LOCUS       sequence-107499-       10482 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  harbours marker genes HIS3, URA3 and LYS2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10482)
  AUTHORS   Mulleder M, Campbell K, Matsarskaia O, Eckerstorfer F, Ralser M
  TITLE     Saccharomyces cerevisiae single-copy plasmids for auxotrophy 
            compensation, multiple marker selection, and for designing 
            metabolically cooperating communities.
  JOURNAL   F1000Res. 2016 Sep 20;5:2351. eCollection 2016.
  PUBMED    27830062
REFERENCE   2  (bases 1 to 10482)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "F1000Res.";
            date: "2016-09-20"; volume: "5"; pages: "2351. eCollection 2016"
COMMENT     Sequence Label: pHUK
FEATURES             Location/Qualifiers
     source          1..10482
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        315..502
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             503..1165
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        1404..1643
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
     CDS             1644..5822
                     /codon_start=1
                     /gene="S. cerevisiae LYS2"
                     /product="alpha-aminoadipate reductase, required for lysine
                     biosynthesis"
                     /label=LYS2
                     /note="yeast auxotrophic marker"
                     /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD
                     VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN
                     ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN
                     LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS
                     LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI
                     VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA
                     KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD
                     HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT
                     MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL
                     LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS
                     KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG
                     LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC
                     CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD
                     DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP
                     NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD
                     SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV
                     VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD
                     LLGRSPKNYSFKVFAHVRAKDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQF
                     GLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDFV
                     SSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMNSASGLTGGYGQSKWAAEYIIRRAGE
                     RGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVARV
                     VVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVID
                     RNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEVG
                     IYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA"
     CDS             6009..6812
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(6943..7398)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7080..7101)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7292..7311
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7524..7546
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7538..7555
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7539..7555
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7562..7581
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7562..7580
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    7589..7696
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(7708..7726)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7747..7763)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7747..7763)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7760..7782)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7771..7787
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7795..7801,7802..7819,7820..7825))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7840..7861
