LOCUS       sequence-107514-         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Flavonoid biosensor. High copy plasmid that expresses GFP 
            proportional to flavonoid concentration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Rogers JK, Guzman CD, Taylor ND, Raman S, Anderson K, Church GM
  TITLE     Synthetic biosensors for precise gene control and real-time 
            monitoring of metabolites.
  JOURNAL   Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
  PUBMED    26152303
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Jul 7. pii: gkv616."
COMMENT     Sequence Label: pJKR-H-ttgR
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(81..128)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     RBS             822..830
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 tattattgaa gcatttatca gggttattgt ctcatgagcg gaagtgtgac cgtgtgcttc
       61 tcaaatgcct gaggtttcag caaaaaaccc ctcaagaccc gtttagaggc cccaaggggt
      121 tatgctagtt attgctcgaa ctggcatgtt ggtttcctac attcaatttt ttagtcgctt
      181 attatttgcg cagcgccggg ctcagacgca gcatatccag accggtatca acccattttt
      241 ccacatcgcc cagcaggtca acactatccg gcagcagcag ccaacgacca atcaggccat
      301 ccacataggc aaacatcgca accgctgcgc gttccacatc cagttcaccc ggcagctgac
      361 cgcgacgaac tgcgtttgcc agtgccaggg tgataccttt atgacaatcc agcacggcgc
      421 tctggcgctg ctgacgaatt tcacacatat catccgtaaa ttcgcatttg tgatgcagga
      481 tttcattaat gcgacgggta cgtgcatcca gaaccagttc gttaaacacc tgcagcagca
      541 gtttgcgcat gcagcccagc gggtccagtt catcttcaga ttcgcttgca cgggccaggt
      601 gatcatgcgt ttcgtgcaga gaatccagca gtgcctgaac cagttcggct ttattgttga
      661 aatgccagta gattgcaccg cgggtaacac ctgccagttc tgcaatatct gccagcgtgg
      721 tacgtgccac accacgttta taaaacgcgc gttcggccgc ttcgataatc tgcgcacgcg
      781 tttcctgtgc ttcttctttg gtgcgacgca ccatttgtat tacctcctta tttcaaagtt
      841 aaacaaaatt atttgtagag ggaaaccgtt gtggtctccc tgaatatagc ctacgagcct
      901 tatgcatg
//
