LOCUS       sequence-107478-        8675 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  harbours marker genes LEU2, URA3 and MET17.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8675)
  AUTHORS   Mulleder M, Campbell K, Matsarskaia O, Eckerstorfer F, Ralser M
  TITLE     Saccharomyces cerevisiae single-copy plasmids for auxotrophy 
            compensation, multiple marker selection, and for designing 
            metabolically cooperating communities.
  JOURNAL   F1000Res. 2016 Sep 20;5:2351. eCollection 2016.
  PUBMED    27830062
REFERENCE   2  (bases 1 to 8675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "F1000Res.";
            date: "2016-09-20"; volume: "5"; pages: "2351. eCollection 2016"
COMMENT     Sequence Label: pLUM
FEATURES             Location/Qualifiers
     source          1..8675
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     165..183
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(221..243)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(376..1710)
                     /codon_start=1
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /product="O-acetylserine and O-acetylhomoserine 
                     sulfhydrylase, required for sulfur amino acid synthesis"
                     /label=MET17
                     /translation="MPSHFDTVQLHAGQENPGDNAHRSRAVPIYATTSYVFENSKHGSQ
                     LFGLEVPGYVYSRFQNPTSNVLEERIAALEGGAAALAVSSGQAAQTLAIQGLAHTGDNI
                     VSTSYLYGGTYNQFKISFKRFGIEARFVEGDNPEEFEKVFDERTKAVYLETIGNPKYNV
                     PDFEKIVAIAHKHGIPVVVDNTFGAGGYFCQPIKYGADIVTHSATKWIGGHGTTIGGII
                     VDSGKFPWKDYPEKFPQFSQPAEGYHGTIYNEAYGNLAYIVHVRTELLRDLGPLMNPFA
                     SFLLLQGVETLSLRAERHGENALKLAKWLEQSPYVSWVSYPGLASHSHHENAKKYLSNG
                     FGGVLSFGVKDLPNADKETDPFKLSGAQVVDNLKLASNLANVGDAKTLVIAPYFTTHKQ
                     LNDKEKLASGVTKDLIRVSVGIEFIDDIIADFQQSFETVFAGQKP"
     CDS             2107..2910
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        3288..3695
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             3696..4790
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     rep_origin      complement(5325..5784)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5462..5483)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5674..5693
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5910..5932
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5924..5941
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5925..5941
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5948..5967
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5948..5966
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    5975..6082
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(6094..6112)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6133..6149)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6133..6149)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6146..6168)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6157..6173
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6181..6187,6188..6205,6206..6211))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6226..6247
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6364..6381)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6535..7123)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6615..6634)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7294..8085,8086..8154))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7917..7936
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8155..8259)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    join(8289..8675,1..117)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
ORIGIN
        1 ttacatcttc ggaaaacaaa aactattttt tctttaattt ctttttttac tttctatttt
       61 taatttatat atttatatta aaaaatttaa attataatta tttttatagc acgtgatgaa
      121 aaggacccta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
      181 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
      241 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
      301 cgtcagcggg tgttggcggt cgagcttgtg agagaaagta ggtttataca taattttaca
      361 actcattacg cacactcatg gtttttggcc agcgaaaaca gtttcaaaag attgctggaa
      421 gtctgcaata atgtcatcaa taaattcgat accaacagag acacgaatta agtccttggt
      481 aacaccagat gccaactttt ctttgtcatt taattgtttg tgggtagtga agtatggagc
      541 aatgactaag gtcttggcat caccaacatt ggccaagtta gaggcaagct ttaaattgtc
      601 aacaacttga gcaccagaaa gtttgaatgg gtcagtttcc ttgtcggcat ttggtaagtc
      661 ttttacaccg aaagataaga caccaccgaa accgttagat agatacttct tagcattttc
      721 atgatgagaa tgagatgcta aaccagggta tgaaacccaa gatacgtatg gggattgttc
      781 taaccatttg gctaacttca atgcattttc accgtgtctt tcagctctca aagataatgt
