LOCUS       sequence-107502-       10460 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  harbours marker genes LEU2 and LYS2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10460)
  AUTHORS   Mulleder M, Campbell K, Matsarskaia O, Eckerstorfer F, Ralser M
  TITLE     Saccharomyces cerevisiae single-copy plasmids for auxotrophy 
            compensation, multiple marker selection, and for designing 
            metabolically cooperating communities.
  JOURNAL   F1000Res. 2016 Sep 20;5:2351. eCollection 2016.
  PUBMED    27830062
REFERENCE   2  (bases 1 to 10460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "F1000Res.";
            date: "2016-09-20"; volume: "5"; pages: "2351. eCollection 2016"
COMMENT     Sequence Label: pLK
FEATURES             Location/Qualifiers
     source          1..10460
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        165..404
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
     CDS             405..4583
                     /codon_start=1
                     /gene="S. cerevisiae LYS2"
                     /product="alpha-aminoadipate reductase, required for lysine
                     biosynthesis"
                     /label=LYS2
                     /note="yeast auxotrophic marker"
                     /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD
                     VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN
                     ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN
                     LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS
                     LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI
                     VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA
                     KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD
                     HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT
                     MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL
                     LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS
                     KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG
                     LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC
                     CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD
                     DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP
                     NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD
                     SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV
                     VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD
                     LLGRSPKNYSFKVFAHVRAKDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQF
                     GLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDFV
                     SSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMNSASGLTGGYGQSKWAAEYIIRRAGE
                     RGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVARV
                     VVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVID
                     RNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEVG
                     IYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA"
     promoter        4856..5263
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             5264..6358
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     rep_origin      complement(6921..7376)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7058..7079)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7270..7289
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7502..7524
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7516..7533
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7517..7533
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7540..7559
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7540..7558
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    7567..7674
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(7686..7704)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7725..7741)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7725..7741)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7738..7760)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7749..7765
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7773..7779,7780..7797,7798..7803))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7818..7839
