LOCUS       sequence-107529-         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice 
            ubiquitin promoter for Agrobacterium-mediated transformation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Xie K, Minkenberg B, Yang Y
  TITLE     Boosting CRISPR/Cas9 multiplex editing capability with the 
            endogenous tRNA-processing system.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Mar 17;112(11):3570-5. doi: 
            10.1073/pnas.1420294112. Epub 2015 Mar 2.
  PUBMED    25733849
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1420294112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-03-17- 17"; volume: "112"; issue: "11"; pages: "3570-5"
COMMENT     Sequence Label: pRGEB32
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(310..330)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(337..402)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(447..471)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gctgtcgtcc accttggcca tctcgttgct gaagatctct tgcagatagc agatccggtt
       61 cttccgtctg gtgtatcttc ttctggcggt tctcttcagc cgggtggcct cggctgtttc
      121 gccgctgtcg aacagcaggg ctccgatcag gttcttcttg atgctgtgcc ggtcggtgtt
      181 gcccagcacc ttgaatttct tgctgggcac cttgtactcg tcggtgatca cggcccagcc
      241 cacagagttg gtgccgatgt ccaggccgat gctgtacttc ttgtcggctg ctgggactcc
      301 gtggataccg accttccgct tcttctttgg ggccatctta tcgtcatcgt ctttgtaatc
      361 aatatcatga tccttgtagt ctccgtcgtg gtccttatag tccatggtta agtatttcct
      421 tagagaattg gttctatctc cttcgaagcc tgcttttttg tacaaacttg ttgataacta
      481 tctgcaagaa ataatcacca aacagatagg gtaagaacta gcacaagatt aaggacacaa
      541 caacggatca aacagcaaac atattccaac agaacataca tgtattcaaa aagatagtaa
      601 caggcacatc acacatgtat tcagataggt agtaacagca cacgtatcct gaacttcaga
      661 aataagtaga atgaactagt ggcaataatc ttcacacaga tagtaaaaag aaattaccac
      721 aatagattat atagatgaac tacagattgt aacatgatat tactacagat tgtaacatga
      781 tattctaata tagttctta
//
