccagg tatcgtttga
      241 acacggcatt agtcagggaa gtcataacac agtcctttcc cgcaattttc tttttctatt
      301 actcttggcc tcctctagta cactctatat ttttttatgc ctcggtaatg attttcattt
      361 ttttttttcc acctagcgga tgactctttt tttttcttag cgattggcat tatcacataa
      421 tgaattatac attatataaa gtaatgtgat ttcttcgaag aatatactaa aaaatgagca
      481 ggcaagataa acgaaggcaa agatgacaga gcagaaagcc ctagtaaagc gtattacaaa
      541 tgaaaccaag attcagattg cgatctcttt aaagggtggt cccctagcga tagagcactc
      601 gatcttccca gaaaaagagg cagaagcagt agcagaacag gccacacaat cgcaagtgat
      661 taacgtccac acaggtatag ggtttctgga ccatatgata catgctctgg ccaagcattc
      721 cggctggtcg ctaatcgttg agtgcattgg tgacttacac atagacgacc atcacaccac
      781 tgaagactgc gggattgctc tcggtcaagc ttttaaagag gccctagggg ccgtgcgtgg
      841 agtaaaaagg tttggatcag gatttgcgcc tttggatgag gcactttcca gagcggtggt
      901 agatctttcg aacaggccgt acgcagttgt cgaacttggt ttgcaaaggg agaaagtagg
      961 agatctctct tgcgagatga tcccgcattt tcttgaaagc tttgcagagg ctagcagaat
     1021 taccctccac gttgattgtc tgcgaggcaa gaatgatcat caccgtagtg agagtgcgtt
     1081 caaggctctt gcggttgcca taagagaagc cacctcgccc aatggtacca acgatgttcc
     1141 ctccaccaaa ggtgttctta tgtagtgaca ccgattattt aaagctgcag catacgatat
     1201 atatacatgt gtatatatgt atacctatga atgtcagtaa gtatgtatac gaacagtatg
     1261 atactgaaga tgacaaggta atgcatcatt ctatacgtgt cattctgaac gaggcgcgct
     1321 ttcctttttt ctttttgctt tttctttttt tttctcttga actcgacgcg tcgaggaaaa
     1381 ctcttcaata gttttgccag cggaattcca cttgcaatta cataaaaaat tccggcggtt
     1441 tttcgcgtgt gactcaatgt cgaaatacct gcctaatgaa catgaacatc gcccaaatgt
     1501 atttgaagac ccgctgggag aagttcaaga tatataagta acaagcagcc aatagtataa
     1561 aaaaaaatct gagtttatta cctttcctgg aatttcagtg aaaaactgct aattatagag
     1621 agatatcaca gagttactca ctaatgacta acgaaaaggt ctggatagag aagttggata
     1681 atccaactct ttcagtgtta ccacatgact ttttacgccc acaacaagaa ccttatacga
     1741 aacaagctac atattcgtta cagctacctc agctcgatgt gcctcatgat agtttttcta
     1801 acaaatacgc tgtcgctttg agtgtatggg ctgcattgat atatagagta accggtgacg
     1861 atgatattgt tctttatatt gcgaataaca aaatcttaag attcaatatt caaccaacgt
     1921 ggtcatttaa tgagctgtat tctacaatta acaatgagtt gaacaagctc aattctattg
     1981 aggccaattt ttcctttgac gagctagctg aaaaaattca aagttgccaa gatctggaaa
     2041 ggacccctca gttgttccgt ttggcctttt tggaaaacca agatttcaaa ttagacgagt
     2101 tcaagcatca tttagtggac tttgctttga atttggatac cagtaataat gcgcatgttt
     2161 tgaacttaat ttataacagc ttactgtatt cgaatgaaag agtaaccatt gttgcggacc
     2221 aatttactca atatttgact gctgcgctaa gcgatccatc caattgcata actaaaatct
     2281 ctctgatcac cgcatcatcc aaggatagtt tacctgatcc aactaagaac ttgggctggt
     2341 gcgatttcgt ggggtgtatt cacgacattt tccaggacaa tgctgaagcc ttcccagaga
     2401 gaacctgtgt tgtggagact ccaacactaa attccgacaa gtcccgttct ttcacttatc
     2461 gcgacatcaa ccgcacttct aacatagttg cccattattt gattaaaaca ggtatcaaaa
     2521 gaggtgatgt agtgatgatc tattcttcta ggggtgtgga tttgatggta tgtgtgatgg
     2581 gtgtcttgaa agccggcgca accttttcag ttatcgaccc tgcatatccc ccagccagac
     2641 aaaccattta cttaggtgtt gctaaaccac gtgggttgat tgttattaga gctgctggac
     2701 aattggatca actagtagaa gattacatca atgatgaatt ggagattgtt tcaagaatca
     2761 attccatcgc tattcaagaa aatggtacca ttgaaggtgg caaattggac aatggcgagg
     2821 atgttttggc tccatatgat cactacaaag acaccagaac aggtgttgta gttggaccag
     2881 attccaaccc aaccctatct ttcacatctg gttccgaagg tattcctaag ggtgttcttg
     2941 gtagacattt ttccttggct tattatttca attggatgtc caaaaggttc aacttaacag
     3001 aaaatgataa attcacaatg ctgagcggta ttgcacatga tccaattcaa agagatatgt
     3061 ttacaccatt atttttaggt gcccaattgt atgtccctac tcaagatgat attggtacac
     3121 cgggccgttt agcggaatgg atgagtaagt atggttgcac agttacccat ttaacacctg
     3181 ccatgggtca attacttact gcccaagcta ctacaccatt ccctaagtta catcatgcgt
     3241 tctttgtggg tgacatttta acaaaacgtg attgtctgag gttacaaacc ttggcagaaa
