LOCUS       sequence-107498-       11699 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  harbours marker genes HIS3, LEU2 and LYS2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11699)
  AUTHORS   Mulleder M, Campbell K, Matsarskaia O, Eckerstorfer F, Ralser M
  TITLE     Saccharomyces cerevisiae single-copy plasmids for auxotrophy 
            compensation, multiple marker selection, and for designing 
            metabolically cooperating communities.
  JOURNAL   F1000Res. 2016 Sep 20;5:2351. eCollection 2016.
  PUBMED    27830062
REFERENCE   2  (bases 1 to 11699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "F1000Res.";
            date: "2016-09-20"; volume: "5"; pages: "2351. eCollection 2016"
COMMENT     Sequence Label: pHLK
FEATURES             Location/Qualifiers
     source          1..11699
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        315..502
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             503..1165
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        1404..1643
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
     CDS             1644..5822
                     /codon_start=1
                     /gene="S. cerevisiae LYS2"
                     /product="alpha-aminoadipate reductase, required for lysine
                     biosynthesis"
                     /label=LYS2
                     /note="yeast auxotrophic marker"
                     /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD
                     VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN
                     ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN
                     LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS
                     LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI
                     VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA
                     KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD
                     HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT
                     MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL
                     LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS
                     KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG
                     LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC
                     CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD
                     DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP
                     NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD
                     SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV
                     VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD
                     LLGRSPKNYSFKVFAHVRAKDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQF
                     GLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDFV
                     SSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMNSASGLTGGYGQSKWAAEYIIRRAGE
                     RGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVARV
                     VVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVID
                     RNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEVG
                     IYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA"
     promoter        6095..6502
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             6503..7597
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     rep_origin      complement(8160..8615)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8297..8318)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8509..8528
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8741..8763
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8755..8772
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8756..8772
