LOCUS       sequence-107567-         772 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway-Entry vector containing AtU6-26 promoter and sgRNA backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Fauser F, Schiml S, Puchta H
  TITLE     Both CRISPR/Cas-based nucleases and nickases can be used efficiently
            for genome engineering in Arabidopsis thaliana.
  JOURNAL   Plant J. 2014 Jul;79(2):348-59. doi: 10.1111/tpj.12554. Epub 2014 
            Jun 17.
  PUBMED    24836556
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12554";
            journalName: "Plant J"; date: "2014-07"; volume: "79"; issue: "2"; 
            pages: "348-59"
COMMENT     Sequence Label: pEn-Chimera
FEATURES             Location/Qualifiers
     source          1..772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    66..165
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    171..195
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_RNA        complement(207..282)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(689..713)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 catccaacgc gttgggagct ctcccatatg gtcgacctgc aggcggccgc gaattcacta
       61 gtgatcaaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgatg
      121 agcaattatt ttttataatg ccaactttgt acaagaaagc tgggttaatg ccaactttgt
      181 acaagaaagc tgggtctaga aaaaaagcac cgactcggtg ccactttttc aagttgataa
      241 cggactagcc ttattttaac ttgctatttc tagctctaaa acaggtcttc tcgaagaccc
      301 caatcactac ttcgactcta gctgtatata aactcagctt cgttttctta tctaagcgat
      361 gtgggacttt tgaagattgt tttcaactta aatgggccta tataagaaat actattgttc
      421 tttcccatat aaatgggcct gcttctcttc tttcagattc ccaggggcct tttgaagatt
      481 atcttcatat cttaagaatg aagatgtttt attcaatcaa attcttgaag gttcgatgcc
      541 taatcattct aatcctggga caaactatga aacaagatac aaaaactccg aatggaaagt
      601 taaaaagaag aaaacgaaag ctacggttca agaaaatgta agctgataaa caaaaaaaaa
      661 ctgtatgaac gaagaagaag aaaaaaagag cctgcttttt tgtacaaagt tggcattata
      721 aaaaagcatt gctcatcaat ttgttgcaac gaacaggtca ctatcagtca aa
//