     3301 attgccgtat tgttaatatg tacggtacca ctgaaacaca gcgtgcagtt tcttatttcg
     3361 aagttaaatc aaaaaatgac gatccaaact ttttgaaaaa attgaaagat gtcatgcctg
     3421 ctggtaaagg tatgttgaac gttcagctac tagttgttaa caggaacgat cgtactcaaa
     3481 tatgtggtat tggcgaaata ggtgagattt atgttcgtgc aggtggtttg gccgaaggtt
     3541 atagaggatt accagaattg aataaagaaa aatttgtgaa caactggttt gttgaaaaag
     3601 atcactggaa ttatttggat aaggataatg gtgaaccttg gagacaattc tggttaggtc
     3661 caagagatag attgtacaga acgggtgatt taggtcgtta tctaccaaac ggtgactgtg
     3721 aatgttgcgg tagggctgat gatcaagtta aaattcgtgg gttcagaatc gaattaggag
     3781 aaatagatac gcacatttcc caacatccat tggtaagaga aaacattact ttagttcgca
     3841 aaaatgccga caatgagcca acattgatca catttatggt cccaagattt gacaagccag
     3901 atgacttgtc taagttccaa agtgatgttc caaaggaggt tgaaactgac cctatagtta
     3961 agggcttaat cggttaccat cttttatcca aggacatcag gactttctta aagaaaagat
     4021 tggctagcta tgctatgcct tccttgattg tggttatgga taaactacca ttgaatccaa
     4081 atggtaaagt tgataagcct aaacttcaat tcccaactcc caagcaatta aatttggtag
     4141 ctgaaaatac agtttctgaa actgacgact ctcagtttac caatgttgag cgcgaggtta
     4201 gagacttatg gttaagtata ttacctacca agccagcatc tgtatcacca gatgattcgt
     4261 ttttcgattt aggtggtcat tctatcttgg ctaccaaaat gatttttacc ttaaagaaaa
     4321 agctgcaagt tgatttacca ttgggcacaa ttttcaagta tccaacgata aaggcctttg
     4381 ccgcggaaat tgacagaatt aaatcatcgg gtggatcatc tcaaggtgag gtcgtcgaaa
     4441 atgtcactgc aaattatgcg gaagacgcca agaaattggt tgagacgcta ccaagttcgt
     4501 acccctctcg agaatatttt gttgaaccta atagtgccga aggaaaaaca acaattaatg
     4561 tgtttgttac cggtgtcaca ggatttctgg gctcctacat ccttgcagat ttgttaggac
     4621 gttctccaaa gaactacagt ttcaaagtgt ttgcccacgt cagggccaag gatgaagaag
     4681 ctgcatttgc aagattacaa aaggcaggta tcacctatgg tacttggaac gaaaaatttg
     4741 cctcaaatat taaagttgta ttaggcgatt tatctaaaag ccaatttggt ctttcagatg
     4801 agaagtggat ggatttggca aacacagttg atataattat ccataatggt gcgttagttc
     4861 actgggttta tccatatgcc aaattgaggg atccaaatgt tatttcaact atcaatgtta
     4921 tgagcttagc cgccgtcggc aagccaaagt tctttgactt tgtttcctcc acttctactc
     4981 ttgacactga atactacttt aatttgtcag ataaacttgt tagcgaaggg aagccaggca
     5041 ttttagaatc agacgattta atgaactctg caagcgggct cactggtgga tatggtcagt
     5101 ccaaatgggc tgctgagtac atcattagac gtgcaggtga aaggggccta cgtgggtgta
     5161 ttgtcagacc aggttacgta acaggtgcct ctgccaatgg ttcttcaaac acagatgatt
     5221 tcttattgag atttttgaaa ggttcagtcc aattaggtaa gattccagat atcgaaaatt
     5281 ccgtgaatat ggttccagta gatcatgttg ctcgtgttgt tgttgctacg tctttgaatc
     5341 ctcccaaaga aaatgaattg gccgttgctc aagtaacggg tcacccaaga atattattca
     5401 aagactactt gtatacttta cacgattatg gttacgatgt cgaaatcgaa agctattcta
     5461 aatggaagaa atcattggag gcgtctgtta ttgacaggaa tgaagaaaat gcgttgtatc
     5521 ctttgctaca catggtctta gacaacttac ctgaaagtac caaagctccg gaactagacg
     5581 ataggaacgc cgtggcatct ttaaagaaag acaccgcatg gacaggtgtt gattggtcta
     5641 atggaatagg tgttactcca gaagaggttg gtatatatat tgcattttta aacaaggttg
     5701 gatttttacc tccaccaact cataatgaca aacttccact gccaagtata gaactaactc
     5761 aagcgcaaat aagtctagtt gcttcaggtg ctggtgctcg tggaagctcc gcagcagctt
     5821 aaggttgagc attacgtatg atatgtgcat gcgattcggt aatctccgaa cagaaggaag
     5881 aacgaaggaa ggagcacaga cttagattgg tatatatacg catatgtagt gttgaagaaa
     5941 catgaaattg cccagtattc ttaacccaac tgcacagaac aaaaacctgc aggaaacgaa
     6001 gataaatcat gtcgaaagct acatataagg aacgtgctgc tactcatcct agtcctgttg
     6061 ctgccaagct atttaatatc atgcacgaaa agcaaacaaa cttgtgtgct tcattggatg
     6121 ttcgtaccac caaggaatta ctggagttag ttgaagcatt aggtcccaaa atttgtttac
     6181 taaaaacaca tgtggatatc ttgactgatt tttccatgga gggcacagtt aagccgctaa
     6241 aggcattatc cgccaagtac aattttttac tcttcgaaga cagaaaattt gctgacattg
