LOCUS       sequence-107562-        1908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ""Reporter of Zap-70 Activity"" Fluorescent reporter of ZAP-70 
            tyrosine kinase activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Randriamampita C, Mouchacca P, Malissen B, Marguet D, Trautmann A, 
            Lellouch AC
  TITLE     A novel ZAP-70 dependent FRET based biosensor reveals kinase 
            activity at both the immunological synapse and the antisynapse.
  JOURNAL   PLoS One. 2008 Jan 30;3(1):e1521. doi: 10.1371/journal.pone.0001521.
  PUBMED    18231606
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2008-01-30"; volume: "3(1):e1521. doi"; pages: " 
            10.1371/journal.pone.0001521"
COMMENT     Sequence Label: ROZA
FEATURES             Location/Qualifiers
     source          1..1908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(58..60,61..63,64..576)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     CDS             join(1150..1152,1153..1155,1156..1869)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLKCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
ORIGIN
        1 aagcttgcgg ccgccaccat gggctgtgtc tgcagctcaa accctgaaga tgacaacatg
       61 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
      121 gacgtaaacg gccacaggtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
      181 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
      241 gtgaccaccc tgacctgggg cgtgcagtgc ttcagccgct accccgacca catgaagcag
      301 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgtac catcttcttc
      361 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
      421 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
      481 ctggagtaca actacatcag ccacaacgtc tatatcaccg ccgacaagca gaagaacggc
      541 atcaaggccc acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
      601 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
      661 ctgagcaccc agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg
      721 aaggagttcg tgaccgccgc ccgcatgcat aaaccacatc cgtggttttt tggcaaaatc
      781 cccagagcca aggcagaaga aatgctcagc aaacagcggc atgacggggc cttcctgatc
      841 cgagagagcg agagcgctcc tggggacttc tccctgtccg tcaagtttgg aaatgatgtg
      901 cagcacttca aggtgctccg cgacggagcc gggaagtatt tcctgtgggt ggtgaagttt
      961 aattctttga atgagctggt agattaccac agatcaacat ccgtgtccag gaaccagcag
     1021 atattcttac gggacataga aggcaataac gggggaaata acaatggcgg gtctgcaggt
     1081 aataacgggg gaaataacaa tggcgggtct tgtgaagatt acgtgaatgt tcctgagagt
     1141 gaggagctca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     1201 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     1261 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     1321 tggcccaccc tcgtgaccac cttcggctac ggcctgaagt gcttcgcccg ctaccccgac
     1381 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     1441 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     1501 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     1561 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     1621 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     1681 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     1741 gacaaccact acctgagcta ccagtccaag ctgagcaaag accccaacga gaagcgcgat
     1801 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctcacggcat ggacgagctg
     1861 tacaagtaaa gatctaagct ttcagatcat aatcagccaa tcgaattc
//