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7978..7995)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8149..8737)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8229..8248)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8908..9699,9700..9768))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9531..9550
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9769..9873)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10455..10473
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcggacg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataattc cgttttaaga gcttggtgag cgctaggagt cactgccagg tatcgtttga
      241 acacggcatt agtcagggaa gtcataacac agtcctttcc cgcaattttc tttttctatt
      301 actcttggcc tcctctagta cactctatat ttttttatgc ctcggtaatg attttcattt
      361 ttttttttcc acctagcgga tgactctttt tttttcttag cgattggcat tatcacataa
      421 tgaattatac attatataaa gtaatgtgat ttcttcgaag aatatactaa aaaatgagca
      481 ggcaagataa acgaaggcaa agatgacaga gcagaaagcc ctagtaaagc gtattacaaa
      541 tgaaaccaag attcagattg cgatctcttt aaagggtggt cccctagcga tagagcactc
      601 gatcttccca gaaaaagagg cagaagcagt agcagaacag gccacacaat cgcaagtgat
      661 taacgtccac acaggtatag ggtttctgga ccatatgata catgctctgg ccaagcattc
      721 cggctggtcg ctaatcgttg agtgcattgg tgacttacac atagacgacc atcacaccac
      781 tgaagactgc gggattgctc tcggtcaagc ttttaaagag gccctagggg ccgtgcgtgg
      841 agtaaaaagg tttggatcag gatttgcgcc tttggatgag gcactttcca gagcggtggt
      901 agatctttcg aacaggccgt acgcagttgt cgaacttggt ttgcaaaggg agaaagtagg
      961 agatctctct tgcgagatga tcccgcattt tcttgaaagc tttgcagagg ctagcagaat
     1021 taccctccac gttgattgtc tgcgaggcaa gaatgatcat caccgtagtg agagtgcgtt
     1081 caaggctctt gcggttgcca taagagaagc cacctcgccc aatggtacca acgatgttcc
     1141 ctccaccaaa ggtgttctta tgtagtgaca ccgattattt aaagctgcag catacgatat
     1201 atatacatgt gtatatatgt atacctatga atgtcagtaa gtatgtatac gaacagtatg
     1261 atactgaaga tgacaaggta atgcatcatt ctatacgtgt cattctgaac gaggcgcgct
     1321 ttcctttttt ctttttgctt tttctttttt tttctcttga actcgacgcg tcgaggaaaa
     1381 ctcttcaata gttttgccag cggaattcca cttgcaatta cataaaaaat tccggcggtt
     1441 tttcgcgtgt gactcaatgt cgaaatacct gcctaatgaa catgaacatc gcccaaatgt
     1501 atttgaagac ccgctgggag aagttcaaga tatataagta acaagcagcc aatagtataa
     1561 aaaaaaatct gagtttatta cctttcctgg aatttcagtg aaaaactgct aattatagag
     1621 agatatcaca gagttactca ctaatgacta acgaaaaggt ctggatagag aagttggata
     1681 atccaactct ttcagtgtta ccacatgact ttttacgccc acaacaagaa ccttatacga
     1741 aacaagctac atattcgtta cagctacctc agctcgatgt gcctcatgat agtttttcta
     1801 acaaatacgc tgtcgctttg agtgtatggg ctgcattgat atatagagta accggtgacg
     1861 atgatattgt tctttatatt gcgaataaca aaatcttaag attcaatatt caaccaacgt
     1921 ggtcatttaa tgagctgtat tctacaatta acaatgagtt gaacaagctc aattctattg
     1981 aggccaattt ttcctttgac gagctagctg aaaaaattca aagttgccaa gatctggaaa
     2041 ggacccctca gttgttccgt ttggcctttt tggaaaacca agatttcaaa ttagacgagt
     2101 tcaagcatca tttagtggac tttgctttga atttggatac cagtaataat gcgcatgttt
     2161 tgaacttaat ttataacagc ttactgtatt cgaatgaaag agtaaccatt gttgcggacc
     2221 aatttactca atatttgact gctgcgctaa gcgatccatc caattgcata actaaaatct
     2281 ctctgatcac cgcatcatcc aaggatagtt tacctgatcc aactaagaac ttgggctggt
     2341 gcgatttcgt ggggtgtatt cacgacattt tccaggacaa tgctgaagcc ttcccagaga
     2401 gaacctgtgt tgtggagact ccaacactaa attccgacaa gtcccgttct ttcacttatc
     2461 gcgacatcaa ccgcacttct aacatagttg cccattattt gattaaaaca ggtatcaaaa
     2521 gaggtgatgt agtgatgatc tattcttcta ggggtgtgga tttgatggta tgtgtgatgg
     2581 gtgtcttgaa agccggcgca accttttcag ttatcgaccc tgcatatccc ccagccagac
     2641 aaaccattta cttaggtgtt gctaaaccac gtgggttgat tgttattaga gctgctggac
     2701 aattggatca actagtagaa gattacatca atgatgaatt ggagattgtt tcaagaatca
     2761 attccatcgc tattcaagaa aatggtacca ttgaaggtgg caaattggac aatggcgagg
     2821 atgttttggc tccatatgat cactacaaag acaccagaac aggtgttgta gttggaccag
     2881 attccaaccc aaccctatct ttcacatctg gttccgaagg tattcctaag ggtgttcttg
     2941 gtagacattt ttccttggct tattatttca attggatgtc caaaaggttc aacttaacag
     3001 aaaatgataa attcacaatg ctgagcggta ttgcacatga tccaattcaa agagatatgt
     3061 ttacaccatt atttttaggt gcccaattgt atgtccctac tcaagatgat attggtacac
     3121 cgggccgttt agcggaatgg atgagtaagt atggttgcac agttacccat ttaacacctg
