LOCUS       sequence-107504-        9242 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  harbours marker genes URA3 and LYS2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9242)
  AUTHORS   Mulleder M, Campbell K, Matsarskaia O, Eckerstorfer F, Ralser M
  TITLE     Saccharomyces cerevisiae single-copy plasmids for auxotrophy 
            compensation, multiple marker selection, and for designing 
            metabolically cooperating communities.
  JOURNAL   F1000Res. 2016 Sep 20;5:2351. eCollection 2016.
  PUBMED    27830062
REFERENCE   2  (bases 1 to 9242)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "F1000Res.";
            date: "2016-09-20"; volume: "5"; pages: "2351. eCollection 2016"
COMMENT     Sequence Label: pUK
FEATURES             Location/Qualifiers
     source          1..9242
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        164..403
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
     CDS             404..4582
                     /codon_start=1
                     /gene="S. cerevisiae LYS2"
                     /product="alpha-aminoadipate reductase, required for lysine
                     biosynthesis"
                     /label=LYS2
                     /note="yeast auxotrophic marker"
                     /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD
                     VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN
                     ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN
                     LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS
                     LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI
                     VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA
                     KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD
                     HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT
                     MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL
                     LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS
                     KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG
                     LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC
                     CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD
                     DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP
                     NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD
                     SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV
                     VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD
                     LLGRSPKNYSFKVFAHVRAKDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQF
                     GLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDFV
                     SSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMNSASGLTGGYGQSKWAAEYIIRRAGE
                     RGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVARV
                     VVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVID
                     RNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEVG
                     IYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA"
     CDS             4769..5572
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(5703..6158)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5840..5861)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6052..6071
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6284..6306
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6298..6315
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6299..6315
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6322..6341
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6322..6340
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    6349..6456
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(6468..6486)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6507..6523)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6507..6523)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6520..6542)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6531..6547
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6555..6561,6562..6579,6580..6585))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6600..6621
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6738..6755)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6909..7497)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6989..7008)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7668..8459,8460..8528))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8291..8310
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8529..8633)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9215..9233
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggcg tcgaggaaaa ctcttcaata gttttgccag cggaattcca cttgcaatta
      181 cataaaaaat tccggcggtt tttcgcgtgt gactcaatgt cgaaatacct gcctaatgaa
      241 catgaacatc gcccaaatgt atttgaagac ccgctgggag aagttcaaga tatataagta
      301 acaagcagcc aatagtataa aaaaaaatct gagtttatta cctttcctgg aatttcagtg
      361 aaaaactgct aattatagag agatatcaca gagttactca ctaatgacta acgaaaaggt
      421 ctggatagag aagttggata atccaactct ttcagtgtta ccacatgact ttttacgccc
      481 acaacaagaa ccttatacga aacaagctac atattcgtta cagctacctc agctcgatgt
