LOCUS       sequence-107471-        9912 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  harbours marker genes HIS3, LEU2, URA3 and MET17.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9912)
  AUTHORS   Mulleder M, Campbell K, Matsarskaia O, Eckerstorfer F, Ralser M
  TITLE     Saccharomyces cerevisiae single-copy plasmids for auxotrophy 
            compensation, multiple marker selection, and for designing 
            metabolically cooperating communities.
  JOURNAL   F1000Res. 2016 Sep 20;5:2351. eCollection 2016.
  PUBMED    27830062
REFERENCE   2  (bases 1 to 9912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "F1000Res.";
            date: "2016-09-20"; volume: "5"; pages: "2351. eCollection 2016"
COMMENT     Sequence Label: pHLUM (version 2)
FEATURES             Location/Qualifiers
     source          1..9912
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     165..183
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(221..243)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     343..362
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        509..696
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             697..1359
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     CDS             complement(1613..2947)
                     /codon_start=1
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /product="O-acetylserine and O-acetylhomoserine 
                     sulfhydrylase, required for sulfur amino acid synthesis"
                     /label=MET17
                     /translation="MPSHFDTVQLHAGQENPGDNAHRSRAVPIYATTSYVFENSKHGSQ
                     LFGLEVPGYVYSRFQNPTSNVLEERIAALEGGAAALAVSSGQAAQTLAIQGLAHTGDNI
                     VSTSYLYGGTYNQFKISFKRFGIEARFVEGDNPEEFEKVFDERTKAVYLETIGNPKYNV
                     PDFEKIVAIAHKHGIPVVVDNTFGAGGYFCQPIKYGADIVTHSATKWIGGHGTTIGGII
                     VDSGKFPWKDYPEKFPQFSQPAEGYHGTIYNEAYGNLAYIVHVRTELLRDLGPLMNPFA
                     SFLLLQGVETLSLRAERHGENALKLAKWLEQSPYVSWVSYPGLASHSHHENAKKYLSNG
                     FGGVLSFGVKDLPNADKETDPFKLSGAQVVDNLKLASNLANVGDAKTLVIAPYFTTHKQ
                     LNDKEKLASGVTKDLIRVSVGIEFIDDIIADFQQSFETVFAGQKP"
     CDS             3344..4147
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        4525..4932
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             4933..6027
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     rep_origin      complement(6562..7021)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6699..6720)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6911..6930
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7147..7169
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7161..7178
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7162..7178
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7185..7204
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7185..7203
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    7212..7319
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(7331..7349)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7370..7386)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7370..7386)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7383..7405)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7394..7410
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7418..7424,7425..7442,7443..7448))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7463..7484
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7601..7618)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7772..8360)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7852..7871)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8531..9322,9323..9391))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9154..9173
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9392..9496)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    join(9526..9912,1..117)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
ORIGIN
