LOCUS       sequence-107501-        9449 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  harbours marker genes HIS3 and LYS2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9449)
  AUTHORS   Mulleder M, Campbell K, Matsarskaia O, Eckerstorfer F, Ralser M
  TITLE     Saccharomyces cerevisiae single-copy plasmids for auxotrophy 
            compensation, multiple marker selection, and for designing 
            metabolically cooperating communities.
  JOURNAL   F1000Res. 2016 Sep 20;5:2351. eCollection 2016.
  PUBMED    27830062
REFERENCE   2  (bases 1 to 9449)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "F1000Res.";
            date: "2016-09-20"; volume: "5"; pages: "2351. eCollection 2016"
COMMENT     Sequence Label: pHK
FEATURES             Location/Qualifiers
     source          1..9449
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        315..502
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             503..1165
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        1404..1643
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
     CDS             1644..5822
                     /codon_start=1
                     /gene="S. cerevisiae LYS2"
                     /product="alpha-aminoadipate reductase, required for lysine
                     biosynthesis"
                     /label=LYS2
                     /note="yeast auxotrophic marker"
                     /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD
                     VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN
                     ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN
                     LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS
                     LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI
                     VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA
                     KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD
                     HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT
                     MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL
                     LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS
                     KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG
                     LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC
                     CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD
                     DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP
                     NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD
                     SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV
                     VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD
                     LLGRSPKNYSFKVFAHVRAKDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQF
                     GLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDFV
                     SSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMNSASGLTGGYGQSKWAAEYIIRRAGE
                     RGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVARV
                     VVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVID
                     RNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEVG
                     IYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA"
     rep_origin      complement(5910..6365)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6047..6068)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6259..6278
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6491..6513
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6505..6522
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6506..6522
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6529..6548
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6529..6547
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    6556..6663
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(6675..6693)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6714..6730)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6714..6730)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6727..6749)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6738..6754
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6762..6768,6769..6786,6787..6792))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6807..6828
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6945..6962)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7116..7704)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7196..7215)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7875..8666,8667..8735))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8498..8517
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8736..8840)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9422..9440
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcggacg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataattc cgttttaaga gcttggtgag cgctaggagt cactgccagg tatcgtttga
      241 acacggcatt agtcagggaa gtcataacac agtcctttcc cgcaattttc tttttctatt
      301 actcttggcc tcctctagta cactctatat ttttttatgc ctcggtaatg attttcattt
      361 ttttttttcc acctagcgga tgactctttt tttttcttag cgattggcat tatcacataa
      421 tgaattatac attatataaa gtaatgtgat ttcttcgaag aatatactaa aaaatgagca
      481 ggcaagataa acgaaggcaa agatgacaga gcagaaagcc ctagtaaagc gtattacaaa
