LOCUS       sequence-107581-         755 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  based on pDe-CAS9, encodes Cas9 D10A nickase variant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Fauser F, Schiml S, Puchta H
  TITLE     Both CRISPR/Cas-based nucleases and nickases can be used efficiently
            for genome engineering in Arabidopsis thaliana.
  JOURNAL   Plant J. 2014 Jul;79(2):348-59. doi: 10.1111/tpj.12554. Epub 2014 
            Jun 17.
  PUBMED    24836556
REFERENCE   2  (bases 1 to 755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12554";
            journalName: "Plant J"; date: "2014-07"; volume: "79"; issue: "2"; 
            pages: "348-59"
COMMENT     Sequence Label: pDe-CAS9-D10A
FEATURES             Location/Qualifiers
     source          1..755
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(30..36,37..54,55..60))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(85..209)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(721..741)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tctcgacgga tcctaactca aaatccacac attatacgag ccggaagcat aaagtgtaaa
       61 gcctggggtg cctaatgcgg ccgccatagt gactggatat gttgtgtttt acagtattat
      121 gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt
      181 tctcgttcag cttttttgta caaacttgtt agggataaca ggcctaggga gctcaagcct
      241 atactgtact taacttgatt gcataattac ttgatcatag actcatagta aacttgatta
      301 cacagataag tgaagaaaca aaccaattca agacataacc aaagagaggt gaaagactgt
      361 tttatatgtc taacattgca ccttaatatc acactgttag ttcctttctt acttaaattc
      421 aacccattaa agtaaaaaca acagataata ataatttgag aatgaacaaa aggaccatat
      481 catttattaa ctcttatcca tccatttgca ttttgatgtc cgaaaacaaa aactgaaaga
      541 acacagtaaa ttacaagcag aacaaatgat agaagaaaac agcttttcca atgccataat
      601 actcaaactt agtaggattc tggtgtgtgg gcaatgaaac tgatgcattg aacttgacga
      661 acgttgtcga aaccgatgat acggacgaaa gctgggaggc ctggagctcg gcgcgcctca
      721 aaccttcctc ttcttcttag gatcagccct tgaaa
//
