LOCUS       sequence-107534-         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Glucarate biosensor. High copy plasmid that expresses GFP 
            proportional to glucarate concentration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Rogers JK, Guzman CD, Taylor ND, Raman S, Anderson K, Church GM
  TITLE     Synthetic biosensors for precise gene control and real-time 
            monitoring of metabolites.
  JOURNAL   Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
  PUBMED    26152303
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Jul 7. pii: gkv616."
COMMENT     Sequence Label: pJKR-H-cdaR
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(78..125)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 atattattga agcatttatc agggttattg tctcatgagc ggaagtgtga ccgtgtgctt
       61 ctcgcctgag gtttcagcaa aaaacccctc aagacccgtt tagaggcccc aaggggttat
      121 gctagttatt gctcgaactg gcatgttggt ttcctacatt caatttttta gtcgcttact
      181 accgctcttc atccagttgt aacgccacat acagcagcaa cctgtcatca aaattgccca
      241 aatcaagccc ggtcagttcc gatatacgat taagccgata ctccagggta ttacgatgaa
      301 taaacaacgc ctttgacgtt gccagcggtt gcacattgtg gcgaaaccac gccgccagcg
      361 ttcgtcgcag caagccgtta ttgtccatcg ttttcagccg cgccagcggt cgcgccagtt
      421 cgttggcctg ccagtcgcca cgcaaactgt cgagtaacac aggtaacatc agatcctgat
      481 aaaaatagca gcgactttct ggcatccgct gtttacccac caccatcgtc gttttcgccg
      541 tacgatagga tcgggcaata ctgctaggac cggtaaaata gttgcccagt gaaacgcgaa
      601 aacgcagctg gccgtactct ttcatgcggg taatcagttg ttcaactcgc ttacgatgat
      661 cttctgcatc ccagcgccca aaagagttca acgccggttt caacaccacc atttcggtta
      721 gcgagacaat cgccaccaga ttattacgct cgggcgtagt cagcgcgttt tgcagttgtt
      781 gtaactccgc cattgcgctg tccacgccaa gctgaccgct gtcgacctca acaatagcca
      841 ccactcgcgg ttgattgaga tcga
//