      541 tgaaaccaag attcagattg cgatctcttt aaagggtggt cccctagcga tagagcactc
      601 gatcttccca gaaaaagagg cagaagcagt agcagaacag gccacacaat cgcaagtgat
      661 taacgtccac acaggtatag ggtttctgga ccatatgata catgctctgg ccaagcattc
      721 cggctggtcg ctaatcgttg agtgcattgg tgacttacac atagacgacc atcacaccac
      781 tgaagactgc gggattgctc tcggtcaagc ttttaaagag gccctagggg ccgtgcgtgg
      841 agtaaaaagg tttggatcag gatttgcgcc tttggatgag gcactttcca gagcggtggt
      901 agatctttcg aacaggccgt acgcagttgt cgaacttggt ttgcaaaggg agaaagtagg
      961 agatctctct tgcgagatga tcccgcattt tcttgaaagc tttgcagagg ctagcagaat
     1021 taccctccac gttgattgtc tgcgaggcaa gaatgatcat caccgtagtg agagtgcgtt
     1081 caaggctctt gcggttgcca taagagaagc cacctcgccc aatggtacca acgatgttcc
     1141 ctccaccaaa ggtgttctta tgtagtgaca ccgattattt aaagctgcag catacgatat
     1201 atatacatgt gtatatatgt atacctatga atgtcagtaa gtatgtatac gaacagtatg
     1261 atactgaaga tgacaaggta atgcatcatt ctatacgtgt cattctgaac gaggcgcgct
     1321 ttcctttttt ctttttgctt tttctttttt tttctcttga actcgacgcg tcgaggaaaa
     1381 ctcttcaata gttttgccag cggaattcca cttgcaatta cataaaaaat tccggcggtt
     1441 tttcgcgtgt gactcaatgt cgaaatacct gcctaatgaa catgaacatc gcccaaatgt
     1501 atttgaagac ccgctgggag aagttcaaga tatataagta acaagcagcc aatagtataa
     1561 aaaaaaatct gagtttatta cctttcctgg aatttcagtg aaaaactgct aattatagag
     1621 agatatcaca gagttactca ctaatgacta acgaaaaggt ctggatagag aagttggata
     1681 atccaactct ttcagtgtta ccacatgact ttttacgccc acaacaagaa ccttatacga
     1741 aacaagctac atattcgtta cagctacctc agctcgatgt gcctcatgat agtttttcta
     1801 acaaatacgc tgtcgctttg agtgtatggg ctgcattgat atatagagta accggtgacg
     1861 atgatattgt tctttatatt gcgaataaca aaatcttaag attcaatatt caaccaacgt
     1921 ggtcatttaa tgagctgtat tctacaatta acaatgagtt gaacaagctc aattctattg
     1981 aggccaattt ttcctttgac gagctagctg aaaaaattca aagttgccaa gatctggaaa
     2041 ggacccctca gttgttccgt ttggcctttt tggaaaacca agatttcaaa ttagacgagt
     2101 tcaagcatca tttagtggac tttgctttga atttggatac cagtaataat gcgcatgttt
     2161 tgaacttaat ttataacagc ttactgtatt cgaatgaaag agtaaccatt gttgcggacc
     2221 aatttactca atatttgact gctgcgctaa gcgatccatc caattgcata actaaaatct
     2281 ctctgatcac cgcatcatcc aaggatagtt tacctgatcc aactaagaac ttgggctggt
     2341 gcgatttcgt ggggtgtatt cacgacattt tccaggacaa tgctgaagcc ttcccagaga
     2401 gaacctgtgt tgtggagact ccaacactaa attccgacaa gtcccgttct ttcacttatc
     2461 gcgacatcaa ccgcacttct aacatagttg cccattattt gattaaaaca ggtatcaaaa
     2521 gaggtgatgt agtgatgatc tattcttcta ggggtgtgga tttgatggta tgtgtgatgg
     2581 gtgtcttgaa agccggcgca accttttcag ttatcgaccc tgcatatccc ccagccagac
     2641 aaaccattta cttaggtgtt gctaaaccac gtgggttgat tgttattaga gctgctggac
     2701 aattggatca actagtagaa gattacatca atgatgaatt ggagattgtt tcaagaatca
     2761 attccatcgc tattcaagaa aatggtacca ttgaaggtgg caaattggac aatggcgagg
     2821 atgttttggc tccatatgat cactacaaag acaccagaac aggtgttgta gttggaccag
     2881 attccaaccc aaccctatct ttcacatctg gttccgaagg tattcctaag ggtgttcttg
     2941 gtagacattt ttccttggct tattatttca attggatgtc caaaaggttc aacttaacag
     3001 aaaatgataa attcacaatg ctgagcggta ttgcacatga tccaattcaa agagatatgt
     3061 ttacaccatt atttttaggt gcccaattgt atgtccctac tcaagatgat attggtacac
     3121 cgggccgttt agcggaatgg atgagtaagt atggttgcac agttacccat ttaacacctg
     3181 ccatgggtca attacttact gcccaagcta ctacaccatt ccctaagtta catcatgcgt
     3241 tctttgtggg tgacatttta acaaaacgtg attgtctgag gttacaaacc ttggcagaaa
     3301 attgccgtat tgttaatatg tacggtacca ctgaaacaca gcgtgcagtt tcttatttcg
     3361 aagttaaatc aaaaaatgac gatccaaact ttttgaaaaa attgaaagat gtcatgcctg
     3421 ctggtaaagg tatgttgaac gttcagctac tagttgttaa caggaacgat cgtactcaaa
     3481 tatgtggtat tggcgaaata ggtgagattt atgttcgtgc aggtggtttg gccgaaggtt
     3541 atagaggatt accagaattg aataaagaaa aatttgtgaa caactggttt gttgaaaaag
     3601 atcactggaa ttatttggat aaggataatg gtgaaccttg gagacaattc tggttaggtc
     3661 caagagatag attgtacaga acgggtgatt taggtcgtta tctaccaaac ggtgactgtg
     3721 aatgttgcgg tagggctgat gatcaagtta aaattcgtgg gttcagaatc gaattaggag
     3781 aaatagatac gcacatttcc caacatccat tggtaagaga aaacattact ttagttcgca
     3841 aaaatgccga caatgagcca acattgatca catttatggt cccaagattt gacaagccag
     3901 atgacttgtc taagttccaa agtgatgttc caaaggaggt tgaaactgac cctatagtta
     3961 agggcttaat cggttaccat cttttatcca aggacatcag gactttctta aagaaaagat
     4021 tggctagcta tgctatgcct tccttgattg tggttatgga taaactacca ttgaatccaa
     4081 atggtaaagt tgataagcct aaacttcaat tcccaactcc caagcaatta aatttggtag
     4141 ctgaaaatac agtttctgaa actgacgact ctcagtttac caatgttgag cgcgaggtta
     4201 gagacttatg gttaagtata ttacctacca agccagcatc tgtatcacca gatgattcgt
     4261 ttttcgattt aggtggtcat tctatcttgg ctaccaaaat gatttttacc ttaaagaaaa
     4321 agctgcaagt tgatttacca ttgggcacaa ttttcaagta tccaacgata aaggcctttg
     4381 ccgcggaaat tgacagaatt aaatcatcgg gtggatcatc tcaaggtgag gtcgtcgaaa
     4441 atgtcactgc aaattatgcg gaagacgcca agaaattggt tgagacgcta ccaagttcgt
     4501 acccctctcg agaatatttt gttgaaccta atagtgccga aggaaaaaca acaattaatg
     4561 tgtttgttac cggtgtcaca ggatttctgg gctcctacat ccttgcagat ttgttaggac
     4621 gttctccaaa gaactacagt ttcaaagtgt ttgcccacgt cagggccaag gatgaagaag
     4681 ctgcatttgc aagattacaa aaggcaggta tcacctatgg tacttggaac gaaaaatttg
     4741 cctcaaatat taaagttgta ttaggcgatt tatctaaaag ccaatttggt ctttcagatg
