LOCUS       sequence-107585-         721 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Binary expression vector with A.th. codon-optimized Cas9 for 
            conventional cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Fauser F, Schiml S, Puchta H
  TITLE     Both CRISPR/Cas-based nucleases and nickases can be used efficiently
            for genome engineering in Arabidopsis thaliana.
  JOURNAL   Plant J. 2014 Jul;79(2):348-59. doi: 10.1111/tpj.12554. Epub 2014 
            Jun 17.
  PUBMED    24836556
REFERENCE   2  (bases 1 to 721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12554";
            journalName: "Plant J"; date: "2014-07"; volume: "79"; issue: "2"; 
            pages: "348-59"
COMMENT     Sequence Label: pCAS9-TPC
FEATURES             Location/Qualifiers
     source          1..721
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gatatgaata taggcatatc cgtatccgaa ttatccgttt gacagctagc aacgattgta
       61 caattgcttc tttaaaaaag gaagaaagaa agaaagaaaa gaatcaacat cagcgttaac
      121 aaacggcccc gttacggccc aaacggtcat atagagtaac ggcgttaagc gttgaaagac
      181 tcctatcgaa atacgtaacc gcaaacgtgt catagtcaga tcccctcttc cttcaccgcc
      241 tcaaacacaa aaataatctt ctacagccta tatatacaac ccccccttct atctctcctt
      301 tctcacaatt catcatcttt ctttctctac ccccaatttt aagaaatcct ctcttctcct
      361 cttcattttc aaggtaaatc tctctctctc tctctctctc tgttattcct tgttttaatt
      421 aggtatgtat tattgctagt ttgttaatct gcttatctta tgtatgcctt atgtgaatat
      481 ctttatcttg ttcatctcat ccgtttagaa gctataaatt tgttgatttg actgtgtatc
      541 tacacgtggt tatgtttata tctaatcaga tatgaatttc ttcatattgt tgcgtttgtg
      601 tgtaccaatc cgaaatcgtt gatttttttc atttaatcgt gtagctaatt gtacgtatac
      661 atatggatct acgtatcaat tgttcatctg tttgtgtttg tatgtataca gatctgaaaa
      721 a
//
