LOCUS       sequence-107510-         755 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tetracycline biosensor. Low copy plasmid that expresses GFP 
            proportional to tetracycline concentration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Rogers JK, Guzman CD, Taylor ND, Raman S, Anderson K, Church GM
  TITLE     Synthetic biosensors for precise gene control and real-time 
            monitoring of metabolites.
  JOURNAL   Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
  PUBMED    26152303
REFERENCE   2  (bases 1 to 755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Jul 7. pii: gkv616."
COMMENT     Sequence Label: pJKR-L-tetR
FEATURES             Location/Qualifiers
     source          1..755
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     280..299
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     RBS             380..388
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 accttgttgg cataaaaagg ctaattgatt ttcgagagtt tcatactgtt tttctgtagg
       61 ccgtgtacct aaatgtactt ttgctccatc gcgatgactt agtaaagcac atctaaaact
      121 tttagcgtta ttacgtaaaa aatcttgcca gctttcccct tctaaagggc aaaagtgagt
      181 atggtgccta tctaacatct caatggctaa ggcgtcgagc aaagcccgct tattttttac
      241 atgccaatac aatgtaggct gctctacacc tagcttctgg gcgagtttac gggttgttaa
      301 accttcgatt ccgacctcat taagcagctc taatgcgctg ttaatcactt tacttttatc
      361 taaacgagac atttgtatta cctccttatt tcaaagttaa acaaaattat ttgtagaggg
      421 aaaccgttgt ggtctccctg aatatagcct acgagcctta tgcatgcccg taaagttatc
      481 cagcaaccac tcatagacct agggcagcag atagggacga cgtggtgtta gctgtggtaa
      541 acgccatggg cattaaaaaa tgccctcttg ggttatcaag agggtcatta tatttgctga
      601 ggagtgccgt taattaaggc gcaacgcaat taatgtaagt tagctcactc attagggtta
      661 tgctagaact ggcatgcatc tttgtacagt tcgtccatac cgtgggtgat accagcagcg
      721 gtaacgaatt ccagcagaac catgtggtca cgttt
//