     4801 agaagtggat ggatttggca aacacagttg atataattat ccataatggt gcgttagttc
     4861 actgggttta tccatatgcc aaattgaggg atccaaatgt tatttcaact atcaatgtta
     4921 tgagcttagc cgccgtcggc aagccaaagt tctttgactt tgtttcctcc acttctactc
     4981 ttgacactga atactacttt aatttgtcag ataaacttgt tagcgaaggg aagccaggca
     5041 ttttagaatc agacgattta atgaactctg caagcgggct cactggtgga tatggtcagt
     5101 ccaaatgggc tgctgagtac atcattagac gtgcaggtga aaggggccta cgtgggtgta
     5161 ttgtcagacc aggttacgta acaggtgcct ctgccaatgg ttcttcaaac acagatgatt
     5221 tcttattgag atttttgaaa ggttcagtcc aattaggtaa gattccagat atcgaaaatt
     5281 ccgtgaatat ggttccagta gatcatgttg ctcgtgttgt tgttgctacg tctttgaatc
     5341 ctcccaaaga aaatgaattg gccgttgctc aagtaacggg tcacccaaga atattattca
     5401 aagactactt gtatacttta cacgattatg gttacgatgt cgaaatcgaa agctattcta
     5461 aatggaagaa atcattggag gcgtctgtta ttgacaggaa tgaagaaaat gcgttgtatc
     5521 ctttgctaca catggtctta gacaacttac ctgaaagtac caaagctccg gaactagacg
     5581 ataggaacgc cgtggcatct ttaaagaaag acaccgcatg gacaggtgtt gattggtcta
     5641 atggaatagg tgttactcca gaagaggttg gtatatatat tgcattttta aacaaggttg
     5701 gatttttacc tccaccaact cataatgaca aacttccact gccaagtata gaactaactc
     5761 aagcgcaaat aagtctagtt gcttcaggtg ctggtgctcg tggaagctcc gcagcagctt
     5821 aaggttgagc attacgtatg atatgtgaaa cagatcctat gcggtgtgaa ataccgcaca
     5881 gatgcgtaag gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt
     5941 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     6001 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     6061 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     6121 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     6181 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     6241 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     6301 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     6361 cgcgtccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     6421 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
     6481 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaattgta atacgactca
     6541 ctatagggcg aattggagct ccaccgcggt ggcggccgct ctagaactag tgggtccccc
     6601 gggctgcagg aattcgatat caagcttatc gataccgtcg acctggaggg ggggcccggt
     6661 accagctttt gttcccttta gtgagggtta atttcgagct tggcgtaatc atggtcatag
     6721 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     6781 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     6841 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     6901 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     6961 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     7021 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     7081 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     7141 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     7201 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     7261 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     7321 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     7381 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     7441 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     7501 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     7561 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     7621 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     7681 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     7741 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     7801 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     7861 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     7921 tttcgttcat ccatagttgc ctgactgccc gtcgtgtaga taactacgat acgggagggc
     7981 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     8041 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     8101 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     8161 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     8221 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     8281 ttgtgaaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     8341 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     8401 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     8461 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     8521 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     8581 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     8641 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     8701 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     8761 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     8821 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctatcgc ttgcctgtaa
     8881 cttacacgcg cctcgtatct tttaatgatg gaataatttg ggaatttact ctgtgtttat
     8941 ttatttttat gttttgtatt tggattttag aaagtaaata aagaaggtag aagagttacg
     9001 gaatgaagaa aaaaaaataa acaaaggttt aaaaaatttc aacaaaaagc gtactttaca
     9061 tatatattta ttagacaaga aaagcagatt aaatagatat acattcgatt aacgataagt
     9121 aaaatgtaaa atcacaggat tttcgtgtgt ggtcttctac acagacaaga tgaaacaatt
     9181 cggcattaat acctgagagc aggaagagca agataaaagg tagtatttgt tggcgatccc
     9241 cctagagtct tttacatctt cggaaaacaa aaactatttt ttctttaatt tcttttttta
     9301 ctttctattt ttaatttata tatttatatt aaaaaattta aattataatt atttttatag
     9361 cacgtgatga aaaggacccc cgtcctaaga aaccattatt atcatgacat taacctataa
     9421 aaataggcgt atcacgaggc cctttcgtc
//