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7956..7973)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8127..8715)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8207..8226)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8886..9677,9678..9746))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9509..9528
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9747..9851)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10433..10451
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcggcgc gtcgaggaaa actcttcaat agttttgcca gcggaattcc acttgcaatt
      181 acataaaaaa ttccggcggt ttttcgcgtg tgactcaatg tcgaaatacc tgcctaatga
      241 acatgaacat cgcccaaatg tatttgaaga cccgctggga gaagttcaag atatataagt
      301 aacaagcagc caatagtata aaaaaaaatc tgagtttatt acctttcctg gaatttcagt
      361 gaaaaactgc taattataga gagatatcac agagttactc actaatgact aacgaaaagg
      421 tctggataga gaagttggat aatccaactc tttcagtgtt accacatgac tttttacgcc
      481 cacaacaaga accttatacg aaacaagcta catattcgtt acagctacct cagctcgatg
      541 tgcctcatga tagtttttct aacaaatacg ctgtcgcttt gagtgtatgg gctgcattga
      601 tatatagagt aaccggtgac gatgatattg ttctttatat tgcgaataac aaaatcttaa
      661 gattcaatat tcaaccaacg tggtcattta atgagctgta ttctacaatt aacaatgagt
      721 tgaacaagct caattctatt gaggccaatt tttcctttga cgagctagct gaaaaaattc
      781 aaagttgcca agatctggaa aggacccctc agttgttccg tttggccttt ttggaaaacc
      841 aagatttcaa attagacgag ttcaagcatc atttagtgga ctttgctttg aatttggata
      901 ccagtaataa tgcgcatgtt ttgaacttaa tttataacag cttactgtat tcgaatgaaa
      961 gagtaaccat tgttgcggac caatttactc aatatttgac tgctgcgcta agcgatccat
     1021 ccaattgcat aactaaaatc tctctgatca ccgcatcatc caaggatagt ttacctgatc
     1081 caactaagaa cttgggctgg tgcgatttcg tggggtgtat tcacgacatt ttccaggaca
     1141 atgctgaagc cttcccagag agaacctgtg ttgtggagac tccaacacta aattccgaca
     1201 agtcccgttc tttcacttat cgcgacatca accgcacttc taacatagtt gcccattatt
     1261 tgattaaaac aggtatcaaa agaggtgatg tagtgatgat ctattcttct aggggtgtgg
     1321 atttgatggt atgtgtgatg ggtgtcttga aagccggcgc aaccttttca gttatcgacc
     1381 ctgcatatcc cccagccaga caaaccattt acttaggtgt tgctaaacca cgtgggttga
     1441 ttgttattag agctgctgga caattggatc aactagtaga agattacatc aatgatgaat
     1501 tggagattgt ttcaagaatc aattccatcg ctattcaaga aaatggtacc attgaaggtg
     1561 gcaaattgga caatggcgag gatgttttgg ctccatatga tcactacaaa gacaccagaa
     1621 caggtgttgt agttggacca gattccaacc caaccctatc tttcacatct ggttccgaag
     1681 gtattcctaa gggtgttctt ggtagacatt tttccttggc ttattatttc aattggatgt
     1741 ccaaaaggtt caacttaaca gaaaatgata aattcacaat gctgagcggt attgcacatg
     1801 atccaattca aagagatatg tttacaccat tatttttagg tgcccaattg tatgtcccta
     1861 ctcaagatga tattggtaca ccgggccgtt tagcggaatg gatgagtaag tatggttgca
     1921 cagttaccca tttaacacct gccatgggtc aattacttac tgcccaagct actacaccat
     1981 tccctaagtt acatcatgcg ttctttgtgg gtgacatttt aacaaaacgt gattgtctga
     2041 ggttacaaac cttggcagaa aattgccgta ttgttaatat gtacggtacc actgaaacac
     2101 agcgtgcagt ttcttatttc gaagttaaat caaaaaatga cgatccaaac tttttgaaaa
     2161 aattgaaaga tgtcatgcct gctggtaaag gtatgttgaa cgttcagcta ctagttgtta
     2221 acaggaacga tcgtactcaa atatgtggta ttggcgaaat aggtgagatt tatgttcgtg
     2281 caggtggttt ggccgaaggt tatagaggat taccagaatt gaataaagaa aaatttgtga
     2341 acaactggtt tgttgaaaaa gatcactgga attatttgga taaggataat ggtgaacctt
     2401 ggagacaatt ctggttaggt ccaagagata gattgtacag aacgggtgat ttaggtcgtt
     2461 atctaccaaa cggtgactgt gaatgttgcg gtagggctga tgatcaagtt aaaattcgtg
     2521 ggttcagaat cgaattagga gaaatagata cgcacatttc ccaacatcca ttggtaagag
     2581 aaaacattac tttagttcgc aaaaatgccg acaatgagcc aacattgatc acatttatgg
     2641 tcccaagatt tgacaagcca gatgacttgt ctaagttcca aagtgatgtt ccaaaggagg
     2701 ttgaaactga ccctatagtt aagggcttaa tcggttacca tcttttatcc aaggacatca
     2761 ggactttctt aaagaaaaga ttggctagct atgctatgcc ttccttgatt gtggttatgg
     2821 ataaactacc attgaatcca aatggtaaag ttgataagcc taaacttcaa ttcccaactc
     2881 ccaagcaatt aaatttggta gctgaaaata cagtttctga aactgacgac tctcagttta
     2941 ccaatgttga gcgcgaggtt agagacttat ggttaagtat attacctacc aagccagcat
     3001 ctgtatcacc agatgattcg tttttcgatt taggtggtca ttctatcttg gctaccaaaa
     3061 tgatttttac cttaaagaaa aagctgcaag ttgatttacc attgggcaca attttcaagt
     3121 atccaacgat aaaggccttt gccgcggaaa ttgacagaat taaatcatcg ggtggatcat
