LOCUS       sequence-107476-        8815 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  harbours marker genes HIS3, LEU2 and MET17.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8815)
  AUTHORS   Mulleder M, Campbell K, Matsarskaia O, Eckerstorfer F, Ralser M
  TITLE     Saccharomyces cerevisiae single-copy plasmids for auxotrophy 
            compensation, multiple marker selection, and for designing 
            metabolically cooperating communities.
  JOURNAL   F1000Res. 2016 Sep 20;5:2351. eCollection 2016.
  PUBMED    27830062
REFERENCE   2  (bases 1 to 8815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "F1000Res.";
            date: "2016-09-20"; volume: "5"; pages: "2351. eCollection 2016"
COMMENT     Sequence Label: pHLM
FEATURES             Location/Qualifiers
     source          1..8815
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     165..183
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(221..243)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     343..362
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        507..694
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             695..1357
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     CDS             complement(1611..2945)
                     /codon_start=1
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /product="O-acetylserine and O-acetylhomoserine 
                     sulfhydrylase, required for sulfur amino acid synthesis"
                     /label=MET17
                     /translation="MPSHFDTVQLHAGQENPGDNAHRSRAVPIYATTSYVFENSKHGSQ
                     LFGLEVPGYVYSRFQNPTSNVLEERIAALEGGAAALAVSSGQAAQTLAIQGLAHTGDNI
                     VSTSYLYGGTYNQFKISFKRFGIEARFVEGDNPEEFEKVFDERTKAVYLETIGNPKYNV
                     PDFEKIVAIAHKHGIPVVVDNTFGAGGYFCQPIKYGADIVTHSATKWIGGHGTTIGGII
                     VDSGKFPWKDYPEKFPQFSQPAEGYHGTIYNEAYGNLAYIVHVRTELLRDLGPLMNPFA
                     SFLLLQGVETLSLRAERHGENALKLAKWLEQSPYVSWVSYPGLASHSHHENAKKYLSNG
                     FGGVLSFGVKDLPNADKETDPFKLSGAQVVDNLKLASNLANVGDAKTLVIAPYFTTHKQ
                     LNDKEKLASGVTKDLIRVSVGIEFIDDIIADFQQSFETVFAGQKP"
     promoter        3428..3835
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             3836..4930
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     rep_origin      complement(5465..5924)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5602..5623)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5814..5833
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6050..6072
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6064..6081
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6065..6081
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6088..6107
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6088..6106
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    6115..6222
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(6234..6252)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6273..6289)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6273..6289)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6286..6308)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6297..6313
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6321..6327,6328..6345,6346..6351))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6366..6387
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6504..6521)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6675..7263)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6755..6774)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7434..8225,8226..8294))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8057..8076
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8295..8399)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    join(8429..8815,1..117)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
ORIGIN
        1 ttacatcttc ggaaaacaaa aactattttt tctttaattt ctttttttac tttctatttt
       61 taatttatat atttatatta aaaaatttaa attataatta tttttatagc acgtgatgaa
      121 aaggacccta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
      181 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
      241 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
      301 cgtcagcggg tgttggcgga cgtcggggct ggcttaacta tgcggcatca gagcagattg
      361 tactgagagt gcaccataat tccgttttaa gagcttggtg agcgctagga gtcactgcca
      421 ggtatcgttt gaacacggca ttagtcaggg aagtcataac acagtccttt cccgcaattt
      481 tctttttcta ttactcttgg cctcctctag tacactctat atttttttat gcctcggtaa
      541 tgattttcat tttttttttt ccacctagcg gatgactctt tttttttctt agcgattggc
      601 attatcacat aatgaattat acattatata aagtaatgtg atttcttcga agaatatact
      661 aaaaaatgag caggcaagat aaacgaaggc aaagatgaca gagcagaaag ccctagtaaa
      721 gcgtattaca aatgaaacca agattcagat tgcgatctct ttaaagggtg gtcccctagc