        1 ttacatcttc ggaaaacaaa aactattttt tctttaattt ctttttttac tttctatttt
       61 taatttatat atttatatta aaaaatttaa attataatta tttttatagc acgtgatgaa
      121 aaggacccta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
      181 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
      241 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
      301 cgtcagcggg tgttggcgga cgtcggggct ggcttaacta tgcggcatca gagcagattg
      361 tactgagagt gcaccataaa ttcccgtttt aagagcttgg tgagcgctag gagtcactgc
      421 caggtatcgt ttgaacacgg cattagtcag ggaagtcata acacagtcct ttcccgcaat
      481 tttctttttc tattactctt ggcctcctct agtacactct atattttttt atgcctcggt
      541 aatgattttc attttttttt ttccacctag cggatgactc tttttttttc ttagcgattg
      601 gcattatcac ataatgaatt atacattata taaagtaatg tgatttcttc gaagaatata
      661 ctaaaaaatg agcaggcaag ataaacgaag gcaaagatga cagagcagaa agccctagta
      721 aagcgtatta caaatgaaac caagattcag attgcgatct ctttaaaggg tggtccccta
      781 gcgatagagc actcgatctt cccagaaaaa gaggcagaag cagtagcaga acaggccaca
      841 caatcgcaag tgattaacgt ccacacaggt atagggtttc tggaccatat gatacatgct
      901 ctggccaagc attccggctg gtcgctaatc gttgagtgca ttggtgactt acacatagac
      961 gaccatcaca ccactgaaga ctgcgggatt gctctcggtc aagcttttaa agaggcccta
     1021 ggggccgtgc gtggagtaaa aaggtttgga tcaggatttg cgcctttgga tgaggcactt
     1081 tccagagcgg tggtagatct ttcgaacagg ccgtacgcag ttgtcgaact tggtttgcaa
     1141 agggagaaag taggagatct ctcttgcgag atgatcccgc attttcttga aagctttgca
     1201 gaggctagca gaattaccct ccacgttgat tgtctgcgag gcaagaatga tcatcaccgt
     1261 agtgagagtg cgttcaaggc tcttgcggtt gccataagag aagccacctc gcccaatggt
     1321 accaacgatg ttccctccac caaaggtgtt cttatgtagt gacaccgatt atttaaagct
     1381 gcagcatacg atatatatac atgtgtatat atgtatacct atgaatgtca gtaagtatgt
     1441 atacgaacag tatgatactg aagatgacaa ggtaatgcat cattctatac gtgtcattct
     1501 gaacgaggcg cgctttcctt ttttcttttt gctttttctt tttttttctc ttgaactcga
     1561 gcttgtgaga gaaagtaggt ttatacataa ttttacaact cattacgcac actcatggtt
     1621 tttggccagc gaaaacagtt tcaaaagatt gctggaagtc tgcaataatg tcatcaataa
     1681 attcgatacc aacagagaca cgaattaagt ccttggtaac accagatgcc aacttttctt
     1741 tgtcatttaa ttgtttgtgg gtagtgaagt atggagcaat gactaaggtc ttggcatcac
     1801 caacattggc caagttagag gcaagcttta aattgtcaac aacttgagca ccagaaagtt
     1861 tgaatgggtc agtttccttg tcggcatttg gtaagtcttt tacaccgaaa gataagacac
     1921 caccgaaacc gttagataga tacttcttag cattttcatg atgagaatga gatgctaaac
     1981 cagggtatga aacccaagat acgtatgggg attgttctaa ccatttggct aacttcaatg
     2041 cattttcacc gtgtctttca gctctcaaag ataatgtttc aacaccttgt agtagcaaga
     2101 aagaggcaaa tgggttcatc aatggaccca aatctcttaa tagttcagtt ctaacatgaa
     2161 cgatgtatgc caagttaccg taggcttcat tgtagatagt accgtgatat ccttcggcag
     2221 gttgagagaa ttgagggaac ttttctgggt agtccttcca tgggaactta ccagagtcaa
     2281 caataatacc accgatagta gtaccatgac caccaatcca tttggtagca gaatgtgtta
     2341 caatatcagc accgtattta attggctgac agaagtaacc accggcacca aatgtgttgt
     2401 caacgacaac tggaataccg tgtttgtgag caattgcaac aattttttca aaatccggaa
     2461 cattgtactt tggattacca atggtttcca aataaacagc cttggttctt tcatcaaaga
     2521 ccttttcgaa ttcttctgga ttgtcacctt caacaaatct agcctcgata ccaaatcttt
     2581 tgaacgagat tttgaactgg ttataagtac caccgtataa gtaagaagtg gaaacgatgt
     2641 tgtcaccagt gtgtgccaaa ccttggatgg caagggtttg agcggcttga ccggaggaaa
     2701 cagccaaagc agcagcacca ccttctaaag cagcaattct ttcttccaaa acattactgg
     2761 ttgggttttg gaaacgggaa tagacgtaac ctggaacttc tagaccaaac aattgcgaac
     2821 catgcttaga gttttcgaaa acataagaag tggtggcgta aattggtaca gctctggatc
     2881 tgtgagcatt gtcaccaggg ttctcttggc cggcgtgtag ttgaacagta tcgaaatgag
     2941 atggcattgt atggatgggg gtaatagaat tgtatctatg tatctgacga ccctgtatta
     3001 cacgaaggaa atagtagatg aaaagacaag agagcaagaa aaaggaaaaa cttcctttct
     3061 aacagacgct ttacttaacc ttatatatat cttatttttt tcatcgagcg tgttcaattg
     3121 gacaaggtgc caccttttcg acacttcggt tattatgtta cacagttttc atgaggatgg