     3181 ccatgggtca attacttact gcccaagcta ctacaccatt ccctaagtta catcatgcgt
     3241 tctttgtggg tgacatttta acaaaacgtg attgtctgag gttacaaacc ttggcagaaa
     3301 attgccgtat tgttaatatg tacggtacca ctgaaacaca gcgtgcagtt tcttatttcg
     3361 aagttaaatc aaaaaatgac gatccaaact ttttgaaaaa attgaaagat gtcatgcctg
     3421 ctggtaaagg tatgttgaac gttcagctac tagttgttaa caggaacgat cgtactcaaa
     3481 tatgtggtat tggcgaaata ggtgagattt atgttcgtgc aggtggtttg gccgaaggtt
     3541 atagaggatt accagaattg aataaagaaa aatttgtgaa caactggttt gttgaaaaag
     3601 atcactggaa ttatttggat aaggataatg gtgaaccttg gagacaattc tggttaggtc
     3661 caagagatag attgtacaga acgggtgatt taggtcgtta tctaccaaac ggtgactgtg
     3721 aatgttgcgg tagggctgat gatcaagtta aaattcgtgg gttcagaatc gaattaggag
     3781 aaatagatac gcacatttcc caacatccat tggtaagaga aaacattact ttagttcgca
     3841 aaaatgccga caatgagcca acattgatca catttatggt cccaagattt gacaagccag
     3901 atgacttgtc taagttccaa agtgatgttc caaaggaggt tgaaactgac cctatagtta
     3961 agggcttaat cggttaccat cttttatcca aggacatcag gactttctta aagaaaagat
     4021 tggctagcta tgctatgcct tccttgattg tggttatgga taaactacca ttgaatccaa
     4081 atggtaaagt tgataagcct aaacttcaat tcccaactcc caagcaatta aatttggtag
     4141 ctgaaaatac agtttctgaa actgacgact ctcagtttac caatgttgag cgcgaggtta
     4201 gagacttatg gttaagtata ttacctacca agccagcatc tgtatcacca gatgattcgt
     4261 ttttcgattt aggtggtcat tctatcttgg ctaccaaaat gatttttacc ttaaagaaaa
     4321 agctgcaagt tgatttacca ttgggcacaa ttttcaagta tccaacgata aaggcctttg
     4381 ccgcggaaat tgacagaatt aaatcatcgg gtggatcatc tcaaggtgag gtcgtcgaaa
     4441 atgtcactgc aaattatgcg gaagacgcca agaaattggt tgagacgcta ccaagttcgt
     4501 acccctctcg agaatatttt gttgaaccta atagtgccga aggaaaaaca acaattaatg
     4561 tgtttgttac cggtgtcaca ggatttctgg gctcctacat ccttgcagat ttgttaggac
     4621 gttctccaaa gaactacagt ttcaaagtgt ttgcccacgt cagggccaag gatgaagaag
     4681 ctgcatttgc aagattacaa aaggcaggta tcacctatgg tacttggaac gaaaaatttg
     4741 cctcaaatat taaagttgta ttaggcgatt tatctaaaag ccaatttggt ctttcagatg
     4801 agaagtggat ggatttggca aacacagttg atataattat ccataatggt gcgttagttc
     4861 actgggttta tccatatgcc aaattgaggg atccaaatgt tatttcaact atcaatgtta
     4921 tgagcttagc cgccgtcggc aagccaaagt tctttgactt tgtttcctcc acttctactc
     4981 ttgacactga atactacttt aatttgtcag ataaacttgt tagcgaaggg aagccaggca
     5041 ttttagaatc agacgattta atgaactctg caagcgggct cactggtgga tatggtcagt
     5101 ccaaatgggc tgctgagtac atcattagac gtgcaggtga aaggggccta cgtgggtgta
     5161 ttgtcagacc aggttacgta acaggtgcct ctgccaatgg ttcttcaaac acagatgatt
     5221 tcttattgag atttttgaaa ggttcagtcc aattaggtaa gattccagat atcgaaaatt
     5281 ccgtgaatat ggttccagta gatcatgttg ctcgtgttgt tgttgctacg tctttgaatc
     5341 ctcccaaaga aaatgaattg gccgttgctc aagtaacggg tcacccaaga atattattca
     5401 aagactactt gtatacttta cacgattatg gttacgatgt cgaaatcgaa agctattcta
     5461 aatggaagaa atcattggag gcgtctgtta ttgacaggaa tgaagaaaat gcgttgtatc
     5521 ctttgctaca catggtctta gacaacttac ctgaaagtac caaagctccg gaactagacg
     5581 ataggaacgc cgtggcatct ttaaagaaag acaccgcatg gacaggtgtt gattggtcta
     5641 atggaatagg tgttactcca gaagaggttg gtatatatat tgcattttta aacaaggttg
     5701 gatttttacc tccaccaact cataatgaca aacttccact gccaagtata gaactaactc
     5761 aagcgcaaat aagtctagtt gcttcaggtg ctggtgctcg tggaagctcc gcagcagctt
     5821 aaggttgagc attacgtatg atatgtgcat gcgattcggt aatctccgaa cagaaggaag
     5881 aacgaaggaa ggagcacaga cttagattgg tatatatacg catatgtagt gttgaagaaa
     5941 catgaaattg cccagtattc ttaacccaac tgcacagaac aaaaacctgc aggaaacgaa
     6001 gataaatcat gtcgaaagct acatataagg aacgtgctgc tactcatcct agtcctgttg
     6061 ctgccaagct atttaatatc atgcacgaaa agcaaacaaa cttgtgtgct tcattggatg
     6121 ttcgtaccac caaggaatta ctggagttag ttgaagcatt aggtcccaaa atttgtttac
     6181 taaaaacaca tgtggatatc ttgactgatt tttccatgga gggcacagtt aagccgctaa
     6241 aggcattatc cgccaagtac aattttttac tcttcgaaga cagaaaattt gctgacattg
     6301 gtaatacagt caaattgcag tactctgcgg gtgtatacag aatagcagaa tgggcagaca
     6361 ttacgaatgc acacggtgtg gtgggcccag gtattgttag cggtttgaag caggcggcag
     6421 aagaagtaac aaaggaacct agaggccttt tgatgttagc agaattgtca tgcaagggct
     6481 ccctatctac tggagaatat actaagggta ctgttgacat tgcgaagagc gacaaagatt
     6541 ttgttatcgg ctttattgct caaagagaca tgggtggaag agatgaaggt tacgattggt
