LOCUS       sequence-107578-         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a binary Agrobacterium vector carrying the HSVtk gene as a negative 
            selection marker against ectopic transformants..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Khang CH, Park SY, Lee YH, Kang S
  TITLE     A dual selection based, targeted gene replacement tool for 
            Magnaporthe grisea and Fusarium oxysporum.
  JOURNAL   Fungal Genet Biol. 2005 Jun;42(6):483-92. Epub 2005 Apr 25.
  PUBMED    15893252
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol."; date: "2005-06"; volume: "42(6)"; pages: "483-92. Epub
            2005 Apr 25"
COMMENT     Sequence Label: pGKO1
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     81..103
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     95..112
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     96..112
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    116..172
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(181..197)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(181..197)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(194..216)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    205..221
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(229..235,236..253,254..259))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    274..295
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
       61 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct
      121 tgcatgcctg caggtcgact ctagaggatc cccgggtacc gagctcgaat tcgtaatcat
      181 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
      241 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
      301 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
      361 cggccaacgc gcggggagag gcggtttgcg tattggctag agcagcttgc caaagcttat
      421 catcatgcaa catgcatgta ctgtctgatg tattaagagt ataggggtca acaactagag
      481 tgtctgacag gtatatctag cagtttgatt tgaccattct tttattaaaa cactgtaacc
      541 cgcaacgaag aactcctccg cttcaagtca agaatgcgca tatgtgcgta aaatcgacaa
      601 gcccggcggc attccaccga gcagggtatt gcctttggca ctttacgtgc gcagaggagc
      661 ctgaatgttg agtggaatga tgcatgcctg caggtcgact cagttagcct cccccatctc
      721 ccgggcaaac gtgcgcgcca ggtcgcagat cgtcggtatg gagccggggg tggtgacgtg
      781 ggtctggacc atcccggagg taagttgca
//
