LOCUS       sequence-107547-         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The multiple cloning site of pGreenII0000 cloned into binary vector 
            pDHt.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Khang CH, Park SY, Lee YH, Kang S
  TITLE     A dual selection based, targeted gene replacement tool for 
            Magnaporthe grisea and Fusarium oxysporum.
  JOURNAL   Fungal Genet Biol. 2005 Jun;42(6):483-92. Epub 2005 Apr 25.
  PUBMED    15893252
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol."; date: "2005-06"; volume: "42(6)"; pages: "483-92. Epub
            2005 Apr 25"
COMMENT     Sequence Label: pDHt-SK
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    19..43
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(187..552)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLSI
                     PSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWKL"
     primer_bind     321..343
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     335..352
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     336..352
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     362..381
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        362..380
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    389..496
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     405..421
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     406..422
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(456..472)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(456..472)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(509..529)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(509..527)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(548..564)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(548..564)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(561..583)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    572..588
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(596..602,603..620,621..626))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    641..662
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(779..796)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aacgctcttt tctcttaggt ttacccgcca atatatcctg tcaaacactg atagtttaaa
       61 ctgaaggcgg gaaacgacaa tctgatccaa gctcaagctg ctctagcatt cgccattcag
      121 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagaacat
      181 taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
      241 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
      301 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc
      361 gtaatacgac tcactatagg gcgaattggg taccgggccc cccctcgagg tcgacggtat
      421 cgataagctt gatatcgaat tcctgcagcc cgggggatcc actagttcta gagcggccgc
      481 caccgcggtg gagctccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt
      541 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
      601 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
      661 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
      721 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
      781 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
      841 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgaaggctg
      901 cattaatgaa tcggccaacg c
//
