LOCUS       sequence-107479-         554 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA + 2x MS2(wt+f6) with MCP-VP64 effector for mammalian cells, 
            marked by BFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Zalatan JG, Lee ME, Almeida R, Gilbert LA, Whitehead EH, La Russa M,
            Tsai JC, Weissman JS, Dueber JE, Qi LS, Lim WA
  TITLE     Engineering Complex Synthetic Transcriptional Programs with CRISPR 
            RNA Scaffolds.
  JOURNAL   Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052.
  PUBMED    25533786
REFERENCE   2  (bases 1 to 554)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052."
COMMENT     Sequence Label: pJZC116
FEATURES             Location/Qualifiers
     source          1..554
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    60..93
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gattatcgat aagcttgata tcgaattgta cctagtggaa ccggaaccct taaacatgta
       61 taacttcgta taatgtatgc tatacgaagt tattaggtcc ctcgacgaat tcttaattaa
      121 gcttgtgccc cagtttgcta gggaggtcgc agtatctggc cactgccacc tcgtgctgct
      181 cgacgtaggt ctcgttgttg gcctccttga ttctttccag tctgtagtcc acatagtaga
      241 cgccaggcat cttgaggttc ttagcgggtt tcttggatct atatgtggtc ttgatgtttg
      301 cgatcagatg gctcccgccc acgagcttca gggccatgtc gtttctgcct tccaggccgc
      361 cgtcagcggg gtacagcgtc tcggtgaagg cctcccagcc gagtgttttc ttctgcatca
      421 cagggccgtt ggatgtgaag ttcacccctc tgatcttgac gttgtagatg aggcagccgt
      481 cctggaggct ggtgtcctgg gtagcggtca gcacgccccc gtcttcgtat gtggtgactc
      541 tctcccatgt gaag
//