     6301 gtaatacagt caaattgcag tactctgcgg gtgtatacag aatagcagaa tgggcagaca
     6361 ttacgaatgc acacggtgtg gtgggcccag gtattgttag cggtttgaag caggcggcag
     6421 aagaagtaac aaaggaacct agaggccttt tgatgttagc agaattgtca tgcaagggct
     6481 ccctatctac tggagaatat actaagggta ctgttgacat tgcgaagagc gacaaagatt
     6541 ttgttatcgg ctttattgct caaagagaca tgggtggaag agatgaaggt tacgattggt
     6601 tgattatgac acccggtgtg ggtttagatg acaagggaga cgcattgggt caacagtata
     6661 gaaccgtgga tgatgtggtc tctacaggat ctgacattat tattgttgga agaggactat
     6721 ttgcaaaggg aagggatgct aaggtagagg gtgaacgtta cagaaaagca ggctgggaag
     6781 catatttgag aagatgcggc cagcaaaact aaaaaactgt attataagta aatgcatgta
     6841 tactaaactc acaaattaga gcttcaattt aattatatca gttattaccc tatgcggtgt
     6901 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgcgccat cgaggagaac
     6961 ttctagtata tctacatacc taatattatt gccttattaa aaatggaatc ccaacaatta
     7021 catcaaaatc cacattctct tcaaaatcaa ttgtcctgta cttccttgtt catgtgtgtt
     7081 caaaaacgtt atatttatag gataattata ctctatttct caacaagtaa ttggttgttt
     7141 ggccgagcgg tctaaggcgc ctgattcaag aaatatcttg accgcagtta actgtgggaa
     7201 tactcaggta tcgtaagatg caagagttcg aatctcttag caaccattat ttttttcctc
     7261 aacataacga gaacacacag gggcgctatc gcacagaatc aaattcgatg actggaaatt
     7321 ttttgttaat ttcagaggtc gcctgacgca tatacctttt tcaactgaaa aattgggaga
     7381 aaaaggaaag gtgagagcgc cggaaccggc ttttcatata gaatagagaa gcgttcatga
     7441 ctaaatgctt gcatcacaat acttgaagtt gacaatatta tttaaggacc tattgttttt
     7501 tccaataggt ggttagcaat cgtcttactt tctaactttt cttacctttt acatttcagc
     7561 aatatatata tatatatttc aaggatatac cattctaatg tctgccccta agaagatcgt
     7621 cgttttgcca ggtgaccacg ttggtcaaga aatcacagcc gaagccatta aggttcttaa
     7681 agctatttct gatgttcgtt ccaatgtcaa gttcgatttc gaaaatcatt taattggtgg
     7741 tgctgctatc gatgctacag gtgttccact tccagatgag gcgctggaag cctccaagaa
     7801 ggctgatgcc gttttgttag gtgctgtggg tggtcctaaa tggggtaccg gtagtgttag
     7861 acctgaacaa ggtttactaa aaatccgtaa agaacttcaa ttgtacgcca acttaagacc
     7921 atgtaacttt gcatccgact ctcttttaga cttatctcca atcaagccac aatttgctaa
     7981 aggtactgac ttcgttgttg tcagagaatt agtgggaggt atttactttg gtaagagaaa
     8041 ggaagacgat ggtgatggtg tcgcttggga tagtgaacaa tacaccgttc cagaagtgca
     8101 aagaatcaca agaatggccg ctttcatggc cctacaacat gagccaccat tgcctatttg
     8161 gtccttggat aaagctaatg ttttggcctc ttcaagatta tggagaaaaa ctgtggagga
     8221 aaccatcaag aacgaattcc ctacattgaa ggttcaacat caattgattg attctgccgc
     8281 catgatccta gttaagaacc caacccacct aaatggtatt ataatcacca gcaacatgtt
     8341 tggtgatatc atctccgatg aagcctccgt tatcccaggt tccttgggtt tgttgccatc
     8401 tgcgtccttg gcctctttgc cagacaagaa caccgcattt ggtttgtacg aaccatgcca
     8461 cggttctgct ccagatttgc caaagaataa ggtcaaccct atcgccacta tcttgtctgc
     8521 tgcaatgatg ttgaaattgt cattgaactt gcctgaagaa ggtaaggcca ttgaagatgc
     8581 agttaaaaag gttttggatg caggtatcag aactggtgat ttaggtggtt ccaacagtac
     8641 caccgaagtc ggtgatgctg tcgccgaaga agttaagaaa atccttgctt aaaaagattc
     8701 tcttttttta tgatatttgt acataaactt tataaatgaa attcataata gaaacgacac
     8761 gaaattacaa aatggaatat gttcataggg tagacgaaac tatatacgca atctacatac
     8821 atttatcaag aaggagaaaa aggaggatgt aaaggaatac aggtaagcaa attgatacta
     8881 atggctcaac gtgataagga aaaagaattg cactttaaca ttaatattga caaggaggag
     8941 ggcaccacac aaaaagttag gtgtaacaga aaatcatgaa actatgattc ctaatttata
     9001 tattggagga ttttctctaa aaaaaaaaaa atacaacaaa taaaaaacac tcaatgacct
     9061 gaccatttga tggagtttaa gtcaatacct tcttgaacca tttcccataa tggtgaaagt
     9121 tccctcaaga attttactct gtcagaaacg gccttacgac gtagtcgagg atctcctagg
     9181 ttaattaagt ttaaacagat cctatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     9241 aataccgcat caggaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