      541 gcctcatgat agtttttcta acaaatacgc tgtcgctttg agtgtatggg ctgcattgat
      601 atatagagta accggtgacg atgatattgt tctttatatt gcgaataaca aaatcttaag
      661 attcaatatt caaccaacgt ggtcatttaa tgagctgtat tctacaatta acaatgagtt
      721 gaacaagctc aattctattg aggccaattt ttcctttgac gagctagctg aaaaaattca
      781 aagttgccaa gatctggaaa ggacccctca gttgttccgt ttggcctttt tggaaaacca
      841 agatttcaaa ttagacgagt tcaagcatca tttagtggac tttgctttga atttggatac
      901 cagtaataat gcgcatgttt tgaacttaat ttataacagc ttactgtatt cgaatgaaag
      961 agtaaccatt gttgcggacc aatttactca atatttgact gctgcgctaa gcgatccatc
     1021 caattgcata actaaaatct ctctgatcac cgcatcatcc aaggatagtt tacctgatcc
     1081 aactaagaac ttgggctggt gcgatttcgt ggggtgtatt cacgacattt tccaggacaa
     1141 tgctgaagcc ttcccagaga gaacctgtgt tgtggagact ccaacactaa attccgacaa
     1201 gtcccgttct ttcacttatc gcgacatcaa ccgcacttct aacatagttg cccattattt
     1261 gattaaaaca ggtatcaaaa gaggtgatgt agtgatgatc tattcttcta ggggtgtgga
     1321 tttgatggta tgtgtgatgg gtgtcttgaa agccggcgca accttttcag ttatcgaccc
     1381 tgcatatccc ccagccagac aaaccattta cttaggtgtt gctaaaccac gtgggttgat
     1441 tgttattaga gctgctggac aattggatca actagtagaa gattacatca atgatgaatt
     1501 ggagattgtt tcaagaatca attccatcgc tattcaagaa aatggtacca ttgaaggtgg
     1561 caaattggac aatggcgagg atgttttggc tccatatgat cactacaaag acaccagaac
     1621 aggtgttgta gttggaccag attccaaccc aaccctatct ttcacatctg gttccgaagg
     1681 tattcctaag ggtgttcttg gtagacattt ttccttggct tattatttca attggatgtc
     1741 caaaaggttc aacttaacag aaaatgataa attcacaatg ctgagcggta ttgcacatga
     1801 tccaattcaa agagatatgt ttacaccatt atttttaggt gcccaattgt atgtccctac
     1861 tcaagatgat attggtacac cgggccgttt agcggaatgg atgagtaagt atggttgcac
     1921 agttacccat ttaacacctg ccatgggtca attacttact gcccaagcta ctacaccatt
     1981 ccctaagtta catcatgcgt tctttgtggg tgacatttta acaaaacgtg attgtctgag
     2041 gttacaaacc ttggcagaaa attgccgtat tgttaatatg tacggtacca ctgaaacaca
     2101 gcgtgcagtt tcttatttcg aagttaaatc aaaaaatgac gatccaaact ttttgaaaaa
     2161 attgaaagat gtcatgcctg ctggtaaagg tatgttgaac gttcagctac tagttgttaa
     2221 caggaacgat cgtactcaaa tatgtggtat tggcgaaata ggtgagattt atgttcgtgc
     2281 aggtggtttg gccgaaggtt atagaggatt accagaattg aataaagaaa aatttgtgaa
     2341 caactggttt gttgaaaaag atcactggaa ttatttggat aaggataatg gtgaaccttg
     2401 gagacaattc tggttaggtc caagagatag attgtacaga acgggtgatt taggtcgtta
     2461 tctaccaaac ggtgactgtg aatgttgcgg tagggctgat gatcaagtta aaattcgtgg
     2521 gttcagaatc gaattaggag aaatagatac gcacatttcc caacatccat tggtaagaga
     2581 aaacattact ttagttcgca aaaatgccga caatgagcca acattgatca catttatggt
     2641 cccaagattt gacaagccag atgacttgtc taagttccaa agtgatgttc caaaggaggt
     2701 tgaaactgac cctatagtta agggcttaat cggttaccat cttttatcca aggacatcag
     2761 gactttctta aagaaaagat tggctagcta tgctatgcct tccttgattg tggttatgga
     2821 taaactacca ttgaatccaa atggtaaagt tgataagcct aaacttcaat tcccaactcc
     2881 caagcaatta aatttggtag ctgaaaatac agtttctgaa actgacgact ctcagtttac
     2941 caatgttgag cgcgaggtta gagacttatg gttaagtata ttacctacca agccagcatc
     3001 tgtatcacca gatgattcgt ttttcgattt aggtggtcat tctatcttgg ctaccaaaat
     3061 gatttttacc ttaaagaaaa agctgcaagt tgatttacca ttgggcacaa ttttcaagta
     3121 tccaacgata aaggcctttg ccgcggaaat tgacagaatt aaatcatcgg gtggatcatc
     3181 tcaaggtgag gtcgtcgaaa atgtcactgc aaattatgcg gaagacgcca agaaattggt
     3241 tgagacgcta ccaagttcgt acccctctcg agaatatttt gttgaaccta atagtgccga
     3301 aggaaaaaca acaattaatg tgtttgttac cggtgtcaca ggatttctgg gctcctacat
     3361 ccttgcagat ttgttaggac gttctccaaa gaactacagt ttcaaagtgt ttgcccacgt
     3421 cagggccaag gatgaagaag ctgcatttgc aagattacaa aaggcaggta tcacctatgg
     3481 tacttggaac gaaaaatttg cctcaaatat taaagttgta ttaggcgatt tatctaaaag
     3541 ccaatttggt ctttcagatg agaagtggat ggatttggca aacacagttg atataattat
     3601 ccataatggt gcgttagttc actgggttta tccatatgcc aaattgaggg atccaaatgt
     3661 tatttcaact atcaatgtta tgagcttagc cgccgtcggc aagccaaagt tctttgactt
     3721 tgtttcctcc acttctactc ttgacactga atactacttt aatttgtcag ataaacttgt
     3781 tagcgaaggg aagccaggca ttttagaatc agacgattta atgaactctg caagcgggct
     3841 cactggtgga tatggtcagt ccaaatgggc tgctgagtac atcattagac gtgcaggtga
     3901 aaggggccta cgtgggtgta ttgtcagacc aggttacgta acaggtgcct ctgccaatgg
     3961 ttcttcaaac acagatgatt tcttattgag atttttgaaa ggttcagtcc aattaggtaa
     4021 gattccagat atcgaaaatt ccgtgaatat ggttccagta gatcatgttg ctcgtgttgt
     4081 tgttgctacg tctttgaatc ctcccaaaga aaatgaattg gccgttgctc aagtaacggg
     4141 tcacccaaga atattattca aagactactt gtatacttta cacgattatg gttacgatgt
     4201 cgaaatcgaa agctattcta aatggaagaa atcattggag gcgtctgtta ttgacaggaa
     4261 tgaagaaaat gcgttgtatc ctttgctaca catggtctta gacaacttac ctgaaagtac
     4321 caaagctccg gaactagacg ataggaacgc cgtggcatct ttaaagaaag acaccgcatg
     4381 gacaggtgtt gattggtcta atggaatagg tgttactcca gaagaggttg gtatatatat
     4441 tgcattttta aacaaggttg gatttttacc tccaccaact cataatgaca aacttccact
     4501 gccaagtata gaactaactc aagcgcaaat aagtctagtt gcttcaggtg ctggtgctcg
     4561 tggaagctcc gcagcagctt aaggttgagc attacgtatg atatgtgcat gcgattcggt
     4621 aatctccgaa cagaaggaag aacgaaggaa ggagcacaga cttagattgg tatatatacg
