LOCUS       sequence-107555-         454 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes CRISPR sgRNA for Gateway or conventional cloning of 1-3 
            sgRNAs into pDe-CAS9 or pCAS9-TPC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Schiml S, Fauser F, Puchta H
  TITLE     The CRISPR/Cas system can be used as nuclease for in planta gene 
            targeting and as paired nickases for directed mutagenesis in 
            Arabidopsis resulting in heritable progeny.
  JOURNAL   Plant J. 2014 Oct 18. doi: 10.1111/tpj.12704.
  PUBMED    25327456
REFERENCE   2  (bases 1 to 454)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2014 Oct 18. doi: 10.1111/tpj.12704."
COMMENT     Sequence Label: pEn-C1.1
FEATURES             Location/Qualifiers
     source          1..454
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      32..41
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      36..45
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    135..159
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggcgaattgg gcccgacgtc gcatgctccc ggccgccatg gcggccgcgg gaattcgatt
       61 caaataatga ttttatttga ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat
      121 gcttttttat aatgccaact ttgtacaaaa aagcaggcta cgcgtcctga ggcttttttt
      181 cttcttcttc gttcatacag tttttttttg tttatcagct tacattttct tgaaccgtag
      241 ctttcgtttt cttcttttta actttccatt cggagttttt gtatcttgtt tcatagtttg
      301 tcccaggatt agaatgatta ggcatcgaac cttcaagaat ttgattgaat aaaacatctt
      361 cattcttaag atatgaagat aatcttcaaa aggcccctgg gaatctgaaa gaagagaagc
      421 aggcccattt atatgggaaa gaacaatagt attt
//
