LOCUS       sequence-107540-         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector for conventional cloning that contains AtU6-26 promoter and 
            sgRNA backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Fauser F, Schiml S, Puchta H
  TITLE     Both CRISPR/Cas-based nucleases and nickases can be used efficiently
            for genome engineering in Arabidopsis thaliana.
  JOURNAL   Plant J. 2014 Jul;79(2):348-59. doi: 10.1111/tpj.12554. Epub 2014 
            Jun 17.
  PUBMED    24836556
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12554";
            journalName: "Plant J"; date: "2014-07"; volume: "79"; issue: "2"; 
            pages: "348-59"
COMMENT     Sequence Label: pChimera
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        463..538
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(550..574)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(717..734)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agggcgaatt ggcggaaggc cgtcaaggcc acgtgtcttg tccagagctc cctaggcttt
       61 ttttcttctt cttcgttcat acagtttttt tttgtttatc agcttacatt ttcttgaacc
      121 gtagctttcg ttttcttctt tttaactttc cattcggagt ttttgtatct tgtttcatag
      181 tttgtcccag gattagaatg attaggcatc gaaccttcaa gaatttgatt gaataaaaca
      241 tcttcattct taagatatga agataatctt caaaaggccc ctgggaatct gaaagaagag
      301 aagcaggccc atttatatgg gaaagaacaa tagtatttct tatataggcc catttaagtt
      361 gaaaacaatc ttcaaaagtc ccacatcgct tagataagaa aacgaagctg agtttatata
      421 cagctagagt cgaagtagtg attggggtct tcgagaagac ctgttttaga gctagaaata
      481 gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt
      541 tttttctaga cccagctttc ttgtacaaag ttggcattac ctaggggtac ctggagcaca
      601 agactggcct catgggcctt ccgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
      661 cagctgcatt aacatggtca tagctgtttc cttgcgtatt gggcgctctc cgcttcctcg
      721 ctcactgact cgctgcgctc ggtcgttcgg gtaaagcctg gggtgcctaa tgagcaaaag
      781 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtt
//
