LOCUS       sequence-107561-        1846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for ZAP-70 tyrosine kinase activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Cadra S, Gucciardi A, Valignat MP, Theodoly O, Vacaflores A, Houtman
            JC, Lellouch AC
  TITLE     ROZA-XL, an improved FRET based biosensor with an increased dynamic 
            range for visualizing Zeta Associated Protein 70 kD (ZAP-70) 
            tyrosine kinase activity in live T cells.
  JOURNAL   Biochem Biophys Res Commun. 2015 Feb 28. pii: S0006-291X(15)00357-5.
            doi: 10.1016/j.bbrc.2015.02.117.
  PUBMED    25735979
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem 
            Biophys Res Commun. 2015 Feb 28. pii: S0006-291X(15)00357-5. doi: 
            10.1016/j.bbrc.2015.02.117."
COMMENT     Sequence Label: ROZA-XL
FEATURES             Location/Qualifiers
     source          1..1846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      16..25
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(22..24,25..27,28..540)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     primer_bind     675..696
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tcggatcccg cggccgccac catggtgagc aagggcgagg agctgttcac cggggtggtg
       61 cccatcctgg tcgagctgga cggcgacgta aacggccaca ggttcagcgt gtccggcgag
      121 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
      181 ctgcccgtgc cctggcccac cctcgtgacc accctgacct ggggcgtgca gtgcttcagc
      241 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
      301 gtccaggagc gtaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
      361 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
      421 gacggcaaca tcctggggca caagctggag tacaactaca tcagccacaa cgtctatatc
      481 accgccgaca agcagaagaa cggcatcaag gcccacttca agatccgcca caacatcgag
      541 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
      601 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
      661 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgcccgcat gcataaacca
      721 catccgtggt tttttggcaa aatccccaga gccaaggcag aagaaatgct cagcaaacag
      781 cggcatgacg gggccttcct gatccgagag agcgagagcg ctcctgggga cttctccctg
      841 tccgtcaagt ttggaaatga tgtgcagcac ttcaaggtgc tccgcgacgg agccgggaag
      901 tatttcctgt gggtggtgaa gtttaattct ttgaatgagc tggtagatta ccacagatca
      961 acatccgtgt ccaggaacca gcagatattc ttacgggaca tagaaggcaa taacggggga
     1021 aataacaatg gcgggtctgc aggtaataac gggggaggca gccgggagta tgtgaatgtg
     1081 tcccaggaac tggagctcat ggtgagcaag ggcgaggagc tgttcaccgg cgtggtgccc
     1141 atcctggtgg agctggacgg cgacgtgaac ggccacaagt tcagcgtgag cggcgagggc
     1201 gagggcgacg ccacctacgg caagctgacc ctgaagctgc tgtgcaccac cggcaagctg
     1261 cccgtgccct ggcccaccct ggtgaccacc ctgggctacg gcgtgcagtg cttcgcccgg
     1321 taccccgacc acatgaagca gcacgacttc ttcaagagcg ccatgcccga gggctacgtg
     1381 caggagcgga ccatcttctt caaggacgac ggcaactaca agacccgggc cgaggtgaag
     1441 ttcgagggcg acaccctggt gaaccggatc gagctgaagg gcatcgactt caaggaggac
     1501 ggcaacatcc tgggccacaa gctggagtac aactacaaca gccacaacgt gtacatcacc
     1561 gccgacaagc agaagaacgg catcaaggcc aacttcaaga tccggcacaa catcgaggac
     1621 ggcggcgtgc agctggccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     1681 ctgctgcccg acaaccacta cctgagctac cagagcgccc tgttcaagga ccccaacgag
     1741 aagcgggacc acatggtgct gctggagttc ctgaccgccg ccggcatcac cgagggcatg
     1801 aacgagctgt ataagtaaag atctaagctt tctagaaatc gaattc
//