      781 gatagagcac tcgatcttcc cagaaaaaga ggcagaagca gtagcagaac aggccacaca
      841 atcgcaagtg attaacgtcc acacaggtat agggtttctg gaccatatga tacatgctct
      901 ggccaagcat tccggctggt cgctaatcgt tgagtgcatt ggtgacttac acatagacga
      961 ccatcacacc actgaagact gcgggattgc tctcggtcaa gcttttaaag aggccctagg
     1021 ggccgtgcgt ggagtaaaaa ggtttggatc aggatttgcg cctttggatg aggcactttc
     1081 cagagcggtg gtagatcttt cgaacaggcc gtacgcagtt gtcgaacttg gtttgcaaag
     1141 ggagaaagta ggagatctct cttgcgagat gatcccgcat tttcttgaaa gctttgcaga
     1201 ggctagcaga attaccctcc acgttgattg tctgcgaggc aagaatgatc atcaccgtag
     1261 tgagagtgcg ttcaaggctc ttgcggttgc cataagagaa gccacctcgc ccaatggtac
     1321 caacgatgtt ccctccacca aaggtgttct tatgtagtga caccgattat ttaaagctgc
     1381 agcatacgat atatatacat gtgtatatat gtatacctat gaatgtcagt aagtatgtat
     1441 acgaacagta tgatactgaa gatgacaagg taatgcatca ttctatacgt gtcattctga
     1501 acgaggcgcg ctttcctttt ttctttttgc tttttctttt tttttctctt gaactcgagc
     1561 ttgtgagaga aagtaggttt atacataatt ttacaactca ttacgcacac tcatggtttt
     1621 tggccagcga aaacagtttc aaaagattgc tggaagtctg caataatgtc atcaataaat
     1681 tcgataccaa cagagacacg aattaagtcc ttggtaacac cagatgccaa cttttctttg
     1741 tcatttaatt gtttgtgggt agtgaagtat ggagcaatga ctaaggtctt ggcatcacca
     1801 acattggcca agttagaggc aagctttaaa ttgtcaacaa cttgagcacc agaaagtttg
     1861 aatgggtcag tttccttgtc ggcatttggt aagtctttta caccgaaaga taagacacca
     1921 ccgaaaccgt tagatagata cttcttagca ttttcatgat gagaatgaga tgctaaacca
     1981 gggtatgaaa cccaagatac gtatggggat tgttctaacc atttggctaa cttcaatgca
     2041 ttttcaccgt gtctttcagc tctcaaagat aatgtttcaa caccttgtag tagcaagaaa
     2101 gaggcaaatg ggttcatcaa tggacccaaa tctcttaata gttcagttct aacatgaacg
     2161 atgtatgcca agttaccgta ggcttcattg tagatagtac cgtgatatcc ttcggcaggt
     2221 tgagagaatt gagggaactt ttctgggtag tccttccatg ggaacttacc agagtcaaca
     2281 ataataccac cgatagtagt accatgacca ccaatccatt tggtagcaga atgtgttaca
     2341 atatcagcac cgtatttaat tggctgacag aagtaaccac cggcaccaaa tgtgttgtca
     2401 acgacaactg gaataccgtg tttgtgagca attgcaacaa ttttttcaaa atccggaaca
     2461 ttgtactttg gattaccaat ggtttccaaa taaacagcct tggttctttc atcaaagacc
     2521 ttttcgaatt cttctggatt gtcaccttca acaaatctag cctcgatacc aaatcttttg
     2581 aacgagattt tgaactggtt ataagtacca ccgtataagt aagaagtgga aacgatgttg
     2641 tcaccagtgt gtgccaaacc ttggatggca agggtttgag cggcttgacc ggaggaaaca
     2701 gccaaagcag cagcaccacc ttctaaagca gcaattcttt cttccaaaac attactggtt
     2761 gggttttgga aacgggaata gacgtaacct ggaacttcta gaccaaacaa ttgcgaacca
     2821 tgcttagagt tttcgaaaac ataagaagtg gtggcgtaaa ttggtacagc tctggatctg
     2881 tgagcattgt caccagggtt ctcttggccg gcgtgtagtt gaacagtatc gaaatgagat
     2941 ggcattgtat ggatgggggt aatagaattg tatctatgta tctgacgacc ctgtattaca
     3001 cgaaggaaat agtagatgaa aagacaagag agcaagaaaa aggaaaaact tcctttctaa
     3061 cagacgcttt acttaacctt atatatatct tatttttttc atcgagcgtg ttcaattgga
     3121 caaggtgcca ccttttcgac acttcggtta ttatgttaca cagttttcat gaggatggcg
     3181 cgcgccatcg aggagaactt ctagtatatc tacataccta atattattgc cttattaaaa
     3241 atggaatccc aacaattaca tcaaaatcca cattctcttc aaaatcaatt gtcctgtact
     3301 tccttgttca tgtgtgttca aaaacgttat atttatagga taattatact ctatttctca
     3361 acaagtaatt ggttgtttgg ccgagcggtc taaggcgcct gattcaagaa atatcttgac
     3421 cgcagttaac tgtgggaata ctcaggtatc gtaagatgca agagttcgaa tctcttagca
     3481 accattattt ttttcctcaa cataacgaga acacacaggg gcgctatcgc acagaatcaa
     3541 attcgatgac tggaaatttt ttgttaattt cagaggtcgc ctgacgcata tacctttttc
     3601 aactgaaaaa ttgggagaaa aaggaaaggt gagagcgccg gaaccggctt ttcatataga
     3661 atagagaagc gttcatgact aaatgcttgc atcacaatac ttgaagttga caatattatt
     3721 taaggaccta ttgttttttc caataggtgg ttagcaatcg tcttactttc taacttttct
     3781 taccttttac atttcagcaa tatatatata tatatttcaa ggatatacca ttctaatgtc
     3841 tgcccctaag aagatcgtcg ttttgccagg tgaccacgtt ggtcaagaaa tcacagccga
     3901 agccattaag gttcttaaag ctatttctga tgttcgttcc aatgtcaagt tcgatttcga
     3961 aaatcattta attggtggtg ctgctatcga tgctacaggt gttccacttc cagatgaggc
     4021 gctggaagcc tccaagaagg ctgatgccgt tttgttaggt gctgtgggtg gtcctaaatg
     4081 gggtaccggt agtgttagac ctgaacaagg tttactaaaa atccgtaaag aacttcaatt
     4141 gtacgccaac ttaagaccat gtaactttgc atccgactct cttttagact tatctccaat
     4201 caagccacaa tttgctaaag gtactgactt cgttgttgtc agagaattag tgggaggtat
     4261 ttactttggt aagagaaagg aagacgatgg tgatggtgtc gcttgggata gtgaacaata
     4321 caccgttcca gaagtgcaaa gaatcacaag aatggccgct ttcatggccc tacaacatga
     4381 gccaccattg cctatttggt ccttggataa agctaatgtt ttggcctctt caagattatg
     4441 gagaaaaact gtggaggaaa ccatcaagaa cgaattccct acattgaagg ttcaacatca
     4501 attgattgat tctgccgcca tgatcctagt taagaaccca acccacctaa atggtattat
     4561 aatcaccagc aacatgtttg gtgatatcat ctccgatgaa gcctccgtta tcccaggttc