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8779..8798
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8779..8797
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    8806..8913
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(8925..8943)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8964..8980)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8964..8980)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8977..8999)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8988..9004
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9012..9018,9019..9036,9037..9042))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9057..9078
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9195..9212)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9366..9954)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9446..9465)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10125..10916,10917..10985))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10748..10767
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10986..11090)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11672..11690
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcggacg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataattc cgttttaaga gcttggtgag cgctaggagt cactgccagg tatcgtttga
      241 acacggcatt agtcagggaa gtcataacac agtcctttcc cgcaattttc tttttctatt
      301 actcttggcc tcctctagta cactctatat ttttttatgc ctcggtaatg attttcattt
      361 ttttttttcc acctagcgga tgactctttt tttttcttag cgattggcat tatcacataa
      421 tgaattatac attatataaa gtaatgtgat ttcttcgaag aatatactaa aaaatgagca
      481 ggcaagataa acgaaggcaa agatgacaga gcagaaagcc ctagtaaagc gtattacaaa
      541 tgaaaccaag attcagattg cgatctcttt aaagggtggt cccctagcga tagagcactc
      601 gatcttccca gaaaaagagg cagaagcagt agcagaacag gccacacaat cgcaagtgat
      661 taacgtccac acaggtatag ggtttctgga ccatatgata catgctctgg ccaagcattc
      721 cggctggtcg ctaatcgttg agtgcattgg tgacttacac atagacgacc atcacaccac
      781 tgaagactgc gggattgctc tcggtcaagc ttttaaagag gccctagggg ccgtgcgtgg
      841 agtaaaaagg tttggatcag gatttgcgcc tttggatgag gcactttcca gagcggtggt
      901 agatctttcg aacaggccgt acgcagttgt cgaacttggt ttgcaaaggg agaaagtagg
      961 agatctctct tgcgagatga tcccgcattt tcttgaaagc tttgcagagg ctagcagaat
     1021 taccctccac gttgattgtc tgcgaggcaa gaatgatcat caccgtagtg agagtgcgtt
     1081 caaggctctt gcggttgcca taagagaagc cacctcgccc aatggtacca acgatgttcc
     1141 ctccaccaaa ggtgttctta tgtagtgaca ccgattattt aaagctgcag catacgatat
     1201 atatacatgt gtatatatgt atacctatga atgtcagtaa gtatgtatac gaacagtatg
     1261 atactgaaga tgacaaggta atgcatcatt ctatacgtgt cattctgaac gaggcgcgct
     1321 ttcctttttt ctttttgctt tttctttttt tttctcttga actcgacgcg tcgaggaaaa
     1381 ctcttcaata gttttgccag cggaattcca cttgcaatta cataaaaaat tccggcggtt
     1441 tttcgcgtgt gactcaatgt cgaaatacct gcctaatgaa catgaacatc gcccaaatgt
     1501 atttgaagac ccgctgggag aagttcaaga tatataagta acaagcagcc aatagtataa
     1561 aaaaaaatct gagtttatta cctttcctgg aatttcagtg aaaaactgct aattatagag
     1621 agatatcaca gagttactca ctaatgacta acgaaaaggt ctggatagag aagttggata
     1681 atccaactct ttcagtgtta ccacatgact ttttacgccc acaacaagaa ccttatacga
     1741 aacaagctac atattcgtta cagctacctc agctcgatgt gcctcatgat agtttttcta
     1801 acaaatacgc tgtcgctttg agtgtatggg ctgcattgat atatagagta accggtgacg
     1861 atgatattgt tctttatatt gcgaataaca aaatcttaag attcaatatt caaccaacgt
     1921 ggtcatttaa tgagctgtat tctacaatta acaatgagtt gaacaagctc aattctattg
     1981 aggccaattt ttcctttgac gagctagctg aaaaaattca aagttgccaa gatctggaaa
     2041 ggacccctca gttgttccgt ttggcctttt tggaaaacca agatttcaaa ttagacgagt
     2101 tcaagcatca tttagtggac tttgctttga atttggatac cagtaataat gcgcatgttt
     2161 tgaacttaat ttataacagc ttactgtatt cgaatgaaag agtaaccatt gttgcggacc
