LOCUS       sequence-107519-         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice 
            ubiquitin promoter for Agrobacterium-mediated transformation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Xie K, Minkenberg B, Yang Y
  TITLE     Boosting CRISPR/Cas9 multiplex editing capability with the 
            endogenous tRNA-processing system.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Mar 17;112(11):3570-5. doi: 
            10.1073/pnas.1420294112. Epub 2015 Mar 2.
  PUBMED    25733849
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1420294112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-03-17- 17"; volume: "112"; issue: "11"; pages: "3570-5"
COMMENT     Sequence Label: pRGEB32
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        396..471
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 ggaatcttta aacatacgaa cagatcactt aaagttcttc tgaagcaact taaagttatc
       61 aggcatgcat ggatcttgga ggaatcagat gtgcagtcag ggaccatagc acaagacagg
      121 cgtcttctac tggtgctacc agcaaatgct ggaagccggg aacactgggt acgttggaaa
      181 ccacgtgatg tgaagaagta agataaactg taggagaaaa gcatttcgta gtgggccatg
      241 aagcctttca ggacatgtat tgcagtatgg gccggcccat tacgcaattg gacgacaaca
      301 aagactagta ttagtaccac ctcggctatc cacatagatc aaagctgatt taaaagagtt
      361 gtgcagatga tccgtggcag gagaccgagg tctcggtttt agagctagaa atagcaagtt
      421 aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg cttttttgtt
      481 ttagagctag aaatagcaag ttaaaataag gctagtccgt ttttagcgcg tgcatgcctg
      541 caggtccaca aattcgggtc aaggcggaag ccagcgcgcc accccacgtc agcaaatacg
      601 gaggcgcggg gttgacggcg tcacccggtc ctaacggcga ccaacaaacc agccagaaga
      661 aattacagta aaaaaaaagt aaattgcact ttgatccacc ttttattacc taagtctcaa
      721 tttggatcac ccttaaacct atcttttcaa tttgggccgg gttgtggttt ggactaccat
      781 gaacaacttt tcgtcatgtc taacttccct ttcagcaaac atatgaacca tatatagagg
      841 agatcggccg tatactagag ctgatgtgtt taaggtcgtt gattgc
//