     3181 cgcatgcgat tcggtaatct ccgaacagaa ggaagaacga aggaaggagc acagacttag
     3241 attggtatat atacgcatat gtagtgttga agaaacatga aattgcccag tattcttaac
     3301 ccaactgcac agaacaaaaa cctgcaggaa acgaagataa atcatgtcga aagctacata
     3361 taaggaacgt gctgctactc atcctagtcc tgttgctgcc aagctattta atatcatgca
     3421 cgaaaagcaa acaaacttgt gtgcttcatt ggatgttcgt accaccaagg aattactgga
     3481 gttagttgaa gcattaggtc ccaaaatttg tttactaaaa acacatgtgg atatcttgac
     3541 tgatttttcc atggagggca cagttaagcc gctaaaggca ttatccgcca agtacaattt
     3601 tttactcttc gaagacagaa aatttgctga cattggtaat acagtcaaat tgcagtactc
     3661 tgcgggtgta tacagaatag cagaatgggc agacattacg aatgcacacg gtgtggtggg
     3721 cccaggtatt gttagcggtt tgaagcaggc ggcagaagaa gtaacaaagg aacctagagg
     3781 ccttttgatg ttagcagaat tgtcatgcaa gggctcccta tctactggag aatatactaa
     3841 gggtactgtt gacattgcga agagcgacaa agattttgtt atcggcttta ttgctcaaag
     3901 agacatgggt ggaagagatg aaggttacga ttggttgatt atgacacccg gtgtgggttt
     3961 agatgacaag ggagacgcat tgggtcaaca gtatagaacc gtggatgatg tggtctctac
     4021 aggatctgac attattattg ttggaagagg actatttgca aagggaaggg atgctaaggt
     4081 agagggtgaa cgttacagaa aagcaggctg ggaagcatat ttgagaagat gcggccagca
     4141 aaactaaaaa actgtattat aagtaaatgc atgtatacta aactcacaaa ttagagcttc
     4201 aatttaatta tatcagttat taccctatgc ggtgtgaaat accgcacaga tgcgtaagga
     4261 gaaaataccg catcaggcgc gccatcgagg agaacttcta gtatatctac atacctaata
     4321 ttattgcctt attaaaaatg gaatcccaac aattacatca aaatccacat tctcttcaaa
     4381 atcaattgtc ctgtacttcc ttgttcatgt gtgttcaaaa acgttatatt tataggataa
     4441 ttatactcta tttctcaaca agtaattggt tgtttggccg agcggtctaa ggcgcctgat
     4501 tcaagaaata tcttgaccgc agttaactgt gggaatactc aggtatcgta agatgcaaga
     4561 gttcgaatct cttagcaacc attatttttt tcctcaacat aacgagaaca cacaggggcg
     4621 ctatcgcaca gaatcaaatt cgatgactgg aaattttttg ttaatttcag aggtcgcctg
     4681 acgcatatac ctttttcaac tgaaaaattg ggagaaaaag gaaaggtgag agcgccggaa
     4741 ccggcttttc atatagaata gagaagcgtt catgactaaa tgcttgcatc acaatacttg
     4801 aagttgacaa tattatttaa ggacctattg ttttttccaa taggtggtta gcaatcgtct
     4861 tactttctaa cttttcttac cttttacatt tcagcaatat atatatatat atttcaagga
     4921 tataccattc taatgtctgc ccctaagaag atcgtcgttt tgccaggtga ccacgttggt
     4981 caagaaatca cagccgaagc cattaaggtt cttaaagcta tttctgatgt tcgttccaat
     5041 gtcaagttcg atttcgaaaa tcatttaatt ggtggtgctg ctatcgatgc tacaggtgtt
     5101 ccacttccag atgaggcgct ggaagcctcc aagaaggctg atgccgtttt gttaggtgct
     5161 gtgggtggtc ctaaatgggg taccggtagt gttagacctg aacaaggttt actaaaaatc
     5221 cgtaaagaac ttcaattgta cgccaactta agaccatgta actttgcatc cgactctctt
     5281 ttagacttat ctccaatcaa gccacaattt gctaaaggta ctgacttcgt tgttgtcaga
     5341 gaattagtgg gaggtattta ctttggtaag agaaaggaag acgatggtga tggtgtcgct
     5401 tgggatagtg aacaatacac cgttccagaa gtgcaaagaa tcacaagaat ggccgctttc
     5461 atggccctac aacatgagcc accattgcct atttggtcct tggataaagc taatgttttg
     5521 gcctcttcaa gattatggag aaaaactgtg gaggaaacca tcaagaacga attccctaca
     5581 ttgaaggttc aacatcaatt gattgattct gccgccatga tcctagttaa gaacccaacc
     5641 cacctaaatg gtattataat caccagcaac atgtttggtg atatcatctc cgatgaagcc
     5701 tccgttatcc caggttcctt gggtttgttg ccatctgcgt ccttggcctc tttgccagac
     5761 aagaacaccg catttggttt gtacgaacca tgccacggtt ctgctccaga tttgccaaag
     5821 aataaggtca accctatcgc cactatcttg tctgctgcaa tgatgttgaa attgtcattg
     5881 aacttgcctg aagaaggtaa ggccattgaa gatgcagtta aaaaggtttt ggatgcaggt
     5941 atcagaactg gtgatttagg tggttccaac agtaccaccg aagtcggtga tgctgtcgcc
     6001 gaagaagtta agaaaatcct tgcttaaaaa gattctcttt ttttatgata tttgtacata
     6061 aactttataa atgaaattca taatagaaac gacacgaaat tacaaaatgg aatatgttca
     6121 tagggtagac gaaactatat acgcaatcta catacattta tcaagaagga gaaaaaggag
     6181 gatgtaaagg aatacaggta agcaaattga tactaatggc tcaacgtgat aaggaaaaag
     6241 aattgcactt taacattaat attgacaagg aggagggcac cacacaaaaa gttaggtgta
     6301 acagaaaatc atgaaactat gattcctaat ttatatattg gaggattttc tctaaaaaaa