     4621 cttgggtttg ttgccatctg cgtccttggc ctctttgcca gacaagaaca ccgcatttgg
     4681 tttgtacgaa ccatgccacg gttctgctcc agatttgcca aagaataagg tcaaccctat
     4741 cgccactatc ttgtctgctg caatgatgtt gaaattgtca ttgaacttgc ctgaagaagg
     4801 taaggccatt gaagatgcag ttaaaaaggt tttggatgca ggtatcagaa ctggtgattt
     4861 aggtggttcc aacagtacca ccgaagtcgg tgatgctgtc gccgaagaag ttaagaaaat
     4921 ccttgcttaa aaagattctc tttttttatg atatttgtac ataaacttta taaatgaaat
     4981 tcataataga aacgacacga aattacaaaa tggaatatgt tcatagggta gacgaaacta
     5041 tatacgcaat ctacatacat ttatcaagaa ggagaaaaag gaggatgtaa aggaatacag
     5101 gtaagcaaat tgatactaat ggctcaacgt gataaggaaa aagaattgca ctttaacatt
     5161 aatattgaca aggaggaggg caccacacaa aaagttaggt gtaacagaaa atcatgaaac
     5221 tatgattcct aatttatata ttggaggatt ttctctaaaa aaaaaaaaat acaacaaata
     5281 aaaaacactc aatgacctga ccatttgatg gagtttaagt caataccttc ttgaaccatt
     5341 tcccataatg gtgaaagttc cctcaagaat tttactctgt cagaaacggc cttacgacgt
     5401 agtcgaggat cctatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     5461 caggaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     5521 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     5581 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     5641 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     5701 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     5761 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     5821 aaagcgaaag gagcgggcgc tagggcgctg gctggcaagt gtagcggtca cgctgcgcgt
     5881 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtccattc gccattcagg
     5941 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     6001 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     6061 cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga attggagctc
     6121 caccgcggtg gcggccgctc tagaactagt gggtcccccg ggctgcagga attcgatatc
     6181 aagcttatcg ataccgtcga cctggagggg gggcccggta ccagcttttg ttccctttag
     6241 tgagggttaa tttcgagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     6301 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     6361 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     6421 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     6481 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     6541 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     6601 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     6661 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     6721 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     6781 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     6841 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     6901 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     6961 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     7021 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     7081 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     7141 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     7201 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     7261 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     7321 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     7381 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     7441 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     7501 tgactgcccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     7561 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     7621 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     7681 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     7741 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     7801 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgaaaaaa agcggttagc
     7861 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     7921 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     7981 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     8041 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     8101 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     8161 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     8221 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     8281 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     8341 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     8401 acatttcccc gaaaagtgcc acctgacgga tcgcttgcct gtaacttaca cgcgcctcgt
     8461 atcttttaat gatggaataa tttgggaatt tactctgtat ttatttattt ttatgttttg
     8521 tatttggatt ttagaaagta aataaagaag gtagaagagt tacggaatga agaaaaaaaa
     8581 ataaacaaag gtttaaaaaa tttcaacaaa aagcgtactt tacatatata tttattagac
     8641 aagaaaagca gattaaatag atatacattc gattaacgat aagtaaaatg taaaatcaca
     8701 ggattttcgt gtgtggtctt ctacacagac aagatgaaac aattcggcat taatacctga
     8761 gagcaggaag agcaagataa aaggtagtat ttgttggcga tccccctaga gtctt
//