     4681 catatgtagt gttgaagaaa catgaaattg cccagtattc ttaacccaac tgcacagaac
     4741 aaaaacctgc aggaaacgaa gataaatcat gtcgaaagct acatataagg aacgtgctgc
     4801 tactcatcct agtcctgttg ctgccaagct atttaatatc atgcacgaaa agcaaacaaa
     4861 cttgtgtgct tcattggatg ttcgtaccac caaggaatta ctggagttag ttgaagcatt
     4921 aggtcccaaa atttgtttac taaaaacaca tgtggatatc ttgactgatt tttccatgga
     4981 gggcacagtt aagccgctaa aggcattatc cgccaagtac aattttttac tcttcgaaga
     5041 cagaaaattt gctgacattg gtaatacagt caaattgcag tactctgcgg gtgtatacag
     5101 aatagcagaa tgggcagaca ttacgaatgc acacggtgtg gtgggcccag gtattgttag
     5161 cggtttgaag caggcggcag aagaagtaac aaaggaacct agaggccttt tgatgttagc
     5221 agaattgtca tgcaagggct ccctatctac tggagaatat actaagggta ctgttgacat
     5281 tgcgaagagc gacaaagatt ttgttatcgg ctttattgct caaagagaca tgggtggaag
     5341 agatgaaggt tacgattggt tgattatgac acccggtgtg ggtttagatg acaagggaga
     5401 cgcattgggt caacagtata gaaccgtgga tgatgtggtc tctacaggat ctgacattat
     5461 tattgttgga agaggactat ttgcaaaggg aagggatgct aaggtagagg gtgaacgtta
     5521 cagaaaagca ggctgggaag catatttgag aagatgcggc cagcaaaact aaaaaactgt
     5581 attataagta aatgcatgta tactaaactc acaaattaga gcttcaattt aattatatca
     5641 gttattaccc tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     5701 ggaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     5761 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     5821 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     5881 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     5941 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     6001 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     6061 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     6121 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc attcgccatt caggctgcgc
     6181 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
     6241 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
     6301 aaaacgacgg ccagtgaatt gtaatacgac tcactatagg gcgaattgga gctccaccgc
     6361 ggtggcggcc gctctagaac tagtgggtcc cccgggctgc aggaattcga tatcaagctt
     6421 atcgataccg tcgacctgga gggggggccc ggtaccagct tttgttccct ttagtgaggg
     6481 ttaatttcga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     6541 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     6601 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     6661 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     6721 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     6781 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     6841 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     6901 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     6961 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     7021 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     7081 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     7141 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     7201 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     7261 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     7321 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     7381 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     7441 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     7501 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     7561 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     7621 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     7681 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactg
     7741 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     7801 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     7861 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     7921 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     7981 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     8041 caacgatcaa ggcgagttac atgatccccc atgttgtgaa aaaaagcggt tagctccttc
     8101 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     8161 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     8221 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     8281 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     8341 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     8401 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     8461 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     8521 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     8581 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     8641 ccccgaaaag tgccacctat cgcttgcctg taacttacac gcgcctcgta tcttttaatg
     8701 atggaataat ttgggaattt actctgtgtt tatttatttt tatgttttgt atttggattt
     8761 tagaaagtaa ataaagaagg tagaagagtt acggaatgaa gaaaaaaaaa taaacaaagg
     8821 tttaaaaaat ttcaacaaaa agcgtacttt acatatatat ttattagaca agaaaagcag
     8881 attaaataga tatacattcg attaacgata agtaaaatgt aaaatcacag gattttcgtg
     8941 tgtggtcttc tacacagaca agatgaaaca attcggcatt aatacctgag agcaggaaga
     9001 gcaagataaa aggtagtatt tgttggcgat ccccctagag tcttttacat cttcggaaaa
     9061 caaaaactat tttttcttta atttcttttt ttactttcta tttttaattt atatatttat
     9121 attaaaaaat ttaaattata attattttta tagcacgtga tgaaaaggac ccccgtccta
     9181 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     9241 tc
//
