LOCUS       sequence-107516-         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Glucarate biosensor. Low copy plasmid that expresses GFP 
            proportional to glucarate concentration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Rogers JK, Guzman CD, Taylor ND, Raman S, Anderson K, Church GM
  TITLE     Synthetic biosensors for precise gene control and real-time 
            monitoring of metabolites.
  JOURNAL   Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
  PUBMED    26152303
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Jul 7. pii: gkv616."
COMMENT     Sequence Label: pJKR-L-cdaR
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(77..124)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tattattgaa gcatttatca gggttattgt ctcatgagcg gaagtgtgac cgtgtgcttc
       61 tcgcctgagg tttcagcaaa aaacccctca agacccgttt agaggcccca aggggttatg
      121 ctagttattg ctcgaactgg catgttggtt tcctacattc aattttttag tcgcttacta
      181 ccgctcttca tccagttgta acgccacata cagcagcaac ctgtcatcaa aattgcccaa
      241 atcaagcccg gtcagttccg atatacgatt aagccgatac tccagggtat tacgatgaat
      301 aaacaacgcc tttgacgttg ccagcggttg cacattgtgg cgaaaccacg ccgccagcgt
      361 tcgtcgcagc aagccgttat tgtccatcgt tttcagccgc gccagcggtc gcgccagttc
      421 gttggcctgc cagtcgccac gcaaactgtc gagtaacaca ggtaacatca gatcctgata
      481 aaaatagcag cgactttctg gcatccgctg tttacccacc accatcgtcg ttttcgccgt
      541 acgataggat cgggcaatac tgctaggacc ggtaaaatag ttgcccagtg aaacgcgaaa
      601 acgcagctgg ccgtactctt tcatgcgggt aatcagttgt tcaactcgct tacgatgatc
      661 ttctgcatcc cagcgcccaa aagagttcaa cgccggtttc aacaccacca tttcggttag
      721 cgagacaatc gccaccagat tattacgctc gggcgtagtc agcgcgtttt gcagttgttg
      781 taactccgcc attgcgctgt ccacgccaag ctgaccgctg tcgacctcaa caatagccac
      841 cactcgcgg
//