     6601 tgattatgac acccggtgtg ggtttagatg acaagggaga cgcattgggt caacagtata
     6661 gaaccgtgga tgatgtggtc tctacaggat ctgacattat tattgttgga agaggactat
     6721 ttgcaaaggg aagggatgct aaggtagagg gtgaacgtta cagaaaagca ggctgggaag
     6781 catatttgag aagatgcggc cagcaaaact aaaaaactgt attataagta aatgcatgta
     6841 tactaaactc acaaattaga gcttcaattt aattatatca gttattaccc tatgcggtgt
     6901 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta agcgttaata
     6961 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     7021 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     7081 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     7141 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     7201 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     7261 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     7321 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     7381 cgccgctaca gggcgcgtcc attcgccatt caggctgcgc aactgttggg aagggcgatc
     7441 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
     7501 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt
     7561 gtaatacgac tcactatagg gcgaattgga gctccaccgc ggtggcggcc gctctagaac
     7621 tagtgggtcc cccgggctgc aggaattcga tatcaagctt atcgataccg tcgacctgga
     7681 gggggggccc ggtaccagct tttgttccct ttagtgaggg ttaatttcga gcttggcgta
     7741 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     7801 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     7861 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     7921 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     7981 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     8041 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     8101 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     8161 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     8221 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     8281 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     8341 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     8401 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     8461 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     8521 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     8581 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     8641 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     8701 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     8761 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     8821 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     8881 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     8941 agcgatctgt ctatttcgtt catccatagt tgcctgactg cccgtcgtgt agataactac
     9001 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     9061 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     9121 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     9181 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     9241 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     9301 atgatccccc atgttgtgaa aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     9361 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     9421 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     9481 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     9541 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     9601 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     9661 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     9721 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     9781 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     9841 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctat
     9901 cgcttgcctg taacttacac gcgcctcgta tcttttaatg atggaataat ttgggaattt
     9961 actctgtgtt tatttatttt tatgttttgt atttggattt tagaaagtaa ataaagaagg
    10021 tagaagagtt acggaatgaa gaaaaaaaaa taaacaaagg tttaaaaaat ttcaacaaaa
    10081 agcgtacttt acatatatat ttattagaca agaaaagcag attaaataga tatacattcg
    10141 attaacgata agtaaaatgt aaaatcacag gattttcgtg tgtggtcttc tacacagaca
    10201 agatgaaaca attcggcatt aatacctgag agcaggaaga gcaagataaa aggtagtatt
    10261 tgttggcgat ccccctagag tcttttacat cttcggaaaa caaaaactat tttttcttta
    10321 atttcttttt ttactttcta tttttaattt atatatttat attaaaaaat ttaaattata
    10381 attattttta tagcacgtga tgaaaaggac ccccgtccta agaaaccatt attatcatga
    10441 cattaaccta taaaaatagg cgtatcacga ggccctttcg tc
//
