LOCUS       sequence-107511-         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tetracycline biosensor. High copy plasmid that expresses GFP 
            proportional to tetracycline concentration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Rogers JK, Guzman CD, Taylor ND, Raman S, Anderson K, Church GM
  TITLE     Synthetic biosensors for precise gene control and real-time 
            monitoring of metabolites.
  JOURNAL   Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
  PUBMED    26152303
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Jul 7. pii: gkv616."
COMMENT     Sequence Label: pJKR-H-tetR
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             37..45
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 atcactttac ttttatctaa acgagacatt tgtattacct ccttatttca aagttaaaca
       61 aaattatttg tagagggaaa ccgttgtggt ctccctgaat atagcctacg agccttatgc
      121 atgcccgtaa agttatccag caaccactca tagacctagg gcagcagata gggacgacgt
      181 ggtgttagct gtggtaaacg ccatgggcat taaaaaatgc cctcttgggt tatcaagagg
      241 gtcattatat ttgctgagga gtgccgttaa ttaaggcgca acgcaattaa tgtaagttag
      301 ctcactcatt agggttatgc tagaactggc atgcatcttt gtacagttcg tccataccgt
      361 gggtgatacc agcagcggta acgaattcca gcagaaccat gtggtcacgt ttttcgttcg
      421 ggtctttaga cagaacagac tgggtagaca ggtagtggtt gtccggcaac agaaccggac
      481 cgtcaccgat cggggtgttc tgctggtagt ggtcagccag ctgaacagaa ccgtcttcaa
      541 cgttgtgacg gattttgaag ttagctttga taccgttttt ctgtttgtca gcggtgatgt
      601 aaacgttgtg agagttgaag ttgtattcca gtttgtgacc caggatgtta ccgtcttctt
      661 tgaagtcgat acctttcagt tcgatacggt taaccagggt gtcaccttcg aatttaactt
      721 cagcacgggt tttgtaggta ccgtcgtctt tgaaagagat ggtacgttcc tgaacgtaac
      781 cttccggcat agcagatttg aagaagtcgt gctgtttcat gtggtccggg taacgagcga
      841 agcactgaac accgtaggtc agggtggtaa ccagggtc
//