     9301 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     9361 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     9421 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     9481 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     9541 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     9601 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     9661 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca
     9721 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
     9781 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
     9841 tcacgacgtt gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattg
     9901 gagctccacc gcggtggcgg ccgctctaga actagtgggt cccccgggct gcaggaattc
     9961 gatatcaagc ttatcgatac cgtcgacctg gagggggggc ccggtaccag cttttgttcc
    10021 ctttagtgag ggttaatttc gagcttggcg taatcatggt catagctgtt tcctgtgtga
    10081 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
    10141 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
    10201 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
    10261 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
    10321 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
    10381 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
    10441 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
    10501 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
    10561 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
    10621 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
    10681 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
    10741 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
    10801 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
    10861 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
    10921 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
    10981 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
    11041 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
    11101 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
    11161 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
    11221 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
    11281 gttgcctgac tgcccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
    11341 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
    11401 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
    11461 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
    11521 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
    11581 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg aaaaaaagcg
    11641 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
    11701 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
    11761 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
    11821 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    11881 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    11941 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    12001 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    12061 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    12121 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    12181 ccgcgcacat ttccccgaaa agtgccacct atcgcttgcc tgtaacttac acgcgcctcg
    12241 tatcttttaa tgatggaata atttgggaat ttactctgtg tttatttatt tttatgtttt
    12301 gtatttggat tttagaaagt aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa
    12361 aataaacaaa ggtttaaaaa atttcaacaa aaagcgtact ttacatatat atttattaga
    12421 caagaaaagc agattaaata gatatacatt cgattaacga taagtaaaat gtaaaatcac
    12481 aggattttcg tgtgtggtct tctacacaga caagatgaaa caattcggca ttaatacctg
    12541 agagcaggaa gagcaagata aaaggtagta tttgttggcg atccccctag agtcttttac
    12601 atcttcggaa aacaaaaact attttttctt taatttcttt ttttactttc tatttttaat
    12661 ttatatattt atattaaaaa atttaaatta taattatttt tatagcacgt gatgaaaagg
    12721 acccccgtcc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
    12781 gaggcccttt cgtc
//
