LOCUS       sequence-107575-         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pRS415 backbone, contains an RFP flanked by BsaI sites with CAGT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Agmon N, Mitchell LA, Cai Y, Ikushima S, Chuang J, Zheng A, Choi WJ,
            Martin JA, Caravelli K, Stracquadanio G, Boeke JD
  TITLE     Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae 
            Transcription Units.
  JOURNAL   ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub
            2015 Mar 23.
  PUBMED    25756291
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500372z";
            journalName: "ACS Synth Biol"; date: "2015-07-17- 17"; volume: "4"; 
            issue: "7"; pages: "853-9"
COMMENT     Sequence Label: pAV115.loxP
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    56..89
                     /label=loxPsym
                     /bound_moiety="Cre recombinase"
                     /note="loxPsym has a symmetric spacer region, and loxPsym 
                     sites are capable of recombining in either orientation 
                     (Hoess et al., 1986)."
     CDS             complement(161..832)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="SSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKLK
                     VTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVT
                     VTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKMRL
                     KLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHSTG
                     A"
ORIGIN
        1 actcactata gggcgaattg ggtaccgggc cccccctcga ggtcgacggc agctgataac
       61 ttcgtataat gtacattata cgaagttatg ggacgacctg cagcccccag tagagaccgc
      121 ctggctctag tagcgatcta cactagcact atcagcgtta ttaagcaccg gtggagtgac
      181 gaccttcagc acgttcgtac tgttcaacga tggtgtagtc ttcgttgtgg gaggtgatgt
      241 ccagtttgat gtcggttttg taagcacccg gcagctgaac cggtttttta gccatgtagg
      301 tggttttaac ttcagcgtcg tagtgaccac cgtctttcag tttcagacgc attttgattt
      361 cacctttcag agcaccgtct tccgggtaca tacgttcggt ggaagcttcc caacccatgg
      421 tttttttctg cataaccgga ccgtcggacg ggaagttggt accacgcagt ttaactttgt
      481 agatgaactc accgtcttgc agggaggagt cctgggtaac ggtaacaaca ccaccgtctt
      541 cgaagttcat aacacgttcc catttgaaac cttccgggaa ggacagtttc aggtagtccg
      601 ggatgtcagc cgggtgttta acgtaagctt tggaaccgta ctggaactgc ggggacagga
      661 tgtcccaagc gaacggcagc ggaccacctt tggtaacttt cagtttagcg gtctgggtac
      721 cttcgtacgg acgaccttca ccttcacctt cgatttcgaa ctcgtgaccg ttaacggaac
      781 cttccatacg aactttgaaa cgcatgaact ctttgataac gtcttcggag gaag
//