      841 ttcaacacct tgtagtagca agaaagaggc aaatgggttc atcaatggac ccaaatctct
      901 taatagttca gttctaacat gaacgatgta tgccaagtta ccgtaggctt cattgtagat
      961 agtaccgtga tatccttcgg caggttgaga gaattgaggg aacttttctg ggtagtcctt
     1021 ccatgggaac ttaccagagt caacaataat accaccgata gtagtaccat gaccaccaat
     1081 ccatttggta gcagaatgtg ttacaatatc agcaccgtat ttaattggct gacagaagta
     1141 accaccggca ccaaatgtgt tgtcaacgac aactggaata ccgtgtttgt gagcaattgc
     1201 aacaattttt tcaaaatccg gaacattgta ctttggatta ccaatggttt ccaaataaac
     1261 agccttggtt ctttcatcaa agaccttttc gaattcttct ggattgtcac cttcaacaaa
     1321 tctagcctcg ataccaaatc ttttgaacga gattttgaac tggttataag taccaccgta
     1381 taagtaagaa gtggaaacga tgttgtcacc agtgtgtgcc aaaccttgga tggcaagggt
     1441 ttgagcggct tgaccggagg aaacagccaa agcagcagca ccaccttcta aagcagcaat
     1501 tctttcttcc aaaacattac tggttgggtt ttggaaacgg gaatagacgt aacctggaac
     1561 ttctagacca aacaattgcg aaccatgctt agagttttcg aaaacataag aagtggtggc
     1621 gtaaattggt acagctctgg atctgtgagc attgtcacca gggttctctt ggccggcgtg
     1681 tagttgaaca gtatcgaaat gagatggcat tgtatggatg ggggtaatag aattgtatct
     1741 atgtatctga cgaccctgta ttacacgaag gaaatagtag atgaaaagac aagagagcaa
     1801 gaaaaaggaa aaacttcctt tctaacagac gctttactta accttatata tatcttattt
     1861 ttttcatcga gcgtgttcaa ttggacaagg tgccaccttt tcgacacttc ggttattatg
     1921 ttacacagtt ttcatgagga tggcgcatgc gattcggtaa tctccgaaca gaaggaagaa
     1981 cgaaggaagg agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca
     2041 tgaaattgcc cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga
     2101 taaatcatgt cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct
     2161 gccaagctat ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt
     2221 cgtaccacca aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta
     2281 aaaacacatg tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag
     2341 gcattatccg ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt
     2401 aatacagtca aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt
     2461 acgaatgcac acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa
     2521 gaagtaacaa aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc
     2581 ctatctactg gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt
     2641 gttatcggct ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg
     2701 attatgacac ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga
     2761 accgtggatg atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt
     2821 gcaaagggaa gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca
     2881 tatttgagaa gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata
     2941 ctaaactcac aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga
     3001 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgcgccatcg aggagaactt
     3061 ctagtatatc tacataccta atattattgc cttattaaaa atggaatccc aacaattaca
     3121 tcaaaatcca cattctcttc aaaatcaatt gtcctgtact tccttgttca tgtgtgttca
     3181 aaaacgttat atttatagga taattatact ctatttctca acaagtaatt ggttgtttgg
     3241 ccgagcggtc taaggcgcct gattcaagaa atatcttgac cgcagttaac tgtgggaata
     3301 ctcaggtatc gtaagatgca agagttcgaa tctcttagca accattattt ttttcctcaa
     3361 cataacgaga acacacaggg gcgctatcgc acagaatcaa attcgatgac tggaaatttt
     3421 ttgttaattt cagaggtcgc ctgacgcata tacctttttc aactgaaaaa ttgggagaaa
     3481 aaggaaaggt gagagcgccg gaaccggctt ttcatataga atagagaagc gttcatgact
     3541 aaatgcttgc atcacaatac ttgaagttga caatattatt taaggaccta ttgttttttc
     3601 caataggtgg ttagcaatcg tcttactttc taacttttct taccttttac atttcagcaa
     3661 tatatatata tatatttcaa ggatatacca ttctaatgtc tgcccctaag aagatcgtcg
     3721 ttttgccagg tgaccacgtt ggtcaagaaa tcacagccga agccattaag gttcttaaag
     3781 ctatttctga tgttcgttcc aatgtcaagt tcgatttcga aaatcattta attggtggtg
     3841 ctgctatcga tgctacaggt gttccacttc cagatgaggc gctggaagcc tccaagaagg
     3901 ctgatgccgt tttgttaggt gctgtgggtg gtcctaaatg gggtaccggt agtgttagac
     3961 ctgaacaagg tttactaaaa atccgtaaag aacttcaatt gtacgccaac ttaagaccat
     4021 gtaactttgc atccgactct cttttagact tatctccaat caagccacaa tttgctaaag
     4081 gtactgactt cgttgttgtc agagaattag tgggaggtat ttactttggt aagagaaagg
     4141 aagacgatgg tgatggtgtc gcttgggata gtgaacaata caccgttcca gaagtgcaaa
     4201 gaatcacaag aatggccgct ttcatggccc tacaacatga gccaccattg cctatttggt
     4261 ccttggataa agctaatgtt ttggcctctt caagattatg gagaaaaact gtggaggaaa
     4321 ccatcaagaa cgaattccct acattgaagg ttcaacatca attgattgat tctgccgcca
     4381 tgatcctagt taagaaccca acccacctaa atggtattat aatcaccagc aacatgtttg
     4441 gtgatatcat ctccgatgaa gcctccgtta tcccaggttc cttgggtttg ttgccatctg
