LOCUS       sequence-107579-         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Binary expression vector with A.th. codon-optimized Cas9 for 
            conventional cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Fauser F, Schiml S, Puchta H
  TITLE     Both CRISPR/Cas-based nucleases and nickases can be used efficiently
            for genome engineering in Arabidopsis thaliana.
  JOURNAL   Plant J. 2014 Jul;79(2):348-59. doi: 10.1111/tpj.12554. Epub 2014 
            Jun 17.
  PUBMED    24836556
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12554";
            journalName: "Plant J"; date: "2014-07"; volume: "79"; issue: "2"; 
            pages: "348-59"
COMMENT     Sequence Label: pCAS9-TPC
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(63..614)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
ORIGIN
        1 ggaattagaa attttattga tagaagtatt ttacaaatac aaatacatac taagttgggt
       61 catcagatct cggtgacggg caggaccgga cggggcggta ccggcaggct gaagtccagc
      121 tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg cagcatgccg
      181 cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg cagcccgatg
      241 acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg ggtgtagagc
      301 gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga ctcggccgtc
      361 cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc gacctcgccg
      421 tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc gtccgtccac
      481 tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat gtagtggttg
      541 acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc ggccgggcgt
      601 cgttctgggc tcatggtaga tccccaacag atctatcgac taagggtttc ttatatgctc
      661 aacagctgag cgaaacccta taagaaccct aattccctta tctgggaact acttacacaa
      721 tattatagag agagatagat ttgtagagag agactggtga tttcagcgtg tcctctccaa
      781 atgaaatgaa cttccttata tagaggaagg gt
//
