LOCUS       sequence-107584-        1269 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  G protein Gamma cone(9) subunit tagged with mcherry on the N 
            terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Karunarathne WK, Giri L, Kalyanaraman V, Gautam N
  TITLE     Optically triggering spatiotemporally confined GPCR activity in a 
            cell and programming neurite initiation and extension.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):E1565-74. doi: 
            10.1073/pnas.1220697110. Epub 2013 Mar 11.
  PUBMED    23479634
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1220697110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-04-23- 23"; volume: "110"; issue: "17"; pages: 
            "E1565-74"
COMMENT     Sequence Label: mcherry Gamma 9 ---pcDNA3.1
FEATURES             Location/Qualifiers
     source          1..1269
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(304..321)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(606..625)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     874..892
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 acncnannng tgnngncnnc nagcgtgagc gatagcgggt ttaacgggcc ctctagactc
       61 gagcggccgc cactgtgctg gatatctgca gaattctcag cttattatac aaccgccttt
      121 ttccttgaag ggattcttgt cctcagggat gcctttgaga aaaggatcgt ttcctgcttg
      181 ggcctccacg tactccttga tttcctttcc cgctttggaa atcggaattc ttgtgttttt
      241 cacttctttc ttcagctgct ccacctccat cttcaacagg tccttctcgc tgagatcctg
      301 ggcgtgatgg tgatggtgat gcatggtacc cttgtacagc tcgtccatgc cgccggtgga
      361 gtggcggccc tcggcgcgtt cgtactgttc cacgatggtg tagtcctcgt tgtgggaggt
      421 gatgtccaac ttgatgttga cgttgtaggc gccgggcagc tgcacgggct tcttggcctt
      481 gtaggtggtc ttgacctcag cgtcgtagtg gccgccgtcc ttcagcttca gcctctgctt
      541 gatctcgccc ttcagggcgc cgtcctcggg gtacatccgc tcggaggagg cctcccagcc
      601 catggtcttc ttctgcatta cggggccgtc ggaggggaag ttggtgccgc gcagcttcac
      661 cttgtagatg aactcgccgt cctgcaggga ggagtcctgg gtcacggtca ccacgccgcc
      721 gtcctcgaag ttcatcacgc gctcccactt gaagccctcg gggaaggaca gcttcaagta
      781 gtcggggatg tcggcggggt gcttcacgta ggccttggag ccgtacatga actgagggga
      841 caggatgtcc caggcgaagg gcaggggcca cccttggtca ccttcagctt ggcggtctgg
      901 gtgccctcgt aggggcggcc ctcgccctcg ccctcgatct cgactcgtgg ccgttcacgg
      961 agccctccat gtgcacttga gcgcatgact cctgatgatg gccatgtatc ctcctcgcct
     1021 nctcacatan ctagtttaac gctagcagct gggtctccta tagtgagtcg attatttcga
     1081 tagcagtagc angggnntct agtagcngag agctntgcta tnngactcnc gtacnctacg
     1141 cattncgtca tgggcgnatn tacgnnntng anncntgatt tgngncaacn nnnnngccna
     1201 ngggnnantt gnnannnnnn naacnnntcn nnnnntncac nnnnnnannn natannnnnn
     1261 cnccngnna
//