     3181 ctcaaggtga ggtcgtcgaa aatgtcactg caaattatgc ggaagacgcc aagaaattgg
     3241 ttgagacgct accaagttcg tacccctctc gagaatattt tgttgaacct aatagtgccg
     3301 aaggaaaaac aacaattaat gtgtttgtta ccggtgtcac aggatttctg ggctcctaca
     3361 tccttgcaga tttgttagga cgttctccaa agaactacag tttcaaagtg tttgcccacg
     3421 tcagggccaa ggatgaagaa gctgcatttg caagattaca aaaggcaggt atcacctatg
     3481 gtacttggaa cgaaaaattt gcctcaaata ttaaagttgt attaggcgat ttatctaaaa
     3541 gccaatttgg tctttcagat gagaagtgga tggatttggc aaacacagtt gatataatta
     3601 tccataatgg tgcgttagtt cactgggttt atccatatgc caaattgagg gatccaaatg
     3661 ttatttcaac tatcaatgtt atgagcttag ccgccgtcgg caagccaaag ttctttgact
     3721 ttgtttcctc cacttctact cttgacactg aatactactt taatttgtca gataaacttg
     3781 ttagcgaagg gaagccaggc attttagaat cagacgattt aatgaactct gcaagcgggc
     3841 tcactggtgg atatggtcag tccaaatggg ctgctgagta catcattaga cgtgcaggtg
     3901 aaaggggcct acgtgggtgt attgtcagac caggttacgt aacaggtgcc tctgccaatg
     3961 gttcttcaaa cacagatgat ttcttattga gatttttgaa aggttcagtc caattaggta
     4021 agattccaga tatcgaaaat tccgtgaata tggttccagt agatcatgtt gctcgtgttg
     4081 ttgttgctac gtctttgaat cctcccaaag aaaatgaatt ggccgttgct caagtaacgg
     4141 gtcacccaag aatattattc aaagactact tgtatacttt acacgattat ggttacgatg
     4201 tcgaaatcga aagctattct aaatggaaga aatcattgga ggcgtctgtt attgacagga
     4261 atgaagaaaa tgcgttgtat cctttgctac acatggtctt agacaactta cctgaaagta
     4321 ccaaagctcc ggaactagac gataggaacg ccgtggcatc tttaaagaaa gacaccgcat
     4381 ggacaggtgt tgattggtct aatggaatag gtgttactcc agaagaggtt ggtatatata
     4441 ttgcattttt aaacaaggtt ggatttttac ctccaccaac tcataatgac aaacttccac
     4501 tgccaagtat agaactaact caagcgcaaa taagtctagt tgcttcaggt gctggtgctc
     4561 gtggaagctc cgcagcagct taaggttgag cattacgtat gatatgtgcg cgccatcgag
     4621 gagaacttct agtatatcta catacctaat attattgcct tattaaaaat ggaatcccaa
     4681 caattacatc aaaatccaca ttctcttcaa aatcaattgt cctgtacttc cttgttcatg
     4741 tgtgttcaaa aacgttatat ttataggata attatactct atttctcaac aagtaattgg
     4801 ttgtttggcc gagcggtcta aggcgcctga ttcaagaaat atcttgaccg cagttaactg
     4861 tgggaatact caggtatcgt aagatgcaag agttcgaatc tcttagcaac cattattttt
     4921 ttcctcaaca taacgagaac acacaggggc gctatcgcac agaatcaaat tcgatgactg
     4981 gaaatttttt gttaatttca gaggtcgcct gacgcatata cctttttcaa ctgaaaaatt
     5041 gggagaaaaa ggaaaggtga gagcgccgga accggctttt catatagaat agagaagcgt
     5101 tcatgactaa atgcttgcat cacaatactt gaagttgaca atattattta aggacctatt
     5161 gttttttcca ataggtggtt agcaatcgtc ttactttcta acttttctta ccttttacat
     5221 ttcagcaata tatatatata tatttcaagg atataccatt ctaatgtctg cccctaagaa
     5281 gatcgtcgtt ttgccaggtg accacgttgg tcaagaaatc acagccgaag ccattaaggt
     5341 tcttaaagct atttctgatg ttcgttccaa tgtcaagttc gatttcgaaa atcatttaat
     5401 tggtggtgct gctatcgatg ctacaggtgt tccacttcca gatgaggcgc tggaagcctc
     5461 caagaaggct gatgccgttt tgttaggtgc tgtgggtggt cctaaatggg gtaccggtag
     5521 tgttagacct gaacaaggtt tactaaaaat ccgtaaagaa cttcaattgt acgccaactt
     5581 aagaccatgt aactttgcat ccgactctct tttagactta tctccaatca agccacaatt
     5641 tgctaaaggt actgacttcg ttgttgtcag agaattagtg ggaggtattt actttggtaa
     5701 gagaaaggaa gacgatggtg atggtgtcgc ttgggatagt gaacaataca ccgttccaga
     5761 agtgcaaaga atcacaagaa tggccgcttt catggcccta caacatgagc caccattgcc
     5821 tatttggtcc ttggataaag ctaatgtttt ggcctcttca agattatgga gaaaaactgt
     5881 ggaggaaacc atcaagaacg aattccctac attgaaggtt caacatcaat tgattgattc
     5941 tgccgccatg atcctagtta agaacccaac ccacctaaat ggtattataa tcaccagcaa
     6001 catgtttggt gatatcatct ccgatgaagc ctccgttatc ccaggttcct tgggtttgtt
     6061 gccatctgcg tccttggcct ctttgccaga caagaacacc gcatttggtt tgtacgaacc
     6121 atgccacggt tctgctccag atttgccaaa gaataaggtc aaccctatcg ccactatctt
     6181 gtctgctgca atgatgttga aattgtcatt gaacttgcct gaagaaggta aggccattga
     6241 agatgcagtt aaaaaggttt tggatgcagg tatcagaact ggtgatttag gtggttccaa
     6301 cagtaccacc gaagtcggtg atgctgtcgc cgaagaagtt aagaaaatcc ttgcttaaaa
     6361 agattctctt tttttatgat atttgtacat aaactttata aatgaaattc ataatagaaa
     6421 cgacacgaaa ttacaaaatg gaatatgttc atagggtaga cgaaactata tacgcaatct
     6481 acatacattt atcaagaagg agaaaaagga ggatgtaaag gaatacaggt aagcaaattg
     6541 atactaatgg ctcaacgtga taaggaaaaa gaattgcact ttaacattaa tattgacaag
