LOCUS       sequence-107565-         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway-Entry vector containing AtU6-26 promoter and sgRNA backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Fauser F, Schiml S, Puchta H
  TITLE     Both CRISPR/Cas-based nucleases and nickases can be used efficiently
            for genome engineering in Arabidopsis thaliana.
  JOURNAL   Plant J. 2014 Jul;79(2):348-59. doi: 10.1111/tpj.12554. Epub 2014 
            Jun 17.
  PUBMED    24836556
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12554";
            journalName: "Plant J"; date: "2014-07"; volume: "79"; issue: "2"; 
            pages: "348-59"
COMMENT     Sequence Label: pEn-Chimera
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      42..51
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      46..55
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    70..169
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_RNA        576..651
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(663..687)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(693..792)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ctcactatag ggcgaattgg gcccgacgtc gcatgctccc ggccgccatg gcggccgcgg
       61 gaattcgatc aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt
      121 gatgagcaat gcttttttat aatgccaact ttgtacaaaa aagcaggctc tttttttctt
      181 cttcttcgtt catacagttt ttttttgttt atcagcttac attttcttga accgtagctt
      241 tcgttttctt ctttttaact ttccattcgg agtttttgta tcttgtttca tagtttgtcc
      301 caggattaga atgattaggc atcgaacctt caagaatttg attgaataaa acatcttcat
      361 tcttaagata tgaagataat cttcaaaagg cccctgggaa tctgaaagaa gagaagcagg
      421 cccatttata tgggaaagaa caatagtatt tcttatatag gcccatttaa gttgaaaaca
      481 atcttcaaaa gtcccacatc gcttagataa gaaaacgaag ctgagtttat atacagctag
      541 agtcgaagta gtgattgggg tcttcgagaa gacctgtttt agagctagaa atagcaagtt
      601 aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg ctttttttct
      661 agacccagct ttcttgtaca aagttggcat taacccagct ttcttgtaca aagttggcat
      721 tataaaaaat aattgctcat caatttgttg caacgaacag gtcactatca gtcaaaataa
      781 aatcattatt tgatc
//