     6361 aaaaaataca acaaataaaa aacactcaat gacctgacca tttgatggag tttaagtcaa
     6421 taccttcttg aaccatttcc cataatggtg aaagttccct caagaatttt actctgtcag
     6481 aaacggcctt acgacgtagt cgaggatcct atgcggtgtg aaataccgca cagatgcgta
     6541 aggagaaaat accgcatcag gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     6601 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     6661 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     6721 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     6781 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     6841 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     6901 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggct ggcaagtgta
     6961 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     7021 tccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     7081 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     7141 ggttttccca gtcacgacgt tgtaaaacga cggccagtga attgtaatac gactcactat
     7201 agggcgaatt ggagctccac cgcggtggcg gccgctctag aactagtggg tcccccgggc
     7261 tgcaggaatt cgatatcaag cttatcgata ccgtcgacct ggaggggggg cccggtacca
     7321 gcttttgttc cctttagtga gggttaattt cgagcttggc gtaatcatgg tcatagctgt
     7381 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     7441 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     7501 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     7561 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     7621 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     7681 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     7741 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     7801 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     7861 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     7921 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     7981 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     8041 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     8101 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     8161 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     8221 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     8281 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     8341 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     8401 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     8461 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     8521 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     8581 gttcatccat agttgcctga ctgcccgtcg tgtagataac tacgatacgg gagggcttac
     8641 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     8701 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     8761 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     8821 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     8881 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     8941 gaaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     9001 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     9061 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     9121 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     9181 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     9241 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     9301 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     9361 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     9421 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     9481 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacggatcg cttgcctgta
     9541 acttacacgc gcctcgtatc ttttaatgat ggaataattt gggaatttac tctgtattta
     9601 tttattttta tgttttgtat ttggatttta gaaagtaaat aaagaaggta gaagagttac
     9661 ggaatgaaga aaaaaaaata aacaaaggtt taaaaaattt caacaaaaag cgtactttac
     9721 atatatattt attagacaag aaaagcagat taaatagata tacattcgat taacgataag
     9781 taaaatgtaa aatcacagga ttttcgtgtg tggtcttcta cacagacaag atgaaacaat
     9841 tcggcattaa tacctgagag caggaagagc aagataaaag gtagtatttg ttggcgatcc
     9901 ccctagagtc tt
//