     2221 aatttactca atatttgact gctgcgctaa gcgatccatc caattgcata actaaaatct
     2281 ctctgatcac cgcatcatcc aaggatagtt tacctgatcc aactaagaac ttgggctggt
     2341 gcgatttcgt ggggtgtatt cacgacattt tccaggacaa tgctgaagcc ttcccagaga
     2401 gaacctgtgt tgtggagact ccaacactaa attccgacaa gtcccgttct ttcacttatc
     2461 gcgacatcaa ccgcacttct aacatagttg cccattattt gattaaaaca ggtatcaaaa
     2521 gaggtgatgt agtgatgatc tattcttcta ggggtgtgga tttgatggta tgtgtgatgg
     2581 gtgtcttgaa agccggcgca accttttcag ttatcgaccc tgcatatccc ccagccagac
     2641 aaaccattta cttaggtgtt gctaaaccac gtgggttgat tgttattaga gctgctggac
     2701 aattggatca actagtagaa gattacatca atgatgaatt ggagattgtt tcaagaatca
     2761 attccatcgc tattcaagaa aatggtacca ttgaaggtgg caaattggac aatggcgagg
     2821 atgttttggc tccatatgat cactacaaag acaccagaac aggtgttgta gttggaccag
     2881 attccaaccc aaccctatct ttcacatctg gttccgaagg tattcctaag ggtgttcttg
     2941 gtagacattt ttccttggct tattatttca attggatgtc caaaaggttc aacttaacag
     3001 aaaatgataa attcacaatg ctgagcggta ttgcacatga tccaattcaa agagatatgt
     3061 ttacaccatt atttttaggt gcccaattgt atgtccctac tcaagatgat attggtacac
     3121 cgggccgttt agcggaatgg atgagtaagt atggttgcac agttacccat ttaacacctg
     3181 ccatgggtca attacttact gcccaagcta ctacaccatt ccctaagtta catcatgcgt
     3241 tctttgtggg tgacatttta acaaaacgtg attgtctgag gttacaaacc ttggcagaaa
     3301 attgccgtat tgttaatatg tacggtacca ctgaaacaca gcgtgcagtt tcttatttcg
     3361 aagttaaatc aaaaaatgac gatccaaact ttttgaaaaa attgaaagat gtcatgcctg
     3421 ctggtaaagg tatgttgaac gttcagctac tagttgttaa caggaacgat cgtactcaaa
     3481 tatgtggtat tggcgaaata ggtgagattt atgttcgtgc aggtggtttg gccgaaggtt
     3541 atagaggatt accagaattg aataaagaaa aatttgtgaa caactggttt gttgaaaaag
     3601 atcactggaa ttatttggat aaggataatg gtgaaccttg gagacaattc tggttaggtc
     3661 caagagatag attgtacaga acgggtgatt taggtcgtta tctaccaaac ggtgactgtg
     3721 aatgttgcgg tagggctgat gatcaagtta aaattcgtgg gttcagaatc gaattaggag
     3781 aaatagatac gcacatttcc caacatccat tggtaagaga aaacattact ttagttcgca
     3841 aaaatgccga caatgagcca acattgatca catttatggt cccaagattt gacaagccag
     3901 atgacttgtc taagttccaa agtgatgttc caaaggaggt tgaaactgac cctatagtta
     3961 agggcttaat cggttaccat cttttatcca aggacatcag gactttctta aagaaaagat
     4021 tggctagcta tgctatgcct tccttgattg tggttatgga taaactacca ttgaatccaa
     4081 atggtaaagt tgataagcct aaacttcaat tcccaactcc caagcaatta aatttggtag
     4141 ctgaaaatac agtttctgaa actgacgact ctcagtttac caatgttgag cgcgaggtta
     4201 gagacttatg gttaagtata ttacctacca agccagcatc tgtatcacca gatgattcgt
     4261 ttttcgattt aggtggtcat tctatcttgg ctaccaaaat gatttttacc ttaaagaaaa
     4321 agctgcaagt tgatttacca ttgggcacaa ttttcaagta tccaacgata aaggcctttg
     4381 ccgcggaaat tgacagaatt aaatcatcgg gtggatcatc tcaaggtgag gtcgtcgaaa
     4441 atgtcactgc aaattatgcg gaagacgcca agaaattggt tgagacgcta ccaagttcgt
     4501 acccctctcg agaatatttt gttgaaccta atagtgccga aggaaaaaca acaattaatg
     4561 tgtttgttac cggtgtcaca ggatttctgg gctcctacat ccttgcagat ttgttaggac
     4621 gttctccaaa gaactacagt ttcaaagtgt ttgcccacgt cagggccaag gatgaagaag
     4681 ctgcatttgc aagattacaa aaggcaggta tcacctatgg tacttggaac gaaaaatttg
     4741 cctcaaatat taaagttgta ttaggcgatt tatctaaaag ccaatttggt ctttcagatg
     4801 agaagtggat ggatttggca aacacagttg atataattat ccataatggt gcgttagttc
     4861 actgggttta tccatatgcc aaattgaggg atccaaatgt tatttcaact atcaatgtta
     4921 tgagcttagc cgccgtcggc aagccaaagt tctttgactt tgtttcctcc acttctactc
     4981 ttgacactga atactacttt aatttgtcag ataaacttgt tagcgaaggg aagccaggca
     5041 ttttagaatc agacgattta atgaactctg caagcgggct cactggtgga tatggtcagt
     5101 ccaaatgggc tgctgagtac atcattagac gtgcaggtga aaggggccta cgtgggtgta
     5161 ttgtcagacc aggttacgta acaggtgcct ctgccaatgg ttcttcaaac acagatgatt