     4501 cgtccttggc ctctttgcca gacaagaaca ccgcatttgg tttgtacgaa ccatgccacg
     4561 gttctgctcc agatttgcca aagaataagg tcaaccctat cgccactatc ttgtctgctg
     4621 caatgatgtt gaaattgtca ttgaacttgc ctgaagaagg taaggccatt gaagatgcag
     4681 ttaaaaaggt tttggatgca ggtatcagaa ctggtgattt aggtggttcc aacagtacca
     4741 ccgaagtcgg tgatgctgtc gccgaagaag ttaagaaaat ccttgcttaa aaagattctc
     4801 tttttttatg atatttgtac ataaacttta taaatgaaat tcataataga aacgacacga
     4861 aattacaaaa tggaatatgt tcatagggta gacgaaacta tatacgcaat ctacatacat
     4921 ttatcaagaa ggagaaaaag gaggatgtaa aggaatacag gtaagcaaat tgatactaat
     4981 ggctcaacgt gataaggaaa aagaattgca ctttaacatt aatattgaca aggaggaggg
     5041 caccacacaa aaagttaggt gtaacagaaa atcatgaaac tatgattcct aatttatata
     5101 ttggaggatt ttctctaaaa aaaaaaaaat acaacaaata aaaaacactc aatgacctga
     5161 ccatttgatg gagtttaagt caataccttc ttgaaccatt tcccataatg gtgaaagttc
     5221 cctcaagaat tttactctgt cagaaacggc cttacgacgt agtcgaggat cctatgcggt
     5281 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggaaattg taagcgttaa
     5341 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     5401 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     5461 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     5521 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     5581 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     5641 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     5701 tagggcgctg gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     5761 ttaatgcgcc gctacagggc gcgtccattc gccattcagg ctgcgcaact gttgggaagg
     5821 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
     5881 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
     5941 tgaattgtaa tacgactcac tatagggcga attggagctc caccgcggtg gcggccgctc
     6001 tagaactagt gggtcccccg ggctgcagga attcgatatc aagcttatcg ataccgtcga
     6061 cctggagggg gggcccggta ccagcttttg ttccctttag tgagggttaa tttcgagctt
     6121 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     6181 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     6241 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     6301 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     6361 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     6421 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     6481 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     6541 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     6601 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     6661 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     6721 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     6781 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     6841 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     6901 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     6961 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     7021 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     7081 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     7141 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     7201 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     7261 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     7321 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactgcccg tcgtgtagat
     7381 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     7441 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     7501 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     7561 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     7621 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     7681 agttacatga tcccccatgt tgtgaaaaaa agcggttagc tccttcggtc ctccgatcgt
     7741 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     7801 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     7861 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     7921 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     7981 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     8041 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     8101 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     8161 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     8221 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     8281 acctgacgga tcgcttgcct gtaacttaca cgcgcctcgt atcttttaat gatggaataa
     8341 tttgggaatt tactctgtat ttatttattt ttatgttttg tatttggatt ttagaaagta
     8401 aataaagaag gtagaagagt tacggaatga agaaaaaaaa ataaacaaag gtttaaaaaa
     8461 tttcaacaaa aagcgtactt tacatatata tttattagac aagaaaagca gattaaatag
     8521 atatacattc gattaacgat aagtaaaatg taaaatcaca ggattttcgt gtgtggtctt
     8581 ctacacagac aagatgaaac aattcggcat taatacctga gagcaggaag agcaagataa
     8641 aaggtagtat ttgttggcga tccccctaga gtctt
//