     6601 gaggagggca ccacacaaaa agttaggtgt aacagaaaat catgaaacta tgattcctaa
     6661 tttatatatt ggaggatttt ctctaaaaaa aaaaaaatac aacaaataaa aaacactcaa
     6721 tgacctgacc atttgatgga gtttaagtca ataccttctt gaaccatttc ccataatggt
     6781 gaaagttccc tcaagaattt tactctgtca gaaacggcct tacgacgtag tcgaggatct
     6841 cctaggttaa ttaagtttaa acagatccta tgcggtgtga aataccgcac agatgcgtaa
     6901 ggagaaaata ccgcatcagg aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     6961 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     7021 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     7081 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     7141 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     7201 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     7261 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     7321 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat
     7381 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     7441 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     7501 tcccagtcac gacgttgtaa aacgacggcc agtgaattgt aatacgactc actatagggc
     7561 gaattggagc tccaccgcgg tggcggccgc tctagaacta gtgggtcccc cgggctgcag
     7621 gaattcgata tcaagcttat cgataccgtc gacctggagg gggggcccgg taccagcttt
     7681 tgttcccttt agtgagggtt aatttcgagc ttggcgtaat catggtcata gctgtttcct
     7741 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     7801 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     7861 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     7921 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     7981 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     8041 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     8101 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     8161 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     8221 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     8281 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     8341 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     8401 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     8461 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     8521 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     8581 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     8641 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     8701 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     8761 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     8821 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     8881 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     8941 tccatagttg cctgactgcc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     9001 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     9061 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     9121 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     9181 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     9241 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgaaaa
     9301 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     9361 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     9421 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     9481 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     9541 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     9601 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     9661 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     9721 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     9781 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     9841 ggggttccgc gcacatttcc ccgaaaagtg ccacctatcg cttgcctgta acttacacgc
     9901 gcctcgtatc ttttaatgat ggaataattt gggaatttac tctgtgttta tttattttta
     9961 tgttttgtat ttggatttta gaaagtaaat aaagaaggta gaagagttac ggaatgaaga
    10021 aaaaaaaata aacaaaggtt taaaaaattt caacaaaaag cgtactttac atatatattt
    10081 attagacaag aaaagcagat taaatagata tacattcgat taacgataag taaaatgtaa
    10141 aatcacagga ttttcgtgtg tggtcttcta cacagacaag atgaaacaat tcggcattaa
    10201 tacctgagag caggaagagc aagataaaag gtagtatttg ttggcgatcc ccctagagtc
    10261 ttttacatct tcggaaaaca aaaactattt tttctttaat ttcttttttt actttctatt
    10321 tttaatttat atatttatat taaaaaattt aaattataat tatttttata gcacgtgatg
    10381 aaaaggaccc ccgtcctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
    10441 tatcacgagg ccctttcgtc
//
