LOCUS       sequence-107546-         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The multiple cloning site of pGreenII0000 cloned into binary vector 
            pDHt.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Khang CH, Park SY, Lee YH, Kang S
  TITLE     A dual selection based, targeted gene replacement tool for 
            Magnaporthe grisea and Fusarium oxysporum.
  JOURNAL   Fungal Genet Biol. 2005 Jun;42(6):483-92. Epub 2005 Apr 25.
  PUBMED    15893252
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol."; date: "2005-06"; volume: "42(6)"; pages: "483-92. Epub
            2005 Apr 25"
COMMENT     Sequence Label: pDHt-SK
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    48..222
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     396..413
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    530..551
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(566..571,572..589,590..596)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    604..620
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     609..631
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     628..644
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     628..644
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     663..683
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        665..683
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    696..803
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     720..736
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     720..736
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(770..786)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(771..787)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(811..830)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(812..830)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(840..857)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(840..856)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(849..871)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ttgcggacgt ttttaatgta ctgaattaac gccgaattaa ttcgggggat ctggatttta
       61 gtactggatt ttggttttag gaattagaaa ttttattgat agaagtattt tacaaataca
      121 aatacatact aagggtttct tatatgctca acacatgagc gaaaccctat aggaacccta
      181 attcccttat ctgggaacta ctcacacatt attatggaga aactcgattg gcaagctgct
      241 ctagccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagccttca
      301 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      361 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      421 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
      481 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
      541 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
      601 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
      661 gcgcaattaa ccctcactaa agggaacaaa agctggagct ccaccgcggt ggcggccgct
      721 ctagaactag tggatccccc gggctgcagg aattcgatat caagcttatc gataccgtcg
      781 acctcgaggg ggggcccggt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
      841 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
      901 cttgcagcac atcccccttt
//