     5221 tcttattgag atttttgaaa ggttcagtcc aattaggtaa gattccagat atcgaaaatt
     5281 ccgtgaatat ggttccagta gatcatgttg ctcgtgttgt tgttgctacg tctttgaatc
     5341 ctcccaaaga aaatgaattg gccgttgctc aagtaacggg tcacccaaga atattattca
     5401 aagactactt gtatacttta cacgattatg gttacgatgt cgaaatcgaa agctattcta
     5461 aatggaagaa atcattggag gcgtctgtta ttgacaggaa tgaagaaaat gcgttgtatc
     5521 ctttgctaca catggtctta gacaacttac ctgaaagtac caaagctccg gaactagacg
     5581 ataggaacgc cgtggcatct ttaaagaaag acaccgcatg gacaggtgtt gattggtcta
     5641 atggaatagg tgttactcca gaagaggttg gtatatatat tgcattttta aacaaggttg
     5701 gatttttacc tccaccaact cataatgaca aacttccact gccaagtata gaactaactc
     5761 aagcgcaaat aagtctagtt gcttcaggtg ctggtgctcg tggaagctcc gcagcagctt
     5821 aaggttgagc attacgtatg atatgtgcgc gccatcgagg agaacttcta gtatatctac
     5881 atacctaata ttattgcctt attaaaaatg gaatcccaac aattacatca aaatccacat
     5941 tctcttcaaa atcaattgtc ctgtacttcc ttgttcatgt gtgttcaaaa acgttatatt
     6001 tataggataa ttatactcta tttctcaaca agtaattggt tgtttggccg agcggtctaa
     6061 ggcgcctgat tcaagaaata tcttgaccgc agttaactgt gggaatactc aggtatcgta
     6121 agatgcaaga gttcgaatct cttagcaacc attatttttt tcctcaacat aacgagaaca
     6181 cacaggggcg ctatcgcaca gaatcaaatt cgatgactgg aaattttttg ttaatttcag
     6241 aggtcgcctg acgcatatac ctttttcaac tgaaaaattg ggagaaaaag gaaaggtgag
     6301 agcgccggaa ccggcttttc atatagaata gagaagcgtt catgactaaa tgcttgcatc
     6361 acaatacttg aagttgacaa tattatttaa ggacctattg ttttttccaa taggtggtta
     6421 gcaatcgtct tactttctaa cttttcttac cttttacatt tcagcaatat atatatatat
     6481 atttcaagga tataccattc taatgtctgc ccctaagaag atcgtcgttt tgccaggtga
     6541 ccacgttggt caagaaatca cagccgaagc cattaaggtt cttaaagcta tttctgatgt
     6601 tcgttccaat gtcaagttcg atttcgaaaa tcatttaatt ggtggtgctg ctatcgatgc
     6661 tacaggtgtt ccacttccag atgaggcgct ggaagcctcc aagaaggctg atgccgtttt
     6721 gttaggtgct gtgggtggtc ctaaatgggg taccggtagt gttagacctg aacaaggttt
     6781 actaaaaatc cgtaaagaac ttcaattgta cgccaactta agaccatgta actttgcatc
     6841 cgactctctt ttagacttat ctccaatcaa gccacaattt gctaaaggta ctgacttcgt
     6901 tgttgtcaga gaattagtgg gaggtattta ctttggtaag agaaaggaag acgatggtga
     6961 tggtgtcgct tgggatagtg aacaatacac cgttccagaa gtgcaaagaa tcacaagaat
     7021 ggccgctttc atggccctac aacatgagcc accattgcct atttggtcct tggataaagc
     7081 taatgttttg gcctcttcaa gattatggag aaaaactgtg gaggaaacca tcaagaacga
     7141 attccctaca ttgaaggttc aacatcaatt gattgattct gccgccatga tcctagttaa
     7201 gaacccaacc cacctaaatg gtattataat caccagcaac atgtttggtg atatcatctc
     7261 cgatgaagcc tccgttatcc caggttcctt gggtttgttg ccatctgcgt ccttggcctc
     7321 tttgccagac aagaacaccg catttggttt gtacgaacca tgccacggtt ctgctccaga
     7381 tttgccaaag aataaggtca accctatcgc cactatcttg tctgctgcaa tgatgttgaa
     7441 attgtcattg aacttgcctg aagaaggtaa ggccattgaa gatgcagtta aaaaggtttt
     7501 ggatgcaggt atcagaactg gtgatttagg tggttccaac agtaccaccg aagtcggtga
     7561 tgctgtcgcc gaagaagtta agaaaatcct tgcttaaaaa gattctcttt ttttatgata
     7621 tttgtacata aactttataa atgaaattca taatagaaac gacacgaaat tacaaaatgg
     7681 aatatgttca tagggtagac gaaactatat acgcaatcta catacattta tcaagaagga
     7741 gaaaaaggag gatgtaaagg aatacaggta agcaaattga tactaatggc tcaacgtgat
     7801 aaggaaaaag aattgcactt taacattaat attgacaagg aggagggcac cacacaaaaa
     7861 gttaggtgta acagaaaatc atgaaactat gattcctaat ttatatattg gaggattttc
     7921 tctaaaaaaa aaaaaataca acaaataaaa aacactcaat gacctgacca tttgatggag
     7981 tttaagtcaa taccttcttg aaccatttcc cataatggtg aaagttccct caagaatttt
     8041 actctgtcag aaacggcctt acgacgtagt cgaggatctc ctaggttaat taagtttaaa
     8101 cagatcctat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcagga
     8161 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     8221 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     8281 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     8341 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
     8401 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     8461 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     8521 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     8581 acccgccgcg cttaatgcgc cgctacaggg cgcgtccatt cgccattcag gctgcgcaac
     8641 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
     8701 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
     8761 acgacggcca gtgaattgta atacgactca ctatagggcg aattggagct ccaccgcggt
     8821 ggcggccgct ctagaactag tgggtccccc gggctgcagg aattcgatat caagcttatc
     8881 gataccgtcg acctggaggg ggggcccggt accagctttt gttcccttta gtgagggtta
     8941 atttcgagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     9001 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     9061 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     9121 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     9181 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     9241 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     9301 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     9361 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     9421 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     9481 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     9541 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     9601 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     9661 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     9721 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     9781 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     9841 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     9901 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     9961 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
    10021 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
    10081 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
    10141 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactgccc
    10201 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
    10261 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
    10321 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
    10381 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
    10441 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
    10501 cgatcaaggc gagttacatg atcccccatg ttgtgaaaaa aagcggttag ctccttcggt
    10561 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    10621 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    10681 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    10741 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    10801 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    10861 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    10921 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    10981 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    11041 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    11101 cgaaaagtgc cacctatcgc ttgcctgtaa cttacacgcg cctcgtatct tttaatgatg
    11161 gaataatttg ggaatttact ctgtgtttat ttatttttat gttttgtatt tggattttag
    11221 aaagtaaata aagaaggtag aagagttacg gaatgaagaa aaaaaaataa acaaaggttt
    11281 aaaaaatttc aacaaaaagc gtactttaca tatatattta ttagacaaga aaagcagatt
    11341 aaatagatat acattcgatt aacgataagt aaaatgtaaa atcacaggat tttcgtgtgt
    11401 ggtcttctac acagacaaga tgaaacaatt cggcattaat acctgagagc aggaagagca
    11461 agataaaagg tagtatttgt tggcgatccc cctagagtct tttacatctt cggaaaacaa
    11521 aaactatttt ttctttaatt tcttttttta ctttctattt ttaatttata tatttatatt
    11581 aaaaaattta aattataatt atttttatag cacgtgatga aaaggacccc cgtcctaaga
    11641 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtc
//
